FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001320243

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001320243
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3362089
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT108660.32319192026148025No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT106430.3165591392732316No Hit
ATATTATTGTCTGCAGTGTGATGATGTCCCCCTCACTTTCGGCGGAGGGA99860.2970177172585259No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC95750.28479317471964605No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA87470.2601656291668662No Hit
CAATAATATGTTGCAATGTCTTCCGGCTGCAGGCTGTTGATGGTTAAAGT73400.21831664777464246No Hit
GTGTGATGATGTCCCCCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCA71360.2122489916239576No Hit
TCATCACACTGCAGACAATAATATGTTGCAATGTCTTCCGGCTGCAGGCT67150.1997270149600442No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT64810.19276705643425857No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG56590.1683179713564989No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC54140.16103083529317636No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50750.15094781845453822No Hit
ATAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATCAACAC50560.15038269361697443No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG49700.14782476014168572No Hit
CACTTTACTTTAACCATCAACAGCCTGCAGCCGGAAGACATTGCAACATA49680.147765273316679No Hit
TTCATACTCTAAGTATCAAAATCTTCCAATTATCATGCTCACCTGAAAGA49150.14618887245400106No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG47940.14258991954109482No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG47180.1403294201908397No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC47170.14029967677833632No Hit
GTGATGATGTCCCCCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAA45970.13673046727793345No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT45380.13497560594023536No Hit
ACCAGGGACAGCCCCTAAGCTCCTGATCTACGATGCATCCACTTTGGAGA44470.13226895540242986No Hit
AACCAGGGACAGCCCCTAAGCTCCTGATCTACGATGCATCCACTTTGGAG44130.13125767937731572No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42870.1275100094018927No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG41840.12444643791404689No Hit
GTCCCTGGTTTGTGCTGAAACCAATTCAAATAGGTGTTGATGTCCTGACT41560.12361362236395289No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC41550.12358387895144954No Hit
CTCTTAGGAATACAGTTTCTAGCATTAAACAAATAAACAAGGGGAGAAAA39210.11662392042566393No Hit
TAACAGACTTGGAAGCAGATGATACAGACTTCTTTTTTTCATAATCAGGT39130.11638597312563707No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC38810.11543418392552962No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT38450.11436342107540877No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT38150.11347111870030806No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA37610.11186497442512676No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG35510.10561885779942173No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC35240.10481578566183108No Hit
TATTTGGACATAACAGACTTGGAAGCAGATGATACAGACTTCTTTTTTTC35180.10463732518681094No Hit
GTGTAAGAAATTGCCATTTGAAACAATCCATTTTGTAACTGAACCTTATG35120.1044588647117908No Hit
TTATTACACCTCCTGATTTTCAGTCCTTGAGTTTTATTTTCTCCCCTTGT34660.10309066773663636No Hit
TTATTACACCTCCTGATTTTCACTCCTTGAGTTTTATTTTCTCCCCTTGT34660.10309066773663636No Hit
GGACATAACAGACTTGGAAGCAGATGATACAGACTTCTTTTTTTCATAAT34580.10285272043660951No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC33710.1002650435488174No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA33690.1002055567238107No Hit
ATAATATGTTGCAATGTCTTCCGGCTGCAGGCTGTTGATGGTTAAAGTAA33650.10008658307379727No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)