FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001320194

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001320194
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1988922
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA173930.874493821276048No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA148700.7476411845210622No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA140700.7074183904647844No Hit
ATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGTTCCGTTGCCGGTAA137160.6896198040948815No Hit
GTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACAGAAGC114310.5747334485716382No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTG108900.5475327840910805No Hit
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCG105310.5294828052583258No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCA101810.5118853328587043No Hit
GTGTAAGATCGTCGTGATCGCCACAGAAGCAGATCTTGGTGACGCTGCCG101010.5078630534530766No Hit
CCTTTATTGCAGCCCACCGGCAGCACTTCGAGGCAATCCGTGGCGGAAAA98890.49720401302816297No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC95600.4806623889725188No Hit
GGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTT90920.4571320544495963No Hit
GCTATAGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACC87260.43873012616884927No Hit
CGCCTAATGCTTCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGA83590.42027791939553183No Hit
ACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCAT78340.39388171079609957No Hit
TGCTGATACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATCACGATG72040.3622062604767809No Hit
GGTAAATCATCACGATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGT70670.35531810699464333No Hit
CCGGTAAATGCGGGAGCTCCGCGCGCAGTTGCGGCATCGCATTGCCCATA68920.3465193707948326No Hit
GCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATTGCCTCGA67290.338323976505866No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67280.33827369801329565No Hit
CTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCA65630.3299777467391884No Hit
CTTCGAGGCAATCCGTGGCGGAAAAACACAAATGTGCACGCTCGCCTAAT60830.3058440703054217No Hit
TCCCATTGCTGATACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATC59590.2996095372266987No Hit
ATACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATCACGATGTAAAA59520.29925758777870626No Hit
CCTAAATGTTGGGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCAC58380.2935258396256867No Hit
AGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACCAACCG58260.29292249771484247No Hit
ACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACGGG57320.2881963194132299No Hit
AGAATATGCTGCATCTCCAGCGCATGACGCCCCGTGGCAAAAGTGAGGGT56830.2857326732772829No Hit
GGATGGCACTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCT56530.28422431850017243No Hit
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTT56360.28336958412647656No Hit
CTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTA55900.2810567734682406No Hit
ACCTAAATGTTGGGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCA55900.2810567734682406No Hit
CCACAGAAGCAGATCTTGGTGACGCTGCCGAGTGGCATTTTTTTGACATC54390.27346472109011816No Hit
GCATATTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTACCGGCAACG53860.27079996098388975No Hit
TCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACAGAA53590.2694424416844904No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52970.2663251751451289No Hit
TCGATTATCTGATAACGAAAACCGCTATAGACAAATGCCTGCAACAACGC52930.2661240611748475No Hit
GCCTTTGGTGATGCGATGAACGATCGCGAAATGTTAGTCAGCGTCGGTAG52780.2653698837862923No Hit
CGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTTCGAGGCAAT52290.26290623765034526No Hit
CCTAATGCTTCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATC52220.2625542882023528No Hit
ATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACAGAAGCAGATCTTGGT49610.24943160164149222No Hit
GTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCG49500.24887853822321843No Hit
GCATTGACGGTGCTGACCCAACATTTAGGTTTATCGTTGCGCGATTGCAC47670.23967757408284487No Hit
CCGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTTCGAGGCAA47180.23721392794689788No Hit
GCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACCAACCGTCGTCATT47050.23656030754348337No Hit
GATCTTGGTGACGCTGCCGAGTGGCATTTTTTTGACATCGATTATCTGAT44850.22549903917800698No Hit
GAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCAT43920.2208231393689647No Hit
GCACTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACT43650.21946562006956533No Hit
TGCGGGAGCTCCGCGCGCAGTTGCGGCATCGCATTGCCCATAATAAATCC43060.21649918900791482No Hit
GTGCTGACCCAACATTTAGGTTTATCGTTGCGCGATTGCACGGCCTTTGG42800.21519194820108584No Hit
CAGTTGCGGCATCGCATTGCCCATAATAAATCCGCTACCGACGCTGACTA41710.20971159251091798No Hit
CAGCTATACGAAGCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCAC41240.20734850336011165No Hit
GGTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTC40900.20563903461271985No Hit
ACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCT40320.2027228820436397No Hit
GCCTAATGCTTCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGAT40170.2019687046550845No Hit
ATTATGGGCAATGCGATGCCGCAACTGCGCGCGGAGCTCCCGCATTTACC40100.20161675520709207No Hit
GATATGGATGGCACTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAAC40040.20131508425167No Hit
ACACAAATGTGCACGCTCGCCTAATGCTTCGTATAGCTGGATCTGCAAGC39890.20056090686311479No Hit
CTATCGCTGGATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTC39240.19729280484604225No Hit
TCCAGCGATAGCGCCCCGAGAATATGCTGCATCTCCAGCGCATGACGCCC38890.19553305760608009No Hit
AATGTGCACGCTCGCCTAATGCTTCGTATAGCTGGATCTGCAAGCGTGTA37990.19100799327474882No Hit
CAACAACGCAGGGATCTCTTTCCCGGTAAACCAACCGTCGTCATTGAAGA37580.18894657507936458No Hit
CGGGAGCTCCGCGCGCAGTTGCGGCATCGCATTGCCCATAATAAATCCGC37140.1867343214062693No Hit
CCGCTATAGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAA37080.18643265045084725No Hit
GCTTCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACA36540.1837176118520485No Hit
ATCAAATACGCATCCAGCGATAGCGCCCCGAGAATATGCTGCATCTCCAG36500.1835164978817671No Hit
ATGCACGCGCGTTCCGTTGCCGGTAATCAAATACGCATCCAGCGATAGCG36350.1827623204932119No Hit
GAAGATATGCATGCTGGCTCGGGTATCCCATTGCTGATACAGCACCAGCT36350.1827623204932119No Hit
ACCAAGATCTGCTTCTGTGGCGATCACGACGATCTTACACGCTTGCAGAT35570.17884059807272482No Hit
GCTGATACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATCACGATGT35360.17778474972874753No Hit
GGGTATCCCATTGCTGATACAGCACCAGCTCCGCGACATCCGCAGGTAAA35270.17733224329561442No Hit
CTATAGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACCA34750.17471776168195635No Hit
CTTCTGTGGCGATCACGACGATCTTACACGCTTGCAGATCCAGCTATACG34600.17396358429340114No Hit
CGTGTAAGATCGTCGTGATCGCCACAGAAGCAGATCTTGGTGACGCTGCC34410.17300829293456454No Hit
ATGCATGCTGGCTCGGGTATCCCATTGCTGATACAGCACCAGCTCCGCGA34270.1723043940385797No Hit
CTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCGGGGCG34160.17175133062030587No Hit
GGGTAATGTCGCGTTCACGCAGTCGCGCCAAAGTAGAGAGGGTTTTCTCA34110.17149993815745412No Hit
GAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCT33810.16999158338034373No Hit
GTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACG33750.16968991242492162No Hit
CTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCACATTTGTGTT33690.16938824146949957No Hit
GGCTCGGGTATCCCATTGCTGATACAGCACCAGCTCCGCGACATCCGCAG33550.1686843425735147No Hit
ATACGAAGCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATT32950.16566763301929388No Hit
CATTTGTGTTTTTCCGCCACGGATTGCCTCGAAGTGCTGCCGGTGGGCTG32480.16330454386848756No Hit
CCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTT32340.16260064497250268No Hit
GCATTTGATATGGATGGCACTTTATTGGTGCCCGACCATCATTTAGGTGA32220.16199730306165852No Hit
GTGCACGCTCGCCTAATGCTTCGTATAGCTGGATCTGCAAGCGTGTAAGA32220.16199730306165852No Hit
CTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCG31900.1603883912994074No Hit
GATCTCTTTCCCGGTAAACCAACCGTCGTCATTGAAGATATGCATGCTGG31630.15903087200000804No Hit
GTTGCGGCATCGCATTGCCCATAATAAATCCGCTACCGACGCTGACTAAC31570.15872920104458596No Hit
GCACCAGCTCCGCGACATCCGCAGGTAAATCATCACGATGTAAAAGTTCA31510.15842753008916388No Hit
AGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACAGAAGCAGAT31370.157723631193179No Hit
TCATTGAAGATATGCATGCTGGCTCGGGTATCCCATTGCTGATACAGCAC30620.15395274425040298No Hit
GAATGCACGCGCGTTCCGTTGCCGGTAATCAAATACGCATCCAGCGATAG30440.15304773138413674No Hit
GATCTGCTTCTGTGGCGATCACGACGATCTTACACGCTTGCAGATCCAGC30310.15239411098072222No Hit
CAATAAAGGCGCTGCATTGACGGTGCTGACCCAACATTTAGGTTTATCGT30160.15163993359216701No Hit
GAAGCAGATCTTGGTGACGCTGCCGAGTGGCATTTTTTTGACATCGATTA30100.15133826263674494No Hit
CCTTCCAGAGAATGCACGCGCGTTCCGTTGCCGGTAATCAAATACGCATC29930.15048352826304903No Hit
GCATTGCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATC29260.14711486926083575No Hit
CATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATTGCCTCGAA29250.14706459076826545No Hit
ATCGCTGGATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTC29250.14706459076826545No Hit
CGATTATCTGATAACGAAAACCGCTATAGACAAATGCCTGCAACAACGCA29230.14696403378312473No Hit
GTCATGCGCTGGAGATGCAGCATATTCTCGGGGCGCTATCGCTGGATGCG29220.14691375529055437No Hit
CAGCGATAGCGCCCCGAGAATATGCTGCATCTCCAGCGCATGACGCCCCG28180.14168479206323828No Hit
CTTCCAGAGAATGCACGCGCGTTCCGTTGCCGGTAATCAAATACGCATCC27900.14027699427126855No Hit
AATCATCACGATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGTTCCG27310.1373105632096181No Hit
GCTCCGCGACATCCGCAGGTAAATCATCACGATGTAAAAGTTCACCTTCC27250.137008892254196No Hit
ATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGC27220.13685805677648497No Hit
TATGCATGCTGGCTCGGGTATCCCATTGCTGATACAGCACCAGCTCCGCG27180.13665694280620358No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27070.13610387938792973No Hit
GGTAAATGCGGGAGCTCCGCGCGCAGTTGCGGCATCGCATTGCCCATAAT27020.135852486925078No Hit
CGATAAACCTAAATGTTGGGTCAGCACCGTCAATGCAGCGCCTTTATTGC27000.13575192993993732No Hit
ACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGC26960.13555081596965593No Hit
CCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCAC26930.13539998049194488No Hit
GGTATCCCATTGCTGATACAGCACCAGCTCCGCGACATCCGCAGGTAAAT26730.13439441064053795No Hit
GCCTCGAAGTGCTGCCGGTGGGCTGCAATAAAGGCGCTGCATTGACGGTG26660.1340424611925455No Hit
TTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTACCGGCAACGGAACG26600.13374079023712343No Hit
CTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACG26430.13288605586342753No Hit
TATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGT26310.13228271395258334No Hit
GTCTGGCAGCATTTGATATGGATGGCACTTTATTGGTGCCCGACCATCAT26060.13102575163832467No Hit
GGAGATGCAGCATATTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTA25840.12991962480177704No Hit
GGGATCTCTTTCCCGGTAAACCAACCGTCGTCATTGAAGATATGCATGCT25710.12926600439836253No Hit
GTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAAC25660.12901461193551078No Hit
ATGCGGGAGCTCCGCGCGCAGTTGCGGCATCGCATTGCCCATAATAAATC25550.12846154851723698No Hit
ACGATAAACCTAAATGTTGGGTCAGCACCGTCAATGCAGCGCCTTTATTG25400.12770737112868177No Hit
GATATGCATGCTGGCTCGGGTATCCCATTGCTGATACAGCACCAGCTCCG25130.12634985182928238No Hit
GTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACT25100.12619901635157135No Hit
GCTATACGAAGCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGG25040.12589734539614927No Hit
ATAGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACCAAC24810.12474094006703128No Hit
ATGTTAGTCAGCGTCGGTAGCGGATTTATTATGGGCAATGCGATGCCGCA24740.12438899061903885No Hit
CTGCAAGCGTGTAAGATCGTCGTGATCGCCACAGAAGCAGATCTTGGTGA24630.12383592720076504No Hit
CCCCGAGAATATGCTGCATCTCCAGCGCATGACGCCCCGTGGCAAAAGTG24620.1237856487081947No Hit
GCAATAAAGGCGCTGCATTGACGGTGCTGACCCAACATTTAGGTTTATCG24500.12318230679735052No Hit
CACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCGGGG24080.12107061010939593No Hit
AAGCAGATCTTGGTGACGCTGCCGAGTGGCATTTTTTTGACATCGATTAT23660.11895891342144137No Hit
CCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTG23060.11594220386722054No Hit
GCGCATGACGCCCCGTGGCAAAAGTGAGGGTAATGTCGCGTTCACGCAGT23050.11589192537465019No Hit
TTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGC22990.1155902544192281No Hit
CATTTGATATGGATGGCACTTTATTGGTGCCCGACCATCATTTAGGTGAG22840.11483607703067289No Hit
GTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACT22820.11473552004553221No Hit
GTTCATCGCATCACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAAT22750.11438357059753977No Hit
CTTTCCCGGTAAACCAACCGTCGTCATTGAAGATATGCATGCTGGCTCGG22540.11332772225356247No Hit
CTCCAGCGCATGACGCCCCGTGGCAAAAGTGAGGGTAATGTCGCGTTCAC22400.11262382335757762No Hit
ACATTTAGGTTTATCGTTGCGCGATTGCACGGCCTTTGGTGATGCGATGA22380.11252326637243693No Hit
CACCAGCTCCGCGACATCCGCAGGTAAATCATCACGATGTAAAAGTTCAC22370.11247298787986659No Hit
CTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCACA22120.1112160255656079No Hit
ACGCAGGGATCTCTTTCCCGGTAAACCAACCGTCGTCATTGAAGATATGC21960.11041156968448235No Hit
TCACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAG21930.11026073420677132No Hit
GCCGAGTGGCATTTTTTTGACATCGATTATCTGATAACGAAAACCGCTAT21910.11016017722163061No Hit
GCATCGCATTGCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCG21840.10980822777363819No Hit
ATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGAC21750.10935572134050506No Hit
GCGTGAACGCGACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGG21680.10900377189251263No Hit
TGGTGACGCTGCCGAGTGGCATTTTTTTGACATCGATTATCTGATAACGA21580.10850098696680915No Hit
GATTTATTATGGGCAATGCGATGCCGCAACTGCGCGCGGAGCTCCCGCAT21540.10829987299652777No Hit
CTCTTTCCCGGTAAACCAACCGTCGTCATTGAAGATATGCATGCTGGCTC21440.1077970880708243No Hit
GCGTGTAAGATCGTCGTGATCGCCACAGAAGCAGATCTTGGTGACGCTGC21080.1059870623382918No Hit
CATCCAGCGATAGCGCCCCGAGAATATGCTGCATCTCCAGCGCATGACGC20970.10543399892001798No Hit
CGCTGGATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTG20530.1032217452469227No Hit
CGACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGC20380.1024675678583675No Hit
TACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATCACGATGTAAAAG20370.10241728936579715No Hit
TCGCATCACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGG20070.10090893458868674No Hit
TGTTGGGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAG19890.10000392172242048No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)