FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001320215

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001320215
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3536997
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT98320.2779759213818954No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC65440.18501570682700608No Hit
GCCCCACTCTGTGTCGGAGTCTCCGGGGAAGACGGTAACCATCTCCTGCA59980.1695788828771978No Hit
TCTCTGGACTGAAGACTGAGGACGAGGCTGACTACTACTGTCAGTCTTAT57780.16335891718313586No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGAAGCCTGA56600.16002275376541172No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53170.15032526179694244No Hit
GTCCAGAGATGGTGAGGGAGGCAGAGTTGGAGGAGCTGTCGATGGAGCCA51080.1444162943875836No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA50430.14257857725070167No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA49990.14133458411188926No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT47260.13361617213698512No Hit
GTGTCGGAGTCTCCGGGGAAGACGGTAACCATCTCCTGCACCCGCAGCAG47220.13350308185163856No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA46960.13276799499688577No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA46150.1304779167186175No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA45890.12974282986386473No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC45390.12832920129703249No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG44980.12717002587223003No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC44850.12680248244485362No Hit
GTCTTATGATAGCAGCAATCAGGCTTCTTGGGTGTTCGGCGGAGGGACCA44370.12544539902069468No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT44300.12524749102133817No Hit
ACCAAAGACCCTCTGGGGTCCCTGATCGGTTCTCTGGCTCCATCGACAGC43910.124144860739209No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43490.1229574127430699No Hit
GTAGTAGTCAGCCTCGTCCTCAGTCTTCAGTCCAGAGATGGTGAGGGAGG40030.11317510306059066No Hit
TTCATACTCTAAGTATCAAAATCTTCCAATTATCATGCTCACCTGAAAGA39850.11266619677653106No Hit
TAACAGACTTGGAAGCAGATGATACAGACTTCTTTTTTTCATAATCAGGT39830.11260965163385776No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCA36730.10384515451949776No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT36360.1027990693800419No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG36130.10214880023929904No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCA36050.10192261966860587No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)