FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001320024

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001320024
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2581645
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA110470.4279054633770329No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA97440.37743376800450873No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91730.35531608722345637No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA75360.291906904318758No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67760.2624683099341699No Hit
GTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACAGAAGC64690.2505766672025007No Hit
CCTTTATTGCAGCCCACCGGCAGCACTTCGAGGCAATCCGTGGCGGAAAA62710.2429071386654633No Hit
GGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTT62160.24077671407184176No Hit
ATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGTTCCGTTGCCGGTAA61880.23969213427872538No Hit
GTGTAAGATCGTCGTGATCGCCACAGAAGCAGATCTTGGTGACGCTGCCG59540.23062814600768117No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCA57070.22106060283269002No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTG55390.21455312407399157No Hit
CGCCTAATGCTTCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGA52750.20432708602460833No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC52300.20258401135709983No Hit
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCG47890.18550187961551645No Hit
GCTATAGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACC44460.17221577714984052No Hit
GCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATTGCCTCGA41660.16136997921867646No Hit
TGCTGATACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATCACGATG41140.15935575960288886No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39190.15180243604368532No Hit
ACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCAT38030.1473091769007745No Hit
CGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTTCGAGGCAAT37510.1452949572849869No Hit
GCCTTTGGTGATGCGATGAACGATCGCGAAATGTTAGTCAGCGTCGGTAG36180.14014320326768398No Hit
CTTCGAGGCAATCCGTGGCGGAAAAACACAAATGTGCACGCTCGCCTAAT34960.13541753416910535No Hit
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTT33860.13115668498186234No Hit
CCGGTAAATGCGGGAGCTCCGCGCGCAGTTGCGGCATCGCATTGCCCATA33270.12887132041779564No Hit
CCACAGAAGCAGATCTTGGTGACGCTGCCGAGTGGCATTTTTTTGACATC32720.1267408958241741No Hit
TCCCATTGCTGATACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATC32140.12449426625271871No Hit
GGTAAATCATCACGATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGT31840.1233322164743797No Hit
CCTAAATGTTGGGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCAC31380.12155040681425991No Hit
CCGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTTCGAGGCAA31190.1208144419546452No Hit
ATACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATCACGATGTAAAA30430.11787058251618639No Hit
ATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACAGAAGCAGATCTTGGT30170.11686347270829257No Hit
TCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACAGAA30000.11620497783390049No Hit
GCATTGACGGTGCTGACCCAACATTTAGGTTTATCGTTGCGCGATTGCAC29340.11364846832155467No Hit
CTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCA29230.11322238340283036No Hit
ACCTAAATGTTGGGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCA28900.11194412864665745No Hit
GGATGGCACTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCT28830.11167298369837836No Hit
AGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACCAACCG27880.10799315940030485No Hit
CTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTA27840.10783821942985965No Hit
GCATATTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTACCGGCAACG27710.10733466452591274No Hit
GTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCG27650.10710225457024494No Hit
CAGCTATACGAAGCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCAC27140.10512676994706863No Hit
GTGCTGACCCAACATTTAGGTTTATCGTTGCGCGATTGCACGGCCTTTGG27040.10473942002095563No Hit
ACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACGGG26850.10400345516134092No Hit
CAGTTGCGGCATCGCATTGCCCATAATAAATCCGCTACCGACGCTGACTA26290.10183429557510812No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)