FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001320404

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001320404
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2633434
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT575542.1855113893114466Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT368551.3995034620195532Clontech Universal Primer Mix Long (96% over 25bp)
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT309671.175917072537227Clontech Universal Primer Mix Long (96% over 25bp)
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA232000.8809789802972088No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC230720.8761184066128105Clontech Universal Primer Mix Long (96% over 25bp)
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA190690.7241115592796327No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA189010.7177320563188597No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT174250.6616835660206407Clontech SMARTer II A Oligonucleotide (95% over 24bp)
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT172040.6532914817686716Clontech Universal Primer Mix Long (96% over 25bp)
ATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGTTCCGTTGCCGGTAA160830.6107234887982763No Hit
GTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACAGAAGC154760.5876737370292934No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC141110.5358402754730136Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CCTTTATTGCAGCCCACCGGCAGCACTTCGAGGCAATCCGTGGCGGAAAA130180.4943355329960804No Hit
GTGTAAGATCGTCGTGATCGCCACAGAAGCAGATCTTGGTGACGCTGCCG130160.49425958653226165No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTG126590.48070314274061926No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC125300.4758045958243115Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC124240.4717794332419191No Hit
CGCCTAATGCTTCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGA123480.4688934676168076No Hit
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCG121830.4626278843517627No Hit
GGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTT120300.4568179798696303No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCA116800.44352734870135346No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC112120.42575587616777183Clontech Universal Primer Mix Long (96% over 25bp)
GCTATAGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACC110960.4213509812662858No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA106070.4027820708626075Clontech Universal Primer Mix Long (96% over 25bp)
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG101770.3864535811415817Clontech Universal Primer Mix Long (96% over 25bp)
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA96920.3680365636655409Clontech Universal Primer Mix Long (96% over 25bp)
TGCTGATACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATCACGATG95420.3623405788791365No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC92390.3508346896105997Clontech Universal Primer Mix Long (96% over 25bp)
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTT90120.34221476596717443No Hit
ACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCAT85640.32520275807178006No Hit
GGTAAATCATCACGATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGT85130.3232661232444025No Hit
CCGGTAAATGCGGGAGCTCCGCGCGCAGTTGCGGCATCGCATTGCCCATA83560.31730432583463264No Hit
GCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATTGCCTCGA79730.3027605780133468No Hit
CCTAAATGTTGGGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCAC79000.29998853208396337No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC78890.2995708265329604Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CTTCGAGGCAATCCGTGGCGGAAAAACACAAATGTGCACGCTCGCCTAAT78650.2986594689671357No Hit
ACCTAAATGTTGGGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCA75150.28536883779885885No Hit
TCCCATTGCTGATACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATC74890.28438153376921543No Hit
AGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACCAACCG74730.2837739620586656No Hit
CCTAATGCTTCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATC72740.27621728890870245No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72460.27515403841524033No Hit
ATACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATCACGATGTAAAA71220.2704453576584794No Hit
GTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCG71070.2698757591798389No Hit
TCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACAGAA70850.26904034807783295No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA70360.2671796597142742Clontech Universal Primer Mix Long (96% over 25bp)
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG70350.26714168648236486Clontech Universal Primer Mix Long (96% over 25bp)
CCACAGAAGCAGATCTTGGTGACGCTGCCGAGTGGCATTTTTTTGACATC68910.26167354108741664No Hit
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC68590.26045839766631707Clontech Universal Primer Mix Long (96% over 25bp)
GCATATTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTACCGGCAACG68430.25985082595576725No Hit
GAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCAT68360.25958501333240175No Hit
CTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCA67310.25559782398191866No Hit
TCGATTATCTGATAACGAAAACCGCTATAGACAAATGCCTGCAACAACGC66460.25237009926962284No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT65610.24914237455732707Clontech Universal Primer Mix Long (96% over 25bp)
ACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACGGG64630.24542099783020954No Hit
GGATGGCACTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCT63880.24257300543700736No Hit
CGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTTCGAGGCAAT62560.23756053882497152No Hit
ATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACAGAAGCAGATCTTGGT61670.23418092118503825No Hit
CCGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTTCGAGGCAA60520.22981399951546155No Hit
GCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACCAACCGTCGTCATT58220.2210801561763082No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG57370.21785243146401237Clontech Universal Primer Mix Long (96% over 25bp)
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC56530.21466267998362593Clontech Universal Primer Mix Long (96% over 25bp)
AGAATATGCTGCATCTCCAGCGCATGACGCCCCGTGGCAAAAGTGAGGGT56120.21310577747534207No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55030.20896669519722158No Hit
GCCTAATGCTTCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGAT53980.20497950584673852No Hit
GATCTTGGTGACGCTGCCGAGTGGCATTTTTTTGACATCGATTATCTGAT53870.2045618002957355No Hit
CTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTA53420.20285300485981422No Hit
GCCTTTGGTGATGCGATGAACGATCGCGAAATGTTAGTCAGCGTCGGTAG52130.19795445794350647No Hit
TGCGGGAGCTCCGCGCGCAGTTGCGGCATCGCATTGCCCATAATAAATCC51710.19635958220331323No Hit
GCTTCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACA50730.19263820547619573No Hit
CAGTTGCGGCATCGCATTGCCCATAATAAATCCGCTACCGACGCTGACTA49940.1896383201553561No Hit
GCATTGACGGTGCTGACCCAACATTTAGGTTTATCGTTGCGCGATTGCAC49510.1880054711832535No Hit
GAAGATATGCATGCTGGCTCGGGTATCCCATTGCTGATACAGCACCAGCT49160.18667640806642583No Hit
AATGTGCACGCTCGCCTAATGCTTCGTATAGCTGGATCTGCAAGCGTGTA48250.18322084396267382No Hit
GCACTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACT47600.18075258388856527No Hit
GATATGGATGGCACTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAAC47270.17949946723555632No Hit
CCGCTATAGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAA46980.1783982435101848No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT46460.17642363545089795Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GGTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTC46000.1746768667830673No Hit
ACACAAATGTGCACGCTCGCCTAATGCTTCGTATAGCTGGATCTGCAAGC45510.17281617841950853No Hit
CAACAACGCAGGGATCTCTTTCCCGGTAAACCAACCGTCGTCATTGAAGA45290.17198076731750256No Hit
CTATAGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACCA45280.17194279408559318No Hit
ACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCT44470.16886696230093481No Hit
GTGCTGACCCAACATTTAGGTTTATCGTTGCGCGATTGCACGGCCTTTGG43640.16571518405245775No Hit
CGGGAGCTCCGCGCGCAGTTGCGGCATCGCATTGCCCATAATAAATCCGC43380.16472788002281433No Hit
CGTGTAAGATCGTCGTGATCGCCACAGAAGCAGATCTTGGTGACGCTGCC43180.1639684153846271No Hit
GGCTCGGGTATCCCATTGCTGATACAGCACCAGCTCCGCGACATCCGCAG42820.16260137903589003No Hit
GGGTATCCCATTGCTGATACAGCACCAGCTCCGCGACATCCGCAGGTAAA42650.16195583409343087No Hit
CAGCTATACGAAGCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCAC41790.1586901361492257No Hit
GCTGATACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATCACGATGT41660.158196484134404No Hit
AGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACAGAAGCAGAT41460.15743701949621675No Hit
GTGCACGCTCGCCTAATGCTTCGTATAGCTGGATCTGCAAGCGTGTAAGA41410.15724715333666991No Hit
ATGCATGCTGGCTCGGGTATCCCATTGCTGATACAGCACCAGCTCCGCGA41360.1570572871771231No Hit
TCCAGCGATAGCGCCCCGAGAATATGCTGCATCTCCAGCGCATGACGCCC41350.15701931394521373No Hit
ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG41010.1557282240602954Clontech Universal Primer Mix Long (96% over 25bp)
GATCTCTTTCCCGGTAAACCAACCGTCGTCATTGAAGATATGCATGCTGG40600.15417132155201155No Hit
GAAGCAGATCTTGGTGACGCTGCCGAGTGGCATTTTTTTGACATCGATTA39570.1502600786653472No Hit
ACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGC39090.14843736353369782No Hit
CTTCTGTGGCGATCACGACGATCTTACACGCTTGCAGATCCAGCTATACG38820.14741208627214505No Hit
GCATTTGATATGGATGGCACTTTATTGGTGCCCGACCATCATTTAGGTGA38750.1471462736487795No Hit
CCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCAC38620.14665262163395779No Hit
ATGCACGCGCGTTCCGTTGCCGGTAATCAAATACGCATCCAGCGATAGCG38590.1465387019382297No Hit
TCATTGAAGATATGCATGCTGGCTCGGGTATCCCATTGCTGATACAGCAC38530.14631086254677353No Hit
CTATCGCTGGATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTC38510.1462349160829548No Hit
CTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCACATTTGTGTT38010.1443362544874867No Hit
ACCAAGATCTGCTTCTGTGGCGATCACGACGATCTTACACGCTTGCAGAT37870.1438046292407556No Hit
CCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTT37770.143424896921662No Hit
ATCAAATACGCATCCAGCGATAGCGCCCCGAGAATATGCTGCATCTCCAG37560.14262745905156538No Hit
GCACCAGCTCCGCGACATCCGCAGGTAAATCATCACGATGTAAAAGTTCA36870.14000730604981937No Hit
GCATTGCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATC36320.13791877829480442No Hit
TATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGT35960.13655174194606737No Hit
CGATTATCTGATAACGAAAACCGCTATAGACAAATGCCTGCAACAACGCA35860.13617200962697376No Hit
GAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCT35800.13594417023551758No Hit
ATACGAAGCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATT35790.13590619700360823No Hit
GTTGCGGCATCGCATTGCCCATAATAAATCCGCTACCGACGCTGACTAAC35730.13567835761215202No Hit
CCTTCCAGAGAATGCACGCGCGTTCCGTTGCCGGTAATCAAATACGCATC35040.13305820461040604No Hit
CTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCGGGGCG34850.13233671320412815No Hit
ATTATGGGCAATGCGATGCCGCAACTGCGCGCGGAGCTCCCGCATTTACC34530.13112156978302855No Hit
CGATAAACCTAAATGTTGGGTCAGCACCGTCAATGCAGCGCCTTTATTGC34120.1295646672747447No Hit
ACGATAAACCTAAATGTTGGGTCAGCACCGTCAATGCAGCGCCTTTATTG34090.1294507475790166No Hit
GGTATCCCATTGCTGATACAGCACCAGCTCCGCGACATCCGCAGGTAAAT33460.12705843396872676No Hit
GGTAAATGCGGGAGCTCCGCGCGCAGTTGCGGCATCGCATTGCCCATAAT33410.12686856780917996No Hit
GTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACG33390.12679262134536123No Hit
CTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCG33370.1267166748815425No Hit
GATCTGCTTCTGTGGCGATCACGACGATCTTACACGCTTGCAGATCCAGC33120.12576734408380846No Hit
GGGTAATGTCGCGTTCACGCAGTCGCGCCAAAGTAGAGAGGGTTTTCTCA33090.12565342438808036No Hit
CATTTGTGTTTTTCCGCCACGGATTGCCTCGAAGTGCTGCCGGTGGGCTG32880.12485598651798376No Hit
GAATGCACGCGCGTTCCGTTGCCGGTAATCAAATACGCATCCAGCGATAG32820.12462814712652756No Hit
GGGATCTCTTTCCCGGTAAACCAACCGTCGTCATTGAAGATATGCATGCT32530.12352692340115605No Hit
TATGCATGCTGGCTCGGGTATCCCATTGCTGATACAGCACCAGCTCCGCG32290.12261556583533136No Hit
ATAGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACCAAC32160.12212191382050964No Hit
GTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACT31860.12098271686322877No Hit
CTGCAAGCGTGTAAGATCGTCGTGATCGCCACAGAAGCAGATCTTGGTGA31360.11908405526776064No Hit
AATCATCACGATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGTTCCG31210.11851445678912022No Hit
GCTCCGCGACATCCGCAGGTAAATCATCACGATGTAAAAGTTCACCTTCC30960.11756512599138615No Hit
CATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATTGCCTCGAA30430.11555254470018994No Hit
ATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGC30330.1151728123810963No Hit
GGAGATGCAGCATATTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTA30250.11486902652582143No Hit
TTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTACCGGCAACGGAACG30100.11429942804718099No Hit
GTCATGCGCTGGAGATGCAGCATATTCTCGGGGCGCTATCGCTGGATGCG29970.11380577603235928No Hit
CTTCCAGAGAATGCACGCGCGTTCCGTTGCCGGTAATCAAATACGCATCC29830.1132741507856282No Hit
CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT29710.11281847200271586Clontech Universal Primer Mix Long (96% over 25bp)
GATATGCATGCTGGCTCGGGTATCCCATTGCTGATACAGCACCAGCTCCG29320.11133751595825071No Hit
CTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACG28930.10985655991378558No Hit
CACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGC28840.10951480082660131No Hit
CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT28770.10924898820323578Clontech Universal Primer Mix Long (96% over 25bp)
AAGCAGATCTTGGTGACGCTGCCGAGTGGCATTTTTTTGACATCGATTAT28630.1087173629565047No Hit
ATCGCTGGATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTC28340.10761613923113318No Hit
ACGCAGGGATCTCTTTCCCGGTAAACCAACCGTCGTCATTGAAGATATGC28290.10742627307158638No Hit
GTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAAC28210.10712248721631147No Hit
TGTTGGGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAG28110.10674275489721786No Hit
GCCTCGAAGTGCTGCCGGTGGGCTGCAATAAAGGCGCTGCATTGACGGTG27970.10621112965048679No Hit
GCTATACGAAGCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGG27880.10586937056330252No Hit
CTTTCCCGGTAAACCAACCGTCGTCATTGAAGATATGCATGCTGGCTCGG27870.10583139733139316No Hit
CAATAAAGGCGCTGCATTGACGGTGCTGACCCAACATTTAGGTTTATCGT27760.10541369178039017No Hit
GTCTGGCAGCATTTGATATGGATGGCACTTTATTGGTGCCCGACCATCAT27730.10529977208466208No Hit
CACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCGGGG27730.10529977208466208No Hit
GTTCATCGCATCACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAAT27510.1044643609826561No Hit
ATGCGGGAGCTCCGCGCGCAGTTGCGGCATCGCATTGCCCATAATAAATC27000.10252772615527861No Hit
GCCGAGTGGCATTTTTTTGACATCGATTATCTGATAACGAAAACCGCTAT26710.10142650242990711No Hit
CTCTTTCCCGGTAAACCAACCGTCGTCATTGAAGATATGCATGCTGGCTC26600.10100879687890411No Hit
CAGCGATAGCGCCCCGAGAATATGCTGCATCTCCAGCGCATGACGCCCCG26590.10097082364699475No Hit
GACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACCAACCGT26420.1003252787045356No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)