FastQCFastQC Report
Sat 10 Sep 2022
EGAF00001320637

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001320637
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2507404
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT118100.4710050713805992Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74180.2958438289162815No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCC61270.2443563143394523No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT57370.2288023788747246Clontech Universal Primer Mix Long (96% over 25bp)
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA52600.20977871934478848No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52240.2083429714557367No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT50480.20132375955370574No Hit
CCCAAATACTACGACCTGCATATGAGCAACAGTAAAAATCAGCCTCGTCC49630.19793379926011126No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAATACTACGA46280.18457336751476824No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA45630.18198104493731365No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAAGCAGCAGAGATATT43700.1742838409765638No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT43400.173087384402354No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT42800.17069447125393433Clontech Universal Primer Mix Long (96% over 25bp)
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG42330.16882002262100562No Hit
GCATATGAGCAACAGTAAAAATCAGCCTCGTCCTCAGCCTGGAGCCCAGA40760.16255856654930756No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA39340.15689533876471443No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC38430.15326608715627799No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT38010.1515910479523842No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC37800.15075352835043734Clontech Universal Primer Mix Long (96% over 25bp)
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC37540.1497165993194555No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG37100.14796179634394777No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG36970.14744333182845684No Hit
GTACATGGGGGGGGGCACAAGAGGCAGCGCTCTCGGGACGTCTCCACCAT36570.14584805639617707No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT36120.14405337153486236Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CTCCTGCACTGGAAGCAGCAGAGATATTGGGAATTATAACCTTGTCTCCT36020.14365455267679242No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35860.1430164425038805No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTC35860.1430164425038805No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA34530.1377121516915503No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT33970.13547876608635864No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCA33740.13456148271279778No Hit
GTATTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCC33330.13292632539471103No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTT32010.1276619164681878No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCA31950.12742262515334585No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC31650.12622616857913604Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCT31440.12538864897718915No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA29730.11856884650419319No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT29540.11781109067386031No Hit
CTCATATGCAGGTCGTAGTATTTGGGTGTTCGGCGGAGGGACCAAGCTGA28930.11537829563963366No Hit
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACA27970.11154963460216223No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT27500.10967518596923351Clontech Universal Primer Mix Long (96% over 25bp)
TCACTAAGCGGCCCTCAGGGGTTTCTGATCGCTTCTCTGGCTCCAAGTCT26850.10708286339177892No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG26640.10624534378983204No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC26520.1057667611601481Clontech SMARTer II A Oligonucleotide (95% over 24bp)
TTATAACCTTGTCTCCTGGTACCAACAGGACCCAGGCAAAGCCCCCAAAC26460.10552746984530614No Hit
GTTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC26180.1044107770427103No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC25920.10337384801172847No Hit
GTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCA25470.10157916315041374No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)