FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001320241

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001320241
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3312026
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT107050.3232160617096605No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT105440.3183549887591462No Hit
ATATTATTGTCTGCAGTGTGATGATGTCCCCCTCACTTTCGGCGGAGGGA96560.2915436050320861No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC96270.2906680080409997No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA86560.2613506053394508No Hit
CAATAATATGTTGCAATGTCTTCCGGCTGCAGGCTGTTGATGGTTAAAGT72560.21908040576976145No Hit
GTGTGATGATGTCCCCCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCA69060.20851285587733911No Hit
TCATCACACTGCAGACAATAATATGTTGCAATGTCTTCCGGCTGCAGGCT67250.20304792293297214No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT63270.1910311090553033No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG54940.16588034031133814No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC52820.15947942437649945No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG48660.1469191365043632No Hit
ATAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATCAACAC48410.1461643115120473No Hit
TTCATACTCTAAGTATCAAAATCTTCCAATTATCATGCTCACCTGAAAGA48120.14528871452096087No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG47990.14489620552495663No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG47540.14353752053878804No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47470.1433261695409396No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT46750.14115227356306986No Hit
CACTTTACTTTAACCATCAACAGCCTGCAGCCGGAAGACATTGCAACATA46680.1409409225652214No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC46650.1408503435661435No Hit
ACCAGGGACAGCCCCTAAGCTCCTGATCTACGATGCATCCACTTTGGAGA45730.13807258759442106No Hit
GTGATGATGTCCCCCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAA44400.13405691863530056No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC43000.12982989867833164No Hit
AACCAGGGACAGCCCCTAAGCTCCTGATCTACGATGCATCCACTTTGGAG42800.12922603868447893No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41730.12599538771736696No Hit
GTCCCTGGTTTGTGCTGAAACCAATTCAAATAGGTGTTGATGTCCTGACT41680.12584442271890378No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG40500.12228164875517282No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC38530.11633362781572366No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT37670.11373702984215704No Hit
CTCTTAGGAATACAGTTTCTAGCATTAAACAAATAAACAAGGGGAGAAAA37490.1131935558476896No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT37490.1131935558476896No Hit
TAACAGACTTGGAAGCAGATGATACAGACTTCTTTTTTTCATAATCAGGT36990.11168390586305783No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA36230.10938923788641756No Hit
TTATTACACCTCCTGATTTTCAGTCCTTGAGTTTTATTTTCTCCCCTTGT35390.10685302591223621No Hit
TTATTACACCTCCTGATTTTCACTCCTTGAGTTTTATTTTCTCCCCTTGT34750.10492067393190754No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC34600.10446777893651801No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG34050.10280716395342307No Hit
GTGTAAGAAATTGCCATTTGAAACAATCCATTTTGTAACTGAACCTTATG33350.1006936539749386No Hit
TATTTGGACATAACAGACTTGGAAGCAGATGATACAGACTTCTTTTTTTC33200.10024075897954908No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)