FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001320640

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001320640
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2566008
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT107700.41971809908620705Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67040.2612618510932156No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT66040.25736474710912827Clontech Universal Primer Mix Long (96% over 25bp)
TCCTAGGTCAGCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCC57460.22392759492565883No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT49830.19419269152707241Clontech Universal Primer Mix Long (96% over 25bp)
GTTCCCACCCTCCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGT49330.19224413953502872No Hit
CCCAAATACTACGACCTGCATATGAGCAACAGTAAAAATCAGCCTCGTCC45580.1776299995947012No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45450.17712337607676984No Hit
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGA45230.1762660132002706No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA44330.172758619614592No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC43140.16812106587352804Clontech Universal Primer Mix Long (96% over 25bp)
CCTCTGAGGAGCTTCAAGCCAACAAGGCCACACTGGTGTGTCTCATAAGT42480.16554897724403042No Hit
GACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCAAATACTACGA42210.16449675916832684No Hit
CTCCTGGACAGTCGATCACCATCTCCTGCACTGGAAGCAGCAGAGATATT40880.15931361086949067No Hit
GCCTTGGGCTGACCTAGGACGGTCAGCTTGGTCCCTCCGCCGAACACCCA39700.1547150281682676No Hit
GCATATGAGCAACAGTAAAAATCAGCCTCGTCCTCAGCCTGGAGCCCAGA38750.15101277938338462No Hit
GTATTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAGGTCAGCCC38260.1491031984311818No Hit
TATTTATTGAGGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGG37990.14805098035547826No Hit
GCCCAAGGCTGCCCCCTCGGTCACTCTGTTCCCACCCTCCTCTGAGGAGC37430.14586860212438932No Hit
GTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCTGCCTTCCAG35230.13729497335939717No Hit
CTCCTGCACTGGAAGCAGCAGAGATATTGGGAATTATAACCTTGTCTCCT35140.1369442340008293No Hit
GTTTGGAGGGTGTGGTGGTCTCCACTCCCGCCTTGACGGGGCTGCTATCT35040.13655452360242057No Hit
GTCCTGGGCCCAGTCTGCCCTGACTCAGCCTGCCTCCGTGTCTGGGTCTC34810.13565818968608048No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT34730.1353464213673535No Hit
CCTCAGAGGAGGGTGGGAACAGAGTGACCGAGGGGGCAGCCTTGGGCTGA33670.1312154911442209No Hit
GTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCC32560.12688970572188396No Hit
GGGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAG32390.1262271980445891No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT32360.12611028492506648Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GTACATGGGGGGGGGCACAAGAGGCAGCGCTCTCGGGACGTCTCCACCAT31270.12186244158241127No Hit
CTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCA30830.12014771582941285No Hit
GTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCA30820.12010874478957197No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT29950.11671826432341599Clontech Universal Primer Mix Long (96% over 25bp)
GTCTTCTCCACGGTGCTCCCTTCATGCGTGACCTGGCAGCTGTAGCTTTT29790.11609472768596202No Hit
GCTCAGGCGTCAGGCTCAGGTAGCTGCTGGCCGCGTACTTGTTGTTGCTT29590.11531530688914454No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29420.1146527992118497No Hit
CCCTTCATGCGTGACCTGGCAGCTGTAGCTTTTGTGGGACTTCCACTGCT28000.10911891155444566No Hit
GGTGTGTCTCATAAGTGACTTCTACCCGGGAGCCGTGACAGTGGCCTGGA27520.10724830164208372No Hit
CTCATATGCAGGTCGTAGTATTTGGGTGTTCGGCGGAGGGACCAAGCTGA27460.10701447540303849No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC27050.10541666276956269Clontech SMARTer II A Oligonucleotide (95% over 24bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)