FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001320482

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001320482
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3566505
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA110950.31108886711220085No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA99900.2801061543443792No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92390.2590491251238958No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA89880.25201142294767565No Hit
GGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTTAATTGTC82520.23137497353852018No Hit
ATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGTTCCGTTGCCGGTAA79700.22346807308555577No Hit
GTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACAGAAGC79440.22273906807925406No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64410.1805969709841988No Hit
CCTTTATTGCAGCCCACCGGCAGCACTTCGAGGCAATCCGTGGCGGAAAA63200.1772042938394871No Hit
GTGTAAGATCGTCGTGATCGCCACAGAAGCAGATCTTGGTGACGCTGCCG62860.1762509796004772No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC60800.17047501685824076No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC59400.16654960528584706No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACA57970.16254007775118778No Hit
CGCCTAATGCTTCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGA56900.15953994176371547No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCA56790.15923151656874165No Hit
GGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTT56500.1584183956001744No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTG54660.15325928324788554No Hit
GCTATAGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACC53870.15104422957489194No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATAT53010.14863290532327866No Hit
ATTCTATACCAACACCTATTCTGATTTTTCGGCCACCCTGAAGTTTATAT51450.14425887528546855No Hit
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCG49080.1376137142664878No Hit
TGCTGATACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATCACGATG48690.13652020675703525No Hit
ATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTGACGC46580.13060405074435616No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCT46070.12917407938584133No Hit
GTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTGAGCTTGAACGCTTT43140.12095875373790306No Hit
GGTAAATCATCACGATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGT42780.11994936219071613No Hit
GTGTTGGGTTGACAGTGAGGGTAATAATGACTTGTTGGTTGATTGTAGAT41270.11571552542334862No Hit
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTT40630.11392105156168293No Hit
CCTAAATGTTGGGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCAC39870.11179011385095493No Hit
GTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCT39700.11131345673144999No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT39690.11128541807736145Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTATGCGGAGGAG39000.10935075094525312No Hit
CCGGTAAATGCGGGAGCTCCGCGCGCAGTTGCGGCATCGCATTGCCCATA38490.10792077958673829No Hit
GTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGA37180.10424771590114132No Hit
TCCCATTGCTGATACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATC37160.10419163859296425No Hit
ACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATA36930.10354674954892815No Hit
GCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATTGCCTCGA36920.10351871089483962No Hit
TACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTG36570.10253735800174119No Hit
AGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACCAACCG36510.10236912607721005No Hit
ACCTAAATGTTGGGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCA36400.10206070088223625No Hit
ATACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATCACGATGTAAAA36030.10102327068096079No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)