FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001320639

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001320639
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2566008
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT121310.47275768430963583Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74380.2898665943364167No Hit
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCC61050.2379181982285324No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT58510.22801955410895058Clontech Universal Primer Mix Long (96% over 25bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56730.22108270901727506No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA52210.20346779900920026No Hit
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT49820.1941537204872315No Hit
CCCAAATACTACGACCTGCATATGAGCAACAGTAAAAATCAGCCTCGTCC49690.19364709696930016No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAATACTACGA47440.18487861300510364No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA47230.18406022116844528No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT43430.1692512260289134Clontech Universal Primer Mix Long (96% over 25bp)
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT43430.1692512260289134No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG42760.16664016635957488No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAAGCAGCAGAGATATT42680.16632839804084787No Hit
GCATATGAGCAACAGTAAAAATCAGCCTCGTCCTCAGCCTGGAGCCCAGA40350.15724814575792437No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC38880.151519402901316No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA38450.14984364818815843No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT38310.14929805363038617No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC38220.14894731427181832Clontech Universal Primer Mix Long (96% over 25bp)
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT37540.14629728356263894Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG37530.14625831252279806No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG37120.14466049988932225No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36140.14084133798491666No Hit
CTCCTGCACTGGAAGCAGCAGAGATATTGGGAATTATAACCTTGTCTCCT35780.1394383805506452No Hit
GTACATGGGGGGGGGCACAAGAGGCAGCGCTCTCGGGACGTCTCCACCAT35710.1391655832717591No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA35670.13900969911239558No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTC35540.13850307559446423No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC35070.1366714367219432No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT34840.1357751028056031No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC33980.132423593379288Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GTATTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCC33780.13164417258247052No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCA33660.13117652010438005No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTT31950.12451247229159068No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA31180.12151170222384342No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCA30410.11851093215609616No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCT30340.11823813487721005No Hit
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT29640.11551016208834891No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT28240.11005421651062662Clontech Universal Primer Mix Long (96% over 25bp)
CACCAGTGTGGCCTTGTTGGCTTGAAGCTCCTCAGAGGAGGGTGGGAACA28040.10927479571380914No Hit
CTCATATGCAGGTCGTAGTATTTGGGTGTTCGGCGGAGGGACCAAGCTGA27960.10896302739508218No Hit
TCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGAAGGCAG27730.10806669347874208No Hit
TTATAACCTTGTCTCCTGGTACCAACAGGACCCAGGCAAAGCCCCCAAAC27600.10756006996081073No Hit
TCACTAAGCGGCCCTCAGGGGTTTCTGATCGCTTCTCTGGCTCCAAGTCT27520.10724830164208372No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC27330.10650785188510714Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CCACCACACCCTCCAAACAAAGCAACAACAAGTACGCGGCCAGCAGCTAC26940.10498798133131307No Hit
GTTCTGTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACC26790.10440341573369996No Hit
GTAGGGGCCACTGTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCA26630.103779879096246No Hit
GTCGATCACCATCTCCTGCACTGGAAGCAGCAGAGATATTGGGAATTATA25800.1005452827894535No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)