FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001320329

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001320329
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22.4390243902439024No Hit
TATATATGGTGGGAACACAAGAAATATTTGTTGGATGCATTTACTTGGAA11.2195121951219512No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC11.2195121951219512No Hit
GCCCAGCCCTTGAGGTTTTATTTAAATCTTTCTTAAAGGCATCTTGGGCA11.2195121951219512No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11.2195121951219512No Hit
GCCATACGCAGTAGTTGAGTGTGGTCGCGAAGGCCAGCCAGGCCAGGTAG11.2195121951219512No Hit
TGCTGGTACTTTTCAAACATCTGGGCGATGCCACGGTTGGTAAGGATGTA11.2195121951219512No Hit
CAATTAGGGAGATAGTTGGTATTAGGATTAGGATTGTTGTGCAGTATAGT11.2195121951219512No Hit
GATCTCTGCTCAGTGTCACCTCTGCCTCCCAGGTTCACACCATTCTCCTG11.2195121951219512No Hit
GTGTTTGTAGTATTCTCTGATGGTAGTTTGACCCTCAGAAATAACGCCGC11.2195121951219512No Hit
GTTCATTTGTATAAATCTGACATTTGTTAATAGCTGTAATTTGAAAATGA11.2195121951219512No Hit
ATTTATTCATTCTCCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGA11.2195121951219512No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11.2195121951219512No Hit
GTTAGAACCCAGTCACACTCCACAGATTCAAGAATCCCAACAATTCACAA11.2195121951219512No Hit
ATCCTGTTATAATCTCATGTTTTTACCCTATGGCATTTACCCATGTATTT11.2195121951219512No Hit
GTGTTCTTCCGCCACGGATTGCCTCGAAGTGCTGCCGGTGGGCTGCAATA11.2195121951219512No Hit
CATGAAGAGTAGGCAATGGAAAGGAGGCCGTTTATGTGTGGCTGGAGCCA11.2195121951219512No Hit
CACATGCACTCTCTAAGCCTCTCAGCATCTCCACAAAGTAATCCATTTTG11.2195121951219512No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA11.2195121951219512No Hit
GTACTCTTTTTTTTTTTTTTTTTTTTTTTGGAACAGTCTCGTTTTGTCGC11.2195121951219512No Hit
GCGTGAACGCGACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGG11.2195121951219512No Hit
TATCAACGCAGAGTACATGGGAGAGTTCAAATATGGCATGGCCCAGATGC11.2195121951219512No Hit
CTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCGGGGCG11.2195121951219512No Hit
CACCAGCTCCGCGACATCCGCAGGTAAATCATCACGATGTAAAAGTTCAC11.2195121951219512No Hit
GCTAGAGAGAGGCAGTGGCGGCACAACTTCGTCAATGTACTAAATGCCCC11.2195121951219512No Hit
GAAATGTGTCTTGTGCATAGTGGCTCTCAACACGTTTATGTAATCCAGGA11.2195121951219512No Hit
CACTTGAGGCCAGGAGTTTGAGACCAGCCTGGGCGATGTAGTGAGACCCC11.2195121951219512No Hit
GATTTGAGGTTACATGATATGCTTTATGCTCATAACTGATGTGGCTGGAG11.2195121951219512No Hit
CTTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGC11.2195121951219512No Hit
CATATAAAGGAATATTCAGTCTAAAAGGAGAAAATCCTGCCATATGCAAC11.2195121951219512No Hit
GTGCTGCCGGTGGGCTGCAATAAAGGCGCTGCATTGACGGTGCTGACCCA11.2195121951219512No Hit
GTCATCAAGACACCATCACACGGATGGCGCCTACTTCGGCACTGGTTTCC11.2195121951219512No Hit
GGGATGAGCAAAAAAAAAAAAAAAAAAAAAAATTACTCGGCGTTGATACC11.2195121951219512No Hit
GAGATCAGGGGAGTGATGCGACCTGATTTACATAGCTGAGCAGCTGAGGC11.2195121951219512No Hit
GTATTAATGGGAGCATCCTTCAGATTACACCAATTAACTTGCATATAAAT11.2195121951219512No Hit
TGACGACGGTTGGTTTACCGGGAACGAGATCCCTGCGCTGTTGCAGGCAT11.2195121951219512No Hit
CATCTCTACCAAAAAAAAAAAAAAAACTAGCTGGACGTGGTGGCACGCGC11.2195121951219512No Hit
ACATAAAATATCAGTTGTACAAAATGAGCAGAATTGGAGAGAAGTGATCT11.2195121951219512No Hit
GTACATGGGGTGATCAGGACTCCTCAGTTCACCTTCTCACAATGAGGCTC11.2195121951219512No Hit
CTCCAAGTGTCCTTTAAAGAGACTCACCCTCTTAAATAAGGTATTCCTGC11.2195121951219512No Hit
ATATTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTACCGGCAACGGA11.2195121951219512No Hit
GAGTACATGGGGATCAAGGTTGCAAATTACCTCGTCTTTTTAAAATCAAG11.2195121951219512No Hit
ATTTTAGTACCATGAAAGAAAAGATCAAGAACAATGACTATCAGTCCATA11.2195121951219512No Hit
CTATAATTGGCTTCTTCCATTTGTCTTAGTCATCCGTATTGTATAGTGTT11.2195121951219512No Hit
GTTTAAAAATAACTAGAAAAACTCAGGCCCATCCATTTTCTCAGATCTCC11.2195121951219512No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTAAA11.2195121951219512Clontech SMART CDS Primer II A (100% over 21bp)
ACCAAGATCTGCTTCTGTGGCGATCACGACGATCTTACACGCTTGCAGAT11.2195121951219512No Hit
CAGCTATACGAAGCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCAC11.2195121951219512No Hit
TGTCGGGGTTGAGGGATAAGAGGAGAATGGGGGATAGGTGTATGAACATG11.2195121951219512No Hit
ATATATATATTGCCACTATTTATTGTGAAGGTATTTGAGCGGCTTCCTCT11.2195121951219512No Hit
ATGCATGCTGGCTCGGGTATCCCATTGCTGATACAGCACCAGCTCCGCGA11.2195121951219512No Hit
CTATTTTCTCTGTGCTCCATATATTTTGGTATGTTGTGGTTTTGTTTTCA11.2195121951219512No Hit
TTTACTTGTGTCCATGTGCCAGGGAAATCGTGATGAGAACCAGAGTATAA11.2195121951219512No Hit
ATACAGACCACAGAGTACTACATAATAAATTAGAGCCACAAGAGGCTAGG11.2195121951219512No Hit
GGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTC11.2195121951219512No Hit
ATGAAGTTCTTTGGTTTTATTTTCCCAAACAAAGAAACCTCTGGGTTATG11.2195121951219512No Hit
TAGCTCAACTTTAAATTTGCCCACAGAACCCTCTAAATCCCCTTGTAAAT11.2195121951219512No Hit
GGTTTATCGTTGCGCGATTGCACGGCCTTTGGTGATGCGATGAACGATCG11.2195121951219512No Hit
CAGCAGCACCCTGACGCTGAGCAAAGCAGACTACGAGAAACACAAACTCT11.2195121951219512No Hit
TCTCCATAATATTCATCCCTGTAGCATTGTTCGTTACATGGTCCATCATA11.2195121951219512No Hit
GTATAATACGCCTCACACTCATTCTCAACCCCCTGACAAAACACATAGCC11.2195121951219512No Hit
GGGTTCCCTTTTCTCCACATCCATTACATTCATAGTGGTTCTCTCTTGGA11.2195121951219512No Hit
TGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCAC11.2195121951219512No Hit
ATATTGTGCCTATTGCCATACTAGTCTTTGCCGCCTGCGAAGCAGCGGTG11.2195121951219512No Hit
CTTCTATATACAATTCAGATTCAACTATTCTAATTCCACCAAAAAACACC11.2195121951219512No Hit
GGATATACCTATGGTTACTTTGACTACTGGGGCCAGGGAACCCTGGTCGC11.2195121951219512No Hit
ACCTTGAAATCCATGACGCAGGGAGAATTGCGTCATTTAAAGCCTAGTTA11.2195121951219512No Hit
AGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTGGAAAAC11.2195121951219512RNA PCR Primer, Index 17 (96% over 28bp)
CCCCTAAGGGTAACAGTAAATAAGGAAAAGCGGAAGCATTTGTATTGATC11.2195121951219512No Hit
TTCCTAACTAGGACTTCTGCCTACATCTATTACATCAGAAATTAGGGCTT11.2195121951219512No Hit
TCACACTTTCGACACGATACTCTCTCAAAAAAGTATCAGTCCCAGTTTTA11.2195121951219512No Hit
GGTCTCCCCTCAGTTCTCAGTATTGAACCTTAGGTCCAAACCCATAGCAT11.2195121951219512No Hit
ATGCTGGGCCGGCACAGTGGCTCATGCCTATAATCCCAGCACTTGGGAGG11.2195121951219512No Hit
TATCAACGCAGAGTACATGGGGGAATTCGTGGAGAAAGAAATGGCTCCCC11.2195121951219512No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11.2195121951219512No Hit
CAATAAACCAAATTAGGAAAAACATAAAGAGAACCACACACAGCACATAG11.2195121951219512No Hit
CTCTTAATGGAAGACAGATACAGAAAATAGTTAACCAGAATTATCATACC11.2195121951219512No Hit
CCCATATACTCCTCCCTTTCCACGCCCCCGTCTGAGCAGGTTCCTATCTG11.2195121951219512No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTCTCCCC11.2195121951219512No Hit
GTATCAACGCAGAGTACATGGGATGATCATGCATAGGCAAACTCTGCCAA11.2195121951219512No Hit
CAAAGTGCCAGTGTTCAGAATAAGCCAGTCTCAGTAGGATGTGGCATGTT11.2195121951219512No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)