FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001320244

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001320244
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3362089
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT105910.315012481823057No Hit
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT102060.3035612680092645No Hit
ATATTATTGTCTGCAGTGTGATGATGTCCCCCTCACTTTCGGCGGAGGGA98420.2927346658580424No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC89500.2662035419050477No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA87400.25995742527934274No Hit
CAATAATATGTTGCAATGTCTTCCGGCTGCAGGCTGTTGATGGTTAAAGT71950.21400385296165567No Hit
GTGTGATGATGTCCCCCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCA67350.20032188321011135No Hit
TCATCACACTGCAGACAATAATATGTTGCAATGTCTTCCGGCTGCAGGCT64440.19166655017163436No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT59890.1781332974826068No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG52430.15594471175510224No Hit
TTCATACTCTAAGTATCAAAATCTTCCAATTATCATGCTCACCTGAAAGA47320.1407458279658867No Hit
ATAGGAGACAGAGTCACCATCACTTGCCAGGCGAGTCAGGACATCAACAC47100.1400914728908128No Hit
CACTTTACTTTAACCATCAACAGCCTGCAGCCGGAAGACATTGCAACATA46290.1376822564780409No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC46180.13735507894050394No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT46060.13699815799046366No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA45610.13565970442781258No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG45280.13467817181520178No Hit
GGCGTAGAGTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC44620.13271510658998023No Hit
GCATGGACATGAGGGTCCCTGCTCAGCTCCTGGGGCTCCTGCTGCTCTGG44450.13220946857742313No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44050.13101973207728884No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA44010.13090075842727542No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG43310.1288187195520404No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC43220.1285510288395102No Hit
GTCCCTGGTTTGTGCTGAAACCAATTCAAATAGGTGTTGATGTCCTGACT42970.12780744352692627No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG42720.12706385821434232No Hit
GTGATGATGTCCCCCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAA41690.12400028672649654No Hit
AACCAGGGACAGCCCCTAAGCTCCTGATCTACGATGCATCCACTTTGGAG41260.12272131998885218No Hit
ACCAGGGACAGCCCCTAAGCTCCTGATCTACGATGCATCCACTTTGGAGA41130.12233465562630852No Hit
CTCTTAGGAATACAGTTTCTAGCATTAAACAAATAAACAAGGGGAGAAAA38530.11460136837543562No Hit
TAACAGACTTGGAAGCAGATGATACAGACTTCTTTTTTTCATAATCAGGT36570.1087716595247776No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC36380.1082065346872138No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT35980.10701679818707953No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35870.10668962064954259No Hit
GGACATAACAGACTTGGAAGCAGATGATACAGACTTCTTTTTTTCATAAT35560.10576757486193851No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT35270.10490501589934115No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA34940.10392348328673036No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA34780.10344758868667664No Hit
CTTCTTAATTATATGTTCATACTCTAAGTATCAAAATCTTCCAATTATCA34620.10297169408662293No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC34500.10261477313658264No Hit
GTGTAAGAAATTGCCATTTGAAACAATCCATTTTGTAACTGAACCTTATG34310.10204964829901884No Hit
TTATTACACCTCCTGATTTTCAGTCCTTGAGTTTTATTTTCTCCCCTTGT33850.10068145132386443No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)