FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001320242

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001320242
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3312026
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT99810.3013563299321926No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT99270.2997259079487903No Hit
ATATTATTGTCTGCAGTGTGATGATGTCCCCCTCACTTTCGGCGGAGGGA97490.29435155400350116No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC89160.269200785259536No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA84360.2547081454070711No Hit
CAATAATATGTTGCAATGTCTTCCGGCTGCAGGCTGTTGATGGTTAAAGT70480.21280026183369333No Hit
GTGTGATGATGTCCCCCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCA66190.19984746496555283No Hit
TCATCACACTGCAGACAATAATATGTTGCAATGTCTTCCGGCTGCAGGCT62470.1886156690798925No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT58700.177232908195769No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG51180.15452777242690727No Hit
CACTTTACTTTAACCATCAACAGCCTGCAGCCGGAAGACATTGCAACATA46920.14166555455784466No Hit
TTCATACTCTAAGTATCAAAATCTTCCAATTATCATGCTCACCTGAAAGA46410.14012571157352027No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC45870.13849528959011795No Hit
ATAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATCAACAC45810.13831413159196215No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC45480.13731776260210518No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG44560.13454000663038274No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA43750.13209437365527926No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT43500.13133954866296338No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA43400.13103761866603703No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43370.13094703966695914No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG43310.13076588166880335No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG43050.1299808636767948No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC42810.12925623168417155No Hit
GTGATGATGTCCCCCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAA41140.12421400073550147No Hit
AACCAGGGACAGCCCCTAAGCTCCTGATCTACGATGCATCCACTTTGGAG41050.12394226373826775No Hit
GTCCCTGGTTTGTGCTGAAACCAATTCAAATAGGTGTTGATGTCCTGACT40750.1230364737474887No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG40280.12161740276193485No Hit
ACCAGGGACAGCCCCTAAGCTCCTGATCTACGATGCATCCACTTTGGAGA39450.11911138378744611No Hit
CTCTTAGGAATACAGTTTCTAGCATTAAACAAATAAACAAGGGGAGAAAA37460.11310297684861169No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA37290.11258969585383691No Hit
TAACAGACTTGGAAGCAGATGATACAGACTTCTTTTTTTCATAATCAGGT36580.1104459928756598No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT35810.10812113189932687No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT35140.10609820091992031No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA35050.1058264639226866No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC34990.10564530592453078No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC34820.105132024929756No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34620.10452816493590328No Hit
GGACATAACAGACTTGGAAGCAGATGATACAGACTTCTTTTTTTCATAAT33990.10262600595526726No Hit
GTGTAAGAAATTGCCATTTGAAACAATCCATTTTGTAACTGAACCTTATG33570.10135789996817658No Hit
CTTCTTAATTATATGTTCATACTCTAAGTATCAAAATCTTCCAATTATCA33210.10027095197924171No Hit
TTATTACACCTCCTGATTTTCAGTCCTTGAGTTTTATTTTCTCCCCTTGT33210.10027095197924171No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG33180.1001803729801638No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)