FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001320331

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001320331
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences105
Sequences flagged as poor quality0
Sequence length100
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32.857142857142857No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21.9047619047619049No Hit
GGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATG10.9523809523809524No Hit
GCATTTTATTTTAGGAAGATGGGACTGAATAAATGTGTGTTTCAATAAAT10.9523809523809524No Hit
GTGGTAGGGTGGGGGGCATCCATATAGTCACTCCAGGTTTATGGAGGGTT10.9523809523809524No Hit
GGACATCGGTGGTGCTGACCAGGAATTCTTTGTGGACATTGGCCCAGTCT10.9523809523809524No Hit
CCCCTGGAGAGCCGGCCTCCATCTCCTGCAGGTCTAGTCAGAGCCTCCTG10.9523809523809524No Hit
CTGCTATCCTAGTTGAAATCAAACATTACTTGCAAATGTGTTTTCTGAGT10.9523809523809524No Hit
ATTTCTCAGGAGGGCACATTCCACAATCATGCGTTACTAGGAGTTGGACT10.9523809523809524No Hit
GCAGAGAATTAACTGAAACATTGCGAGATGAGTATTCCTTCCCCTGATAG10.9523809523809524No Hit
ACATTGATAAGATTCATTTAAAATTCTGGAGGATTAAGAATTTTTAAACT10.9523809523809524No Hit
GTCATAAAATGTCCTTGGTCTTGGTGCACAATTGGGGCCTTTGTTGTTGC10.9523809523809524No Hit
GGCTCTGACTAGACCTGCAGGAGATGGAGGCCGGCTCTCCAGGGGTGACG10.9523809523809524No Hit
CTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTA10.9523809523809524No Hit
AGCCAGAGCTATGTGATGTGCTTGTTGACCCAGCTAGCAAAGAGTTTTGG10.9523809523809524No Hit
CTGCTCTGTTTGGCCGCCAGTCTTTTGTCTCTCTCTTCAGCAATGGTGAG10.9523809523809524No Hit
GTGATAAAAAAAAAAAAAAAAAAAAAAAAAAAAACACTCTCGTGTTTGCA10.9523809523809524No Hit
CCTAAGCTCCTGATCTACGATGCATCCACTTTGGAGACAGGGGTCCCATC10.9523809523809524No Hit
TCCCTGATCTTGTCTAGCTTTTCATGTCCCCTAGAGAAATTTCATCCACT10.9523809523809524No Hit
AAGATGTCGGGGCCGCCATCAACTGGGGTAATGAAGCCATGGCCCTTGGA10.9523809523809524No Hit
GTCATGGGCCATGGGTCACTCATATTTAGCTCGGAATAAATCTCTTCAAA10.9523809523809524No Hit
GGTCCTATTTTCAAATACGAAATGGTAGCATAAGCTGTAAAACTGTAGTC10.9523809523809524No Hit
CCACAGCTCCTGATCTATTTCGGTTCCATTCGGGCCTCCGGCGTCCCTGA10.9523809523809524No Hit
GGATAAACAAGCCCATGAGCGTATCGGCTCCTGGATGCGTCAGGCGGTCT10.9523809523809524No Hit
CGATAAACCTAAATGTTGGGTCAGCACCGTCAATGCAGCGCCTTTATTGT10.9523809523809524No Hit
CCTAGAAGGTTGCCTGGCTGGCCCAGCTCGGCTCGAATAAGGCGGCTTAG10.9523809523809524No Hit
CGGATGATCAGCCCATCTTTGATGAGCTTCCGGATCTGCTGACGGGAGTT10.9523809523809524No Hit
CTCCACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCA10.9523809523809524No Hit
CTGCAAATGACCAATGACATCAGGTAAACATGGATTGAAACAACAAGAAG10.9523809523809524No Hit
GTTCTACACAGCAAGAGAAACTATCAACACAGTAAATAGACAATGTACAG10.9523809523809524No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTATTTTT10.9523809523809524No Hit
CTGCATCTGTTCTGCCCATCTCCTTTCACTTCTACAAGCCATTTCTATAA10.9523809523809524No Hit
GGTGTGGGTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCG10.9523809523809524No Hit
ATACAACTTAATCTAAAACACTTTCTCTTTCATAAAATAGTTCCTCATTC10.9523809523809524No Hit
TTTGGGTATATACCCAGTAACGGGATTGCTAGGTTGAATGGTAGTTCTGT10.9523809523809524No Hit
CCTACACCCCTTATCCCCATACTAGTTATTATCGAAACCATCAGCCTACT10.9523809523809524No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC10.9523809523809524No Hit
ATATAAATGAATGATGCATGCATAAATGAATAAATGATTTTCCCCTAGTG10.9523809523809524No Hit
GCTTACTGACTCCCATCTTTTCTATTAGTAAGTTGGCTGTCAGACTTAAA10.9523809523809524No Hit
GCATATATAAGACACTTGTGGTCAAATTTCAAGATTGGTAAAGCCAGTTT10.9523809523809524No Hit
GTGCCAGGCCCAACATTATGTTTTTGAGGTCTGTTATGATGCCTATAGAT10.9523809523809524No Hit
TTCATGAAATGGCTGTAATATCTTACTAAATTTACAATCATTGCACATTG10.9523809523809524No Hit
GTTAAATATGCAAGCTGTACTAATGAAGGTGCTCCTTGAAGTTGATTAAG10.9523809523809524No Hit
CAGTCCTGCTGGCAGAGAGGGCAGCGATTGTTCTGTTTCACCCACAGGGA10.9523809523809524No Hit
TCTCATAGCTTCCATGATAAGGTTCAAATTCCTTGACATAGTACACAAAT10.9523809523809524No Hit
GGCAGCCCTGCCTCAGCTTGCTCATATTCTTGTTCACCTTGGGCCCTGGT10.9523809523809524No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.9523809523809524No Hit
CCTTGTAGATTCTATATATTAGAACTGTGTCGTCCAGGCACAGTGGCTCT10.9523809523809524No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAG10.9523809523809524No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAGGGTCT10.9523809523809524No Hit
CAGTAACAGCATGAGTACGAATGCCCTTACACAGCACAGATTCTAAACAG10.9523809523809524No Hit
GGTAGGAACAATGAAAAGATTCTTTTAGTGATCATAGTGGCTCCAAAACA10.9523809523809524No Hit
GGGTTAAAGCCTTAAGTGAACAAATCTAGCTAACAGTGAATGAACTAGGT10.9523809523809524No Hit
GGCGATTTGTGGGCCATACCAAGGATGTGCTGAGTGTGGCCTTCTCCTCT10.9523809523809524No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTG10.9523809523809524No Hit
CTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCG10.9523809523809524No Hit
ACCCGAGCCAGCATGCATATCTTCAATGACGACGGTTGGTTTACCGGGAA10.9523809523809524No Hit
GTCATGTACTGATGGACACAAGTTGATTATCTTGGCTATTGTGAATAGTG10.9523809523809524No Hit
CCGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTTCGAGGCAA10.9523809523809524No Hit
GAATATTCTTATTTTCTTTGGATCAAAGCTGGACTGGAAATTGTATCGTG10.9523809523809524No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCAACCCC10.9523809523809524No Hit
GAAAATACTCATTTCACATCTTTAAGCAGTTATAGCTCAATCAGAGAATT10.9523809523809524No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTACACCCGAAAAA10.9523809523809524No Hit
AAAGTACACAAGTCCAAGTCTAAAACTTTAGTACTTTTCCATGCAGATTT10.9523809523809524No Hit
GTGTAAATGAGTGAGGCAGGAGTCCGAGGAGGTTAGTTGTGGCAATAAAA10.9523809523809524No Hit
TTAAAACACTGAACTGACAATTAACAGCCCAATATCTACAATCAACCAAC10.9523809523809524No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTCTT10.9523809523809524No Hit
AATCACCGGTAAATGCGGGAGCTCCGCGCGCAGTTGCGGCATCGCATTGC10.9523809523809524No Hit
GTACATGGGAGAGTGGAGGCTGAGGACGTTGGGGTTTTTTACTGCATGCA10.9523809523809524No Hit
CCAAAGGATGGGAGGGGGTCAGGCTGGAACTGAGGAGCAGGTGGGGGCAC10.9523809523809524No Hit
AGCCTGGGTGACAGAGTGAGACTCCGTCTCAAAAAAAAAAGAGTAGCGTG10.9523809523809524No Hit
TGCCACTACACCCAGCTAATTTTGTATTTTTAGTAGGCATGAGGTTTCAC10.9523809523809524No Hit
TTGGTGATGCGATGAACGATCGCGAAATGTTAGTCAGCGTCGGTAGCGGA10.9523809523809524No Hit
GATGATGTGGAGTCCAGTGAAGAGGAAGAAGAGGAGGATGAGAAGTTGGA10.9523809523809524No Hit
CTCCATGACATACAATTGGGTCAAATGCCAATACCAGAAAAACCGGTGAC10.9523809523809524No Hit
TATGAATCTATTTAATTGCTCAGACTGTGCTAGAGAATACGTACCATGAA10.9523809523809524No Hit
GCCTCGAAGTGCTGCCGGTGGGCTGCAATAAAGGCGCTGCATTGACGGTG10.9523809523809524No Hit
ATTATGGGCAATGCGATGCCGCAACTGCGCGCGGAGTTCCCGCATTTACC10.9523809523809524No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA10.9523809523809524No Hit
GTATCAACGCAGAGTACATGGGAGCACACCCGTCTATGTAGCAAAATAGT10.9523809523809524No Hit
ACGAGAAACACAAACTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGC10.9523809523809524No Hit
CTCTATACAAGAATTCGTTACCTTGCCTTGAAAAGCATTGTTACAAGACA10.9523809523809524No Hit
GCATATGCCTGTTGGTTCATACCATTGTGCATATTTGGAACATTACTTCC10.9523809523809524No Hit
CAAAAGCAGGCCTTACAATATTGATTTCATTATATGCATTATTTGCCAAA10.9523809523809524No Hit
CTGCAGGTTCTGTGCTGGTGTTCCTTTGAACCAGTCGCAGGTCTCGCTCA10.9523809523809524No Hit
CTATAGCGGATTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCG10.9523809523809524No Hit
CTAAGAATGTCATGCCCGGGCACGGTGGCTCACACCTGTAATCCCAGCAA10.9523809523809524No Hit
ACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACGGG10.9523809523809524No Hit
GTGCTACAGAGACCTGCTACACTTATGACAGAAACAAGTGCTACACAGCT10.9523809523809524No Hit
GACACCAGACATATGATTACAGCCCACCCTCATGACCTCATTTAACCTTA10.9523809523809524No Hit
GTGTTTATTTTCCTGAAACACTTATCTACTTGTTTGTTGCCTTGTGTATT10.9523809523809524No Hit
GTAGCGGATTTATTATGGGCAATGCGATGCCGCAACTGCGCGCGGAGCTC10.9523809523809524No Hit
GCGCAAGCAGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTAT10.9523809523809524No Hit
ATTTACAAGGGGATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTA10.9523809523809524No Hit
CTTGTTGGTTGATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTT10.9523809523809524No Hit
CGCTGGATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTG10.9523809523809524No Hit
AAGTATATGTTTTCAAATTGCCATTGCTACTATTGCTTGTCGGTGTTATT10.9523809523809524No Hit
CTTCCGAAGATCCTAATGAGGACATTGTGGAGAGAAACATCCGAATTATT10.9523809523809524No Hit
ACCATTTCCGGCGGCATCTACGGCTCAACATTTTTTGTAGCCACAGGCTT10.9523809523809524No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTCGAAAAAAGGG10.9523809523809524No Hit
TCCTTTTTCTTTGTCTTTCTTGTATAAGTATCCTGGATAGAACCTCCAGT10.9523809523809524No Hit
GGTAAGACCCACTGCTGTGCTCACTTCAGGTCTGACCCGGCATAATCTCA10.9523809523809524No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)