FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001320332

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001320332
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences105
Sequences flagged as poor quality0
Sequence length100
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACACTAAAGGACGAACCTGATCTCTTATACTAGTATCCTTAATCATTTTT10.9523809523809524No Hit
GTTAATGGTTGATATTGCTAGGGTGGCGCTTCCAATTAGGTGCATGAGTA10.9523809523809524No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTTACCCTAG10.9523809523809524No Hit
TGAGCCCACCATCTTGCCCAGGAGCGTCACAACCCCCCGACCCCGCGCTC10.9523809523809524No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA10.9523809523809524No Hit
CAAGTAATCCTTCTGCTCTAGCCTCCTCAGTAGCTGGGACTACATGTGTG10.9523809523809524No Hit
GTGTTACATCACCCAAAAAAAAAAAAAAAGCCCTGTTTTAAATTTCATTG10.9523809523809524No Hit
ATTTTAAATGTTCGTGAGAAGCTTAACTACTTTTGTATCTAGCCTTTTTT10.9523809523809524No Hit
CGACATTCACGTCAGTTTTGCTACGGGGAGTGATGGGATGGAGATGAAGG10.9523809523809524No Hit
TTTTGTGTCTGCTGGTATACAGACCAGTTTTAGGACTGGTAATCCAACAG10.9523809523809524No Hit
TTCGGCGCTCGTTCACCTTTGTGATTGTCCGCCACTGATTGCCTCGACGT10.9523809523809524No Hit
CATTTAAGCCAAATTTTCTTAACCCAGAGATTTTTTTTTTTTTTTTTTTT10.9523809523809524No Hit
GACATCGATTATCTGATAACGAAAACCGCTATAGACAAATGCCTGCAACA10.9523809523809524No Hit
GTCACCGGTAAATGCGGGAGCTCCGCGCGCAGTTGCGGCATCGCATTGCC10.9523809523809524No Hit
CAAATTACAAATGTGTGTGCGTGGGACGAAGACATCTTTGAAGGTCATGA10.9523809523809524No Hit
CCACATGATATCTCAGATGCAGGAAAAGCACTTAACAAAACTGAACACAC10.9523809523809524No Hit
CTTATTTCTGCAATGGAGAAAGACAATTTCATACCAAAAAAAAAAAAAAA10.9523809523809524No Hit
CACAATCCTAGCACCCAAGGGCGAGCAAGCGCCCAACAAATTACCCCTGT10.9523809523809524No Hit
CTCTTTCCCGGTAAACCAACCGTCGTCATTGAAGATATGCATGCTGGCTC10.9523809523809524No Hit
AAGAATTACCGCGCATATAGTTGTTTCTTTTATAAATTTCTTTTCCGTAA10.9523809523809524No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC10.9523809523809524No Hit
CCTTCTCACAATGAGGCTCCCTGCTCAGCTCCTGGGGCTGCTAATTCTCT10.9523809523809524No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC10.9523809523809524No Hit
ATACGAAGCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATT10.9523809523809524No Hit
CTATACCTATTATTCGGCGCATGAGCTGGAGTCCTAGGCACAGCTCTAAG10.9523809523809524No Hit
CTCCACCTCCTATTCTTGCACGAAACGGGATCAAACAACCCCCTAGGAAT10.9523809523809524No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGCAAACA10.9523809523809524No Hit
GCGGGAAGATGAAGACAGAAAGCGCACCCACCGTTCGCTTGAATTCCACC10.9523809523809524No Hit
GCCCTGGCTTCTGCAGGAACCAATCCAAACGGTTGTATCCATTACTATGC10.9523809523809524No Hit
CCAACAGAGAGAGGCACCCTGGATCTTTTGACGTGGTTCACGTGAAAGAT10.9523809523809524No Hit
CCCTAGACCAAACCTACGCCAAAATCCACTTCACTATCATATTCATCGGC10.9523809523809524No Hit
TTATTAATGTGTTACAATTCAGTATCATGTAGGTATTCATATTCAATAAG10.9523809523809524No Hit
AAGAAGACCACACTACGTAACAACAAAGTTCCAAGAAATATAGAAAACTT10.9523809523809524No Hit
CTATAGACTTTACTCACTCTCCATGTTCTTCGTTTAAACGACGCCTGAAT10.9523809523809524No Hit
TCCTCGTACAGCTGCTCCCATGAGAAAAAGTTCAACAGAAAACATATTAA10.9523809523809524No Hit
GGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTCCTGGGAG10.9523809523809524No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG10.9523809523809524No Hit
GTCTGGGCAACATGGTGAGACCCTGCATCTACAAAAAATAAATTAGCCAG10.9523809523809524No Hit
CTCTAGAAGGCACTGATAGTTTAAACCACAGTAGGCTGAAAATGATCAGG10.9523809523809524No Hit
GCCTTACTCCTGTGCACAAGGTGTTTCCAGAGTAGTTTTAGAAACATTTT10.9523809523809524No Hit
TGTTGGCTGTATGTATGTCTTCTTTTGAAGTGTCTGTTCATGTCCTTTGC10.9523809523809524No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC10.9523809523809524No Hit
GCATTGACGGAGCTGACCCAACATTTAGGTTTATCGTTGCGCGATTGCAC10.9523809523809524No Hit
GGTTAGTGTAAGAAATTGCCATTTGAAACAATCCATTTTGTAACTGAACC10.9523809523809524No Hit
CAATAAAGGCGCTGCATTGACGGTGCTGACCCAACATTTAGGTTTATCGT10.9523809523809524No Hit
TCTGGAAGGCGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGG10.9523809523809524No Hit
ATATAGACCAGCAATTTCATTTCTAGTGTATACCCTAGAGAAATGTCCAT10.9523809523809524No Hit
ATTATGGGCAATGCGATGCCGCAACTGCGCGCGGAGCTCCCGCATTTACC10.9523809523809524No Hit
GCATGAAGTCAATACGTGTCACAGATTCTGAATGCTTTCAGGAGTAGCAC10.9523809523809524No Hit
GGATCGTTGACCTCGTCTGTTATGTAAAGGATGCGTAGGGATGGGAGGGC10.9523809523809524No Hit
GATCACTTGAAAAAGAAGATTCTTCCTTCTATGATTCAAGGGAGCCTAGC10.9523809523809524No Hit
CGCTCACCATCCCCTCTGCGCGGCAACGTGGTCCCAAGCCCACTGCCCAC10.9523809523809524No Hit
CACTAATACCACTCTTCTCTACCATCCTCCACTCATATCCACACTTTAAG10.9523809523809524No Hit
GATCGCCAACCGCCCAGGTATTGACGGAGAAACCGAACTTGATCTGTGCG10.9523809523809524No Hit
CCACCACACCCGGATAATTTTGTATTTTTAGTAGAGATGGGGTTTCTCCA10.9523809523809524No Hit
ATCTGATAACGAAAACCGCTATAGACAAATGCCTGCAACAACGCAGGGAT10.9523809523809524No Hit
CTTATAAGTCAATGGCAACCAGATGCTTGAGAATAAAGCAAGCAAGGGAA10.9523809523809524No Hit
GAGAAGTATGGGGGACCTTTAACCAAAGGAAATTAAAAATATGCAAGTTA10.9523809523809524No Hit
TGTGTGACATGATTGCAACTTAGCAAAGAGAGTATGCATGAGAGCAAGGG10.9523809523809524No Hit
GTGTAAGATCGTCGTGATCGCCACAGAAGCAGATCTTGGTGACGCTGCCG10.9523809523809524No Hit
AGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACAGAAGCAGAT10.9523809523809524No Hit
GTACTTGGGGAGCCCGTGAGCTTCTGACGCAGCATTTCGAGCGTGTACAC10.9523809523809524No Hit
GGTCCAGAACTATTCTTGGAGCTGCCACATAGAGGAAGAATTTCGGCAGT10.9523809523809524No Hit
ATCCTATATCCAGAAAAAAATACCTTTCAAAAATGAAGGTGATTCATTGG10.9523809523809524No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC10.9523809523809524No Hit
GCTCACTGCAGCCTCTGCCTCTCAGGCTCATGTGATCCTCACACCTCTGC10.9523809523809524No Hit
GCTATACGAAGCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGG10.9523809523809524No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT10.9523809523809524No Hit
GGACTACACATGCTGTAGGGATGATGCAGATAATCTTCAGGGGATAAGTG10.9523809523809524No Hit
ATCAGGGCCTGAGCTCGCCCGTCACAAAGAGCTTCAACAGGGGAGAGTGT10.9523809523809524No Hit
GTACATGGGTCAGACCAGCTTTGGAGCTCTTGGAACCAATTGAACAGAAA10.9523809523809524No Hit
TATTTACCCTATAGCACCCCCTCTAGAGCCAAAAAAAAAAAAAAAAAAAA10.9523809523809524No Hit
GTATCAACGCAGAGTACATGGGAAGCATAATATAGCAAGGACTAACCCCT10.9523809523809524No Hit
GCTGGGACGTGGAGTGCGATACGTGCGCCATCTGCAGGGTCCAGGTGATG10.9523809523809524No Hit
ATTCTAAACCTTATTTAGACATTGGTACCAGTTACCCAGGTGAAAATATG10.9523809523809524No Hit
TTCTTCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTT10.9523809523809524No Hit
ATACAAAATAAGAGCATTCAAAACAGAACAATTTAAGTCTGACAGCCAAC10.9523809523809524No Hit
GTTCTTAAGGACCGATGGAGGTTAATTAGACAAAGTACAGTGAATTTAAG10.9523809523809524No Hit
GAGTTAATGTGAAATTGTAAATGGAAATTTTTCCTTAAAATACAACACAA10.9523809523809524No Hit
GAATAGGATTACCTTAAGGTAACCTTGGCTAGGCACGGTGGCTCACACCT10.9523809523809524No Hit
GTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACAGAAGC10.9523809523809524No Hit
GTGATGACCTGGGGACTTGGAACGGGATCTGTGCCGCCGATCTCGGGACC10.9523809523809524No Hit
ACGCAGAGTACATGGGCGAGATTGCACCATTGTACTCCAGCCTGGGCAAC10.9523809523809524No Hit
GGTTAACACTACAAACTGTAAAGTCTGAAAATAGTTGCATTAGATGTTTT10.9523809523809524No Hit
GTACATGGGGCTCTTTCCTTTCGCTGCTGCGGCCGCAGCCATGAGTATGC10.9523809523809524No Hit
CTTATAAACAACAGAAATGTATTTCTCATAGTTGTAGAGGCTGGGAAATC10.9523809523809524No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTCG10.9523809523809524No Hit
GAATGCACGCGCGTTCCGTTGCCGGTAATCAAATACGCATCCAGCGATAG10.9523809523809524No Hit
GTCAAACCACATCTACAAAATGCCAGTATCAGGCGGCGGCTTCGAAGCCA10.9523809523809524No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.9523809523809524No Hit
GTATACGCACTCACATACTCCCTCTCGAAATCCGTACTCCCCACAACAAT10.9523809523809524No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT10.9523809523809524No Hit
AAGCAAGACAGCTTGACTCCAGTTGATTGCAAATCTAATATGATTCCAGA10.9523809523809524No Hit
GCCTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAG10.9523809523809524No Hit
TACCGGGTTTTCCTCTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.9523809523809524No Hit
CAGAATAGACAGTCAATACATACTTGTTTATTAAATAAGTGAATCTATGA10.9523809523809524No Hit
CTAAAGGGGTTATTGTTTAGAGAAAGTGGAAATGTCAGACAAAATAACAG10.9523809523809524No Hit
GCTTTCAGGGAGAGTAACTTGAACTAAAAATTGTTTAAGCAGGAGAGGGA10.9523809523809524No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.9523809523809524No Hit
CCAAAGGCCGTGCAATCTCCAAACGAAAACCCTACATGTGGGGACAGCAC10.9523809523809524No Hit
ATTTTTACATTTACTATAGCATCTAACATGGTTAGCAAATGCTTTTATTA10.9523809523809524No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA10.9523809523809524No Hit
ACATTTTCCATAACAGGTGTAAGAGTGTTGAAAAAAAAATTCAAATTTTT10.9523809523809524No Hit
CTTCCAAAGTCAAAAACAATAAAATAAAATAACACCGACAAGCAATAGTA10.9523809523809524No Hit
GGTATATATTCAAAGGAAAGGAAATCAGTATGTCAAAGAGACATCTACAT10.9523809523809524No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)