FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001320613

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001320613
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1646421
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT1042936.334528045985809Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT483952.9394061421714133Clontech Universal Primer Mix Long (96% over 25bp)
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT365282.218630593268672Clontech Universal Primer Mix Long (96% over 25bp)
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC334072.029067899401186Clontech Universal Primer Mix Long (96% over 25bp)
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT290011.7614571242713741Clontech SMARTer II A Oligonucleotide (95% over 24bp)
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC269821.6388274930895559Clontech SMARTer II A Oligonucleotide (95% over 24bp)
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT255631.5526405457656334Clontech Universal Primer Mix Long (96% over 25bp)
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC231801.407902353043359Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA195541.187667066928811Clontech Universal Primer Mix Long (96% over 25bp)
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG183261.1130810406329852Clontech Universal Primer Mix Long (96% over 25bp)
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA175751.067466948004186Clontech Universal Primer Mix Long (96% over 25bp)
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC171831.0436577278836945Clontech Universal Primer Mix Long (96% over 25bp)
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC161620.9816444275188425Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA138670.8422511617623925Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC138230.8395786982794801Clontech Universal Primer Mix Long (96% over 25bp)
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC114420.6949619811700652Clontech Universal Primer Mix Long (96% over 25bp)
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG105950.6435170591240029Clontech Universal Primer Mix Long (96% over 25bp)
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA105360.6399335285446432Clontech Universal Primer Mix Long (96% over 25bp)
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA103740.6300940039030115Clontech Universal Primer Mix Long (96% over 25bp)
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT99460.6040982227510461Clontech Universal Primer Mix Long (96% over 25bp)
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG98640.5991177226238004Clontech Universal Primer Mix Long (96% over 25bp)
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC91740.5572086361872206Clontech Universal Primer Mix Long (96% over 25bp)
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT85300.5180934888464129Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTG80550.48924303079224574No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT79860.4850521221485877Clontech SMART CDS Primer II A (96% over 26bp)
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCG78370.4760021889905437No Hit
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTT70480.42808005971741125No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA70150.42607571210522704No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCA68890.41842274849506905No Hit
ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG67070.40736846772484075Clontech Universal Primer Mix Long (96% over 25bp)
ATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGTTCCGTTGCCGGTAA62550.37991497921855955No Hit
ACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCAT59850.3635157714825066No Hit
GCTATAGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACC54520.3311425206554095No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC54040.3282271059467779No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG52850.32099930698162865Clontech SMART CDS Primer II A (96% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT52680.3199667642723216No Hit
AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG51920.31535069098365487Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCAT51500.31279970311360217No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA49270.29925517227975107Clontech SMART CDS Primer II A (96% over 26bp)
GCATATTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTACCGGCAACG48790.2963397575711194No Hit
AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA48440.2942139343460755Clontech SMARTer II A Oligonucleotide (95% over 24bp)
ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT48220.2928777026046193TruSeq Adapter, Index 3 (95% over 21bp)
CTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTA48050.2918451598953123No Hit
GTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCG46770.28407072067229466No Hit
ACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACGGG46620.2831596535758472No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA45540.27659997048142604No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC45110.27398824480494355Clontech SMART CDS Primer II A (96% over 26bp)
GTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACT44010.26730708609766274No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT43440.2638450311311627Clontech SMART CDS Primer II A (96% over 26bp)
GCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATTGCCTCGA42780.25983633590679417No Hit
CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT42140.2559491162952854Clontech Universal Primer Mix Long (96% over 25bp)
GGATGGCACTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCT41520.2521833722966361No Hit
CTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCA40510.24604885384722375No Hit
GTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACG39470.23973212197852192No Hit
CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT39030.23705965849560956Clontech Universal Primer Mix Long (96% over 25bp)
TGCTGATACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATCACGATG38100.23141104249763578No Hit
AGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACCAACCG37550.22807046314399537No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA36960.22448693256463567No Hit
AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG36720.22302922521031987Clontech SMARTer II A Oligonucleotide (95% over 24bp)
ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA35090.21312896276225826Clontech Universal Primer Mix Long (96% over 25bp)
AGAATATGCTGCATCTCCAGCGCATGACGCCCCGTGGCAAAAGTGAGGGT32960.20019180999270536No Hit
ATACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATCACGATGTAAAA31880.1936321268982842No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG31880.1936321268982842Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCCCATTGCTGATACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATC30640.18610063890098583No Hit
GGGATACCCGAGCCAGCATGCATATCTTCAATGACGACGGTTGGTTTACC30610.18591842548169635No Hit
CCGGTAAATGCGGGAGCTCCGCGCGCAGTTGCGGCATCGCATTGCCCATA30060.18257784612805594No Hit
GGTAAATCATCACGATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGT29380.1784476752908278No Hit
ACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCT29090.17668627890436286No Hit
CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA28890.17547152277576633Clontech Universal Primer Mix Long (96% over 25bp)
GGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTT28860.17528930935647688No Hit
TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA28750.17462119348574878Clontech Universal Primer Mix Long (96% over 25bp)
TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG28610.17377086419573123Clontech Universal Primer Mix Long (96% over 25bp)
GGTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTC28470.17292053490571366No Hit
GATATGGATGGCACTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAAC28410.17255610806713473No Hit
ACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGC28240.17152356535782767No Hit
GCATTGACGGTGCTGACCCAACATTTAGGTTTATCGTTGCGCGATTGCAC27690.16818298600418727No Hit
GCACTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACT27110.16466019323125738No Hit
CTATCGCTGGATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTC26570.16138035168404677No Hit
TATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGT26470.16077297361974854No Hit
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCTGCTTG26080.15840419916898532TruSeq Adapter, Index 3 (96% over 28bp)
ACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCT25910.1573716564596783RNA PCR Primer, Index 3 (95% over 23bp)
ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG25890.15725018084681866Clontech SMART CDS Primer II A (96% over 26bp)
CTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCGGGGCG25610.1555495222667835No Hit
CAACAACGCAGGGATCTCTTTCCCGGTAAACCAACCGTCGTCATTGAAGA24850.15093344897811678No Hit
CAGCTATACGAAGCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCAC24510.1488683635595027No Hit
GCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACCAACCGTCGTCATT24370.14801803426948515No Hit
GTGCTGACCCAACATTTAGGTTTATCGTTGCGCGATTGCACGGCCTTTGG24360.1479572964630553No Hit
CCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTT24310.1476536074309062No Hit
TCGATTATCTGATAACGAAAACCGCTATAGACAAATGCCTGCAACAACGC24300.14759286962447637No Hit
GAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCT24130.14656032691516935No Hit
CTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCG23860.14492040614156404No Hit
CTTCTGTGGCGATCACGACGATCTTACACGCTTGCAGATCCAGCTATACG23800.14455597930298508No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23490.14267310730366048No Hit
CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC23460.14249089388437103Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCT23400.14212646704579204No Hit
GAAGATATGCATGCTGGCTCGGGTATCCCATTGCTGATACAGCACCAGCT23370.1419442536265026No Hit
CTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCACATTTGTGTT23290.14145835117506397No Hit
GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT23250.14121539994934468Clontech Universal Primer Mix Long (96% over 25bp)
CCTAAATGTTGGGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCAC23140.14054728407861658No Hit
ATACGAAGCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATT22890.13902883891787093No Hit
CCGCTATAGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAA22860.13884662549858146No Hit
GTCATGCGCTGGAGATGCAGCATATTCTCGGGGCGCTATCGCTGGATGCG22840.13872514988572182No Hit
ACCAAGATCTGCTTCTGTGGCGATCACGACGATCTTACACGCTTGCAGAT22670.1376926071764148No Hit
GGAGATGCAGCATATTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTA22140.13447350343563402No Hit
TCCAGCGATAGCGCCCCGAGAATATGCTGCATCTCCAGCGCATGACGCCC22100.1342305522099147No Hit
ACCTAAATGTTGGGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCA21780.1322869424041603No Hit
GAAATGGCTCGTCTGGCAGCATTTGATATGGATGGCACTTTATTGGTGCC21730.13198325337201117No Hit
GTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGA21700.1318010399527217No Hit
GGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGG21460.13034333259840586No Hit
TGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATA21350.1296752167276778No Hit
ATCGCTGGATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTC21230.12894636305051987No Hit
ATCAAATACGCATCCAGCGATAGCGCCCCGAGAATATGCTGCATCTCCAG21160.1285211984055111No Hit
TTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTACCGGCAACGGAACG21100.12815677156693214No Hit
CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG21080.1280352959540725Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATTTGTGTTTTTCCGCCACGGATTGCCTCGAAGTGCTGCCGGTGGGCTG20830.12651685079332686No Hit
CTATAGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACCA20570.1249376678261514No Hit
GCATTTGATATGGATGGCACTTTATTGGTGCCCGACCATCATTTAGGTGA20400.12390512511684436No Hit
GCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACGGTTGGT20130.12226520434323905No Hit
CTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACG20040.12171856408537063No Hit
CACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCGGGG20030.12165782627894081No Hit
CCTTTATTGCAGCCCACCGGCAGCACTTCGAGGCAATCCGTGGCGGAAAA19950.1211719238275022No Hit
CAATAAAGGCGCTGCATTGACGGTGCTGACCCAACATTTAGGTTTATCGT19880.12074675918249342No Hit
ATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGC19860.12062528356963377No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19460.11819577131244073No Hit
GATCTGCTTCTGTGGCGATCACGACGATCTTACACGCTTGCAGATCCAGC19460.11819577131244073No Hit
ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA19410.1178920822802916Clontech Universal Primer Mix Long (96% over 25bp)
ATGCACGCGCGTTCCGTTGCCGGTAATCAAATACGCATCCAGCGATAGCG19160.11637363711954596No Hit
ATGCATGCTGGCTCGGGTATCCCATTGCTGATACAGCACCAGCTCCGCGA18920.11491592976523016No Hit
GGGTAATGTCGCGTTCACGCAGTCGCGCCAAAGTAGAGAGGGTTTTCTCA18900.11479445415237051No Hit
ACCCGAGCCAGCATGCATATCTTCAATGACGACGGTTGGTTTACCGGGAA18750.11388338705592312No Hit
GCCTTTGGTGATGCGATGAACGATCGCGAAATGTTAGTCAGCGTCGGTAG18710.11364043583020382No Hit
CATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATTGCCTCGAA18400.11175756383087923No Hit
GCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGA18300.11115018576658096No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC18080.1098139540251248Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT18020.10944952718654585Clontech Universal Primer Mix Long (96% over 28bp)
GGCTCGGGTATCCCATTGCTGATACAGCACCAGCTCCGCGACATCCGCAG17900.10872067350938795No Hit
GGGTATCCCATTGCTGATACAGCACCAGCTCCGCGACATCCGCAGGTAAA17800.10811329544508969No Hit
GATCTCTTTCCCGGTAAACCAACCGTCGTCATTGAAGATATGCATGCTGG17720.10762739299365108No Hit
GTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCA17600.10689853931649318No Hit
CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT17460.1060482100264756Clontech Universal Primer Mix Long (96% over 25bp)
GCAATAAAGGCGCTGCATTGACGGTGCTGACCCAACATTTAGGTTTATCG17250.10477271609144927No Hit
GATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATAC17190.10440828925287031No Hit
GCTGATACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATCACGATGT17130.10404386241429137No Hit
TGCGGGAGCTCCGCGCGCAGTTGCGGCATCGCATTGCCCATAATAAATCC17070.10367943557571241No Hit
GCGTGAACGCGACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGG16610.10088549647994043No Hit
ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT16590.10076402086708078Clontech SMART CDS Primer II A (96% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)