FastQCFastQC Report
Fri 9 Sep 2022
EGAF00001320498

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001320498
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2930103
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA285050.9728326956424399No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA237770.8114731802943446No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA231910.7914738833413024No Hit
ATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGTTCCGTTGCCGGTAA217700.7429772946548295No Hit
GTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACAGAAGC206810.705811365675541No Hit
GTGTAAGATCGTCGTGATCGCCACAGAAGCAGATCTTGGTGACGCTGCCG176120.6010710203702737No Hit
CGCCTAATGCTTCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGA159640.544827263751479No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC157320.5369094533536876No Hit
CCTTTATTGCAGCCCACCGGCAGCACTTCGAGGCAATCCGTGGCGGAAAA156860.5353395426713669No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTG156530.5342133023992672No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCA151770.5179681396865571No Hit
GCTATAGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACC147530.5034976586147313No Hit
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCG146160.4988220550608631No Hit
GGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTT144150.49196222794898337No Hit
TGCTGATACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATCACGATG122360.41759624149731256No Hit
GGTAAATCATCACGATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGT121280.41391036424316824No Hit
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTT119380.40742595055532177No Hit
ACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCAT111320.3799183851216152No Hit
CTTCGAGGCAATCCGTGGCGGAAAAACACAAATGTGCACGCTCGCCTAAT108370.3698504796589062No Hit
AGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACCAACCG103260.35241081968790855No Hit
TCCCATTGCTGATACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATC101490.34637007641028317No Hit
CCTAAATGTTGGGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCAC98290.335448958620226No Hit
CCGGTAAATGCGGGAGCTCCGCGCGCAGTTGCGGCATCGCATTGCCCATA98020.3345274893066899No Hit
CCTAATGCTTCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATC97700.3334353775276842No Hit
GCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATTGCCTCGA97010.3310805115042031No Hit
ATACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATCACGATGTAAAA96680.32995427123210347No Hit
TCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACAGAA96360.3288621594530977No Hit
ACCTAAATGTTGGGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCA92230.31476709180530515No Hit
TCGATTATCTGATAACGAAAACCGCTATAGACAAATGCCTGCAACAACGC92130.31442580687436583No Hit
GTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCG91550.31244635427491796No Hit
CCACAGAAGCAGATCTTGGTGACGCTGCCGAGTGGCATTTTTTTGACATC89250.30459680086331437No Hit
GAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCAT88420.3017641359365183No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85910.29319788416994214No Hit
GGATGGCACTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCT84950.289921548832925No Hit
GCATATTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTACCGGCAACG84560.2885905376022618No Hit
ATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACAGAAGCAGATCTTGGT81330.27756703433292274No Hit
ACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACGGG80340.2741883135166238No Hit
CTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCA77420.2642227935331966No Hit
AGAATATGCTGCATCTCCAGCGCATGACGCCCCGTGGCAAAAGTGAGGGT77080.263062424768003No Hit
GCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACCAACCGTCGTCATT75890.2590011340898255No Hit
CGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTTCGAGGCAAT75670.25825030724175907No Hit
CCGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTTCGAGGCAA74280.25350644670170297No Hit
GATCTTGGTGACGCTGCCGAGTGGCATTTTTTTGACATCGATTATCTGAT73440.250639653281813No Hit
ACACAAATGTGCACGCTCGCCTAATGCTTCGTATAGCTGGATCTGCAAGC72740.24825065876523797No Hit
CTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTA72100.24606643520722649No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69520.2372612839889929No Hit
GCCTAATGCTTCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGAT68640.23425797659672715No Hit
CCGCTATAGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAA66540.22709099304700212No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT65470.22343924428595172Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCTTCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACA63520.21678418813263564No Hit
AATGTGCACGCTCGCCTAATGCTTCGTATAGCTGGATCTGCAAGCGTGTA63370.21627226073622668No Hit
GAAGATATGCATGCTGGCTCGGGTATCCCATTGCTGATACAGCACCAGCT62920.21473647854699987No Hit
CGTGTAAGATCGTCGTGATCGCCACAGAAGCAGATCTTGGTGACGCTGCC62750.2141562941644031No Hit
CAGTTGCGGCATCGCATTGCCCATAATAAATCCGCTACCGACGCTGACTA61850.21108472978594953No Hit
GGTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTC61640.21036803143097701No Hit
TGCGGGAGCTCCGCGCGCAGTTGCGGCATCGCATTGCCCATAATAAATCC60420.20620435527351771No Hit
CAACAACGCAGGGATCTCTTTCCCGGTAAACCAACCGTCGTCATTGAAGA59930.20453205911191516No Hit
CTATAGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACCA59410.2027573774710309No Hit
GATCTCTTTCCCGGTAAACCAACCGTCGTCATTGAAGATATGCATGCTGG58860.2008803103508648No Hit
GATATGGATGGCACTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAAC58830.200777924871583No Hit
GGGTATCCCATTGCTGATACAGCACCAGCTCCGCGACATCCGCAGGTAAA58810.20070966788539515No Hit
GCCTTTGGTGATGCGATGAACGATCGCGAAATGTTAGTCAGCGTCGGTAG58500.19965168459948335No Hit
GCACTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACT58330.19907150021688658No Hit
GGCTCGGGTATCCCATTGCTGATACAGCACCAGCTCCGCGACATCCGCAG58080.19821828788953835No Hit
AGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACAGAAGCAGAT56550.19299662844616725No Hit
ACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCT55500.18941313667130474No Hit
GTGCACGCTCGCCTAATGCTTCGTATAGCTGGATCTGCAAGCGTGTAAGA55160.18825276790611115No Hit
GCATTGACGGTGCTGACCCAACATTTAGGTTTATCGTTGCGCGATTGCAC54680.18661460023760257No Hit
ATGCACGCGCGTTCCGTTGCCGGTAATCAAATACGCATCCAGCGATAGCG54680.18661460023760257No Hit
ATGCATGCTGGCTCGGGTATCCCATTGCTGATACAGCACCAGCTCCGCGA54410.1856931309240665No Hit
TCCAGCGATAGCGCCCCGAGAATATGCTGCATCTCCAGCGCATGACGCCC54260.18518120352765757No Hit
CAGCTATACGAAGCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCAC53540.1827239520248947No Hit
GCATTTGATATGGATGGCACTTTATTGGTGCCCGACCATCATTTAGGTGA53400.18224615312157968No Hit
GCTGATACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATCACGATGT52840.18033495750831965No Hit
CTATCGCTGGATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTC52320.1785602758674354No Hit
GTGCTGACCCAACATTTAGGTTTATCGTTGCGCGATTGCACGGCCTTTGG51350.1752498120373243No Hit
GCACCAGCTCCGCGACATCCGCAGGTAAATCATCACGATGTAAAAGTTCA51090.17436247121688214No Hit
GAAGCAGATCTTGGTGACGCTGCCGAGTGGCATTTTTTTGACATCGATTA50980.17398705779284893No Hit
ACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGC50790.17333861642406428No Hit
TCATTGAAGATATGCATGCTGGCTCGGGTATCCCATTGCTGATACAGCAC50740.17316797395859465No Hit
CGGGAGCTCCGCGCGCAGTTGCGGCATCGCATTGCCCATAATAAATCCGC50240.17146154930389818No Hit
ACCAAGATCTGCTTCTGTGGCGATCACGACGATCTTACACGCTTGCAGAT50150.17115439286605283No Hit
ATCAAATACGCATCCAGCGATAGCGCCCCGAGAATATGCTGCATCTCCAG49640.16941383971826246No Hit
CTTCTGTGGCGATCACGACGATCTTACACGCTTGCAGATCCAGCTATACG49350.16842411341853852No Hit
CTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCACATTTGTGTT48520.16559144849174245No Hit
CCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCAC48210.16453346520583065No Hit
CGATTATCTGATAACGAAAACCGCTATAGACAAATGCCTGCAACAACGCA47910.1635096104130128No Hit
CCTTCCAGAGAATGCACGCGCGTTCCGTTGCCGGTAATCAAATACGCATC46660.15924354877627167No Hit
GGTATCCCATTGCTGATACAGCACCAGCTCCGCGACATCCGCAGGTAAAT46640.15917529179008383No Hit
TATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGT46570.15893639233842632No Hit
GGGATCTCTTTCCCGGTAAACCAACCGTCGTCATTGAAGATATGCATGCT46530.1587998783660506No Hit
ATACGAAGCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATT46360.1582196939834538No Hit
GCATTGCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATC46210.15770776658704488No Hit
CCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTT46100.1573323531630117No Hit
GAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCT45980.15692281124588453No Hit
GGGTAATGTCGCGTTCACGCAGTCGCGCCAAAGTAGAGAGGGTTTTCTCA45610.15566005700140917No Hit
TATGCATGCTGGCTCGGGTATCCCATTGCTGATACAGCACCAGCTCCGCG44750.15272500659533128No Hit
GAATGCACGCGCGTTCCGTTGCCGGTAATCAAATACGCATCCAGCGATAG44740.15269087810223736No Hit
CTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCGGGGCG44460.15173528029560737No Hit
GATCTGCTTCTGTGGCGATCACGACGATCTTACACGCTTGCAGATCCAGC44200.1508479394751652No Hit
ATTATGGGCAATGCGATGCCGCAACTGCGCGCGGAGCTCCCGCATTTACC44110.15054078303731985No Hit
CTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCG43800.14948279975140805No Hit
GTTGCGGCATCGCATTGCCCATAATAAATCCGCTACCGACGCTGACTAAC43440.1482541740000266No Hit
CGATAAACCTAAATGTTGGGTCAGCACCGTCAATGCAGCGCCTTTATTGC43080.14702554824864517No Hit
AATCATCACGATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGTTCCG42580.14531912359394875No Hit
GCTCCGCGACATCCGCAGGTAAATCATCACGATGTAAAAGTTCACCTTCC41980.14327141400831303No Hit
ACGATAAACCTAAATGTTGGGTCAGCACCGTCAATGCAGCGCCTTTATTG41980.14327141400831303No Hit
GATATGCATGCTGGCTCGGGTATCCCATTGCTGATACAGCACCAGCTCCG41890.14296425757046766No Hit
ATAGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACCAAC41570.14187214579146193No Hit
TTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTACCGGCAACGGAACG41100.1402681066160473No Hit
CTGCAAGCGTGTAAGATCGTCGTGATCGCCACAGAAGCAGATCTTGGTGA40440.13801562607184797No Hit
CATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATTGCCTCGAA39930.1362750729240576No Hit
GGTAAATGCGGGAGCTCCGCGCGCAGTTGCGGCATCGCATTGCCCATAAT39830.1359337879931183No Hit
GGAGATGCAGCATATTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTA39080.13337415101107367No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT38950.13293048060085258Clontech Universal Primer Mix Long (96% over 25bp)
CTTTCCCGGTAAACCAACCGTCGTCATTGAAGATATGCATGCTGGCTCGG38710.1321113967665983No Hit
CATTTGTGTTTTTCCGCCACGGATTGCCTCGAAGTGCTGCCGGTGGGCTG38670.1319748827942226No Hit
CTTCCAGAGAATGCACGCGCGTTCCGTTGCCGGTAATCAAATACGCATCC38310.13074625704284115No Hit
ATCGCTGGATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTC38110.1300636871809626No Hit
ACGCAGGGATCTCTTTCCCGGTAAACCAACCGTCGTCATTGAAGATATGC38050.129858916222399No Hit
ATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGC37620.12839139101936006No Hit
CAGCGATAGCGCCCCGAGAATATGCTGCATCTCCAGCGCATGACGCCCCG37450.1278112066367633No Hit
GTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACG37430.12774294965057542No Hit
GACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACCAACCGT36960.12613891047516076No Hit
GTCATGCGCTGGAGATGCAGCATATTCTCGGGGCGCTATCGCTGGATGCG36940.12607065348897292No Hit
GCTATACGAAGCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGG36900.1259341395165972No Hit
AAGCAGATCTTGGTGACGCTGCCGAGTGGCATTTTTTTGACATCGATTAT36630.12501267020306112No Hit
CACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCGGGG36530.12467138527212182No Hit
GTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAAC36190.12351101650692825No Hit
GCCGAGTGGCATTTTTTTGACATCGATTATCTGATAACGAAAACCGCTAT35910.12255541870029825No Hit
GTCTGGCAGCATTTGATATGGATGGCACTTTATTGGTGCCCGACCATCAT35690.12180459185223182No Hit
GCGTGTAAGATCGTCGTGATCGCCACAGAAGCAGATCTTGGTGACGCTGC35450.12098550801797753No Hit
CTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACG35250.12030293815609894No Hit
ATGCGGGAGCTCCGCGCGCAGTTGCGGCATCGCATTGCCCATAATAAATC35190.12009816719753537No Hit
CACCAGCTCCGCGACATCCGCAGGTAAATCATCACGATGTAAAAGTTCAC35050.11962036829422037No Hit
CTCTTTCCCGGTAAACCAACCGTCGTCATTGAAGATATGCATGCTGGCTC35030.11955211130803252No Hit
GTTCATCGCATCACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAAT34740.11856238500830857No Hit
TGTTGGGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAG34660.11828935706355716No Hit
CAATAAAGGCGCTGCATTGACGGTGCTGACCCAACATTTAGGTTTATCGT34210.11675357487433036No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT34200.11671944638123642Clontech Universal Primer Mix Long (96% over 25bp)
TGGTGACGCTGCCGAGTGGCATTTTTTTGACATCGATTATCTGATAACGA34170.11661706090195464No Hit
CCCCGAGAATATGCTGCATCTCCAGCGCATGACGCCCCGTGGCAAAAGTG33880.11562733460223071No Hit
CACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGC33820.11542256364366714No Hit
AGATATGCATGCTGGCTCGGGTATCCCATTGCTGATACAGCACCAGCTCC33740.1151495356989157No Hit
TACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATCACGATGTAAAAG33650.11484237926107034No Hit
GATTATCTGATAACGAAAACCGCTATAGACAAATGCCTGCAACAACGCAG33130.11306769762018604No Hit
TCACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAG33030.11272641268924677No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32790.11190732885499248No Hit
TTCTTATACAGGTTATAGCGTTTAAGATGATTCGCCGGATCGCTGGCCCG32090.10951833433841746No Hit
GTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACT32080.10948420584532352No Hit
GCATCGCATTGCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCG32050.10938182036604174No Hit
CATTTGATATGGATGGCACTTTATTGGTGCCCGACCATCATTTAGGTGAG31910.10890402146272675No Hit
GCCTCGAAGTGCTGCCGGTGGGCTGCAATAAAGGCGCTGCATTGACGGTG31710.10822145160084816No Hit
TTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGC31630.10794842365609673No Hit
CTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCACA31480.1074364962596878No Hit
GGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGG31430.10726585379421814No Hit
ATGTTGGGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCA30850.1052864011947703No Hit
GTAAACCAACCGTCGTCATTGAAGATATGCATGCTGGCTCGGGTATCCCA30730.10487685927764315No Hit
ATCTGATAACGAAAACCGCTATAGACAAATGCCTGCAACAACGCAGGGAT30560.10429667489504635No Hit
GCAATAAAGGCGCTGCATTGACGGTGCTGACCCAACATTTAGGTTTATCG30540.10422841790885849No Hit
TCTCTTTCCCGGTAAACCAACCGTCGTCATTGAAGATATGCATGCTGGCT30410.10378474749863742No Hit
GTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACT30250.10323869160913456No Hit
GGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTTAATTGTC30090.1026926357196317No Hit
GCGCATGACGCCCCGTGGCAAAAGTGAGGGTAATGTCGCGTTCACGCAGT30050.10255612174725598No Hit
AGGCAATCCGTGGCGGAAAAACACAAATGTGCACGCTCGCCTAATGCTTC29990.10235135078869241No Hit
CTCGCCTAATGCTTCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGT29590.10098621106493526No Hit
AGATCGTCGTGATCGCCACAGAAGCAGATCTTGGTGACGCTGCCGAGTGG29580.10095208257184132No Hit
TCGCATCACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGG29370.10023538421686881No Hit
ATCTGCTTCTGTGGCGATCACGACGATCTTACACGCTTGCAGATCCAGCT29370.10023538421686881No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)