FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001320638

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001320638
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2507404
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT104680.41748358062761326Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66250.2642174934713353No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT61740.246230762972381Clontech Universal Primer Mix Long (96% over 25bp)
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCC58780.23442572477351079No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT48230.1923503352471321No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA46950.18724545386383687No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT46410.18509183203025917Clontech Universal Primer Mix Long (96% over 25bp)
CCCAAATACTACGACCTGCATATGAGCAACAGTAAAAATCAGCCTCGTCC45170.18014647819019194No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44700.1782720295572632No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA43690.17424395909075682No Hit
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT42340.16885990450681262No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC41310.16475207026869226Clontech Universal Primer Mix Long (96% over 25bp)
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAATACTACGA41140.16407407820997333No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAAGCAGCAGAGATATT40770.16259844843511456No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA38850.1549411263601717No Hit
GTATTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCC38510.15358514224273392No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC38270.15262797698336605No Hit
GCATATGAGCAACAGTAAAAATCAGCCTCGTCCTCAGCCTGGAGCCCAGA38180.15226904001110314No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG37360.1489987253749296No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG35890.14313608816130147No Hit
CTCCTGCACTGGAAGCAGCAGAGATATTGGGAATTATAACCTTGTCTCCT35190.14034435615481192No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTC34980.13950683655286503No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT34480.13751274226251534No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC33960.13543888420055164No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT33690.13436207328376282No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT32880.1311316405333963Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG32440.12937683755788856No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA32320.12889825492820461No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCA31680.12634581423655702No Hit
GTACATGGGGGGGGGCACAAGAGGCAGCGCTCTCGGGACGTCTCCACCAT31480.12554817652041714No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCA30150.12024388570808693No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT29350.11705333484352742Clontech Universal Primer Mix Long (96% over 25bp)
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTT29310.11689380730029943No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT29150.11625569712738752No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCT28470.11354372889251192No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28120.11214786288926715No Hit
CTCATATGCAGGTCGTAGTATTTGGGTGTTCGGCGGAGGGACCAAGCTGA27380.1091966033395496No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA27380.1091966033395496No Hit
TTATAACCTTGTCTCCTGGTACCAACAGGACCCAGGCAAAGCCCCCAAAC25690.10245656463816762No Hit
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC25680.1024166827523606No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC25460.10153928126460673Clontech SMARTer II A Oligonucleotide (95% over 24bp)
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG25380.1012202261781508No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)