FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001320216

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001320216
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3536997
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT101190.2860901493555126No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCC61920.17506376171650698No Hit
GCCCCACTCTGTGTCGGAGTCTCCGGGGAAGACGGTAACCATCTCCTGCA57070.16135156461823405No Hit
TCTCTGGACTGAAGACTGAGGACGAGGCTGACTACTACTGTCAGTCTTAT55520.15696931606105405No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAGAAGCCTGA53220.15046662465362567No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA50460.1426633949647116No Hit
GTCCAGAGATGGTGAGGGAGGCAGAGTTGGAGGAGCTGTCGATGGAGCCA49380.1396099572603539No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA47380.13395544299302486No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46780.13225908871282616No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT46430.1312695487160436No Hit
GTCTTATGATAGCAGCAATCAGGCTTCTTGGGTGTTCGGCGGAGGGACCA45640.1290360155804486No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA45070.12742447901425985No Hit
GTGTCGGAGTCTCCGGGGAAGACGGTAACCATCTCCTGCACCCGCAGCAG43510.1230139578857432No Hit
CCTATGAACATTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCA43360.1225898693156935No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCGCCCTCCTCTGAGGAGC43020.12162860189024757No Hit
CCTCAGAGGAGGGCGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA42930.12137414874821777No Hit
ACCAAAGACCCTCTGGGGTCCCTGATCGGTTCTCTGGCTCCATCGACAGC41960.11863170932856319No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG41480.11727462590440423No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT41330.11685053733435453No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC40000.11309028534658074No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA39430.11147874878039196No Hit
GTAGTAGTCAGCCTCGTCCTCAGTCTTCAGTCCAGAGATGGTGAGGGAGG39010.11029130078425285No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA38000.1074357710792517No Hit
TAACAGACTTGGAAGCAGATGATACAGACTTCTTTTTTTCATAATCAGGT37720.10664413908182563No Hit
TTCATACTCTAAGTATCAAAATCTTCCAATTATCATGCTCACCTGAAAGA36980.10455196880291388No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36900.10432578823222072No Hit
GTTCCCGCCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT36110.10209225509662576No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCA35860.10138544081320963No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)