FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001320643

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001320643
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1537677
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT745904.8508236775343585Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT360552.344770715826536Clontech Universal Primer Mix Long (96% over 25bp)
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT280021.8210586488579852Clontech Universal Primer Mix Long (96% over 25bp)
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC261761.7023080920115212Clontech Universal Primer Mix Long (96% over 25bp)
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT220991.4371678837623245Clontech SMARTer II A Oligonucleotide (95% over 24bp)
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT188441.2254849360431352Clontech Universal Primer Mix Long (96% over 25bp)
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC187881.2218430788780739Clontech SMARTer II A Oligonucleotide (95% over 24bp)
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC163221.061471297288052Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG138530.9009044162070448Clontech Universal Primer Mix Long (96% over 25bp)
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA132880.8641606787381226Clontech Universal Primer Mix Long (96% over 25bp)
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC130250.8470569566950666Clontech Universal Primer Mix Long (96% over 25bp)
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA121680.791323535436896Clontech Universal Primer Mix Long (96% over 25bp)
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC103440.6727030449177558Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC89800.5839978096830478Clontech Universal Primer Mix Long (96% over 25bp)
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA74230.48274117386161075Clontech Universal Primer Mix Long (96% over 25bp)
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT73720.4794244825148585Clontech Universal Primer Mix Long (96% over 25bp)
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG72000.46823877836502725Clontech Universal Primer Mix Long (96% over 25bp)
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC70960.46147532934419905Clontech Universal Primer Mix Long (96% over 25bp)
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG64980.4225854974744371Clontech Universal Primer Mix Long (96% over 25bp)
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT64000.4162122474355798Clontech SMARTer II A Oligonucleotide (95% over 24bp)
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC61120.3974826963009787Clontech Universal Primer Mix Long (96% over 25bp)
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA59090.3842809640776314Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG45600.2965512262978506Clontech Universal Primer Mix Long (96% over 25bp)
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA41690.27112325930608316Clontech Universal Primer Mix Long (96% over 25bp)
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCG39450.25655583064583787No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTG39170.2547349020633072No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37800.2458253586416393No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA36500.23737104736560408No Hit
AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG35570.2313229631450558Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTT35330.22976216721717238No Hit
AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA34260.2228036187053588Clontech SMARTer II A Oligonucleotide (95% over 24bp)
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCA32740.21291857782876378No Hit
ACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCAT32600.21200811353749843No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32220.20953685331834968No Hit
GTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACG32010.2081711568814517No Hit
CTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTA31680.206025062480612No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT30210.19646518742232602Clontech SMART CDS Primer II A (96% over 26bp)
GTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACT29610.19256319760261745No Hit
CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT29220.1900269042198069Clontech Universal Primer Mix Long (96% over 25bp)
CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT29000.1885961746192471Clontech Universal Primer Mix Long (96% over 25bp)
GCATATTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTACCGGCAACG27330.17773563628772493No Hit
GAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCAT25470.1656394678466284No Hit
ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA24910.16199761068156707Clontech Universal Primer Mix Long (96% over 25bp)
GTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCG24300.1580305876981967No Hit
TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA23660.1538684652238409Clontech Universal Primer Mix Long (96% over 25bp)
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC23170.15068184020441225No Hit
AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG22350.1453491207841439Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCA21480.13969123554556645No Hit
ACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACGGG21150.13754514114472674No Hit
GGATGGCACTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCT21020.13669971001712325No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20680.1344885824526217No Hit
CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA20620.13409838347065087Clontech Universal Primer Mix Long (96% over 25bp)
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA20370.1324725543791056No Hit
GCTATAGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACC20340.1322774548881202No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG20090.13065162579657497Clontech SMART CDS Primer II A (96% over 26bp)
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA19840.12902579670502973Clontech SMART CDS Primer II A (96% over 26bp)
GCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATTGCCTCGA19780.12863559772305885No Hit
GGGATACCCGAGCCAGCATGCATATCTTCAATGACGACGGTTGGTTTACC19540.12707480179517547No Hit
GCCTTTGGTGATGCGATGAACGATCGCGAAATGTTAGTCAGCGTCGGTAG17930.11660446244562414No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT17230.1120521409892975Clontech SMART CDS Primer II A (96% over 26bp)
GTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGA16860.10964591393381054No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16580.10782498535127989No Hit
CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC15970.1038579623679095Clontech SMARTer II A Oligonucleotide (95% over 24bp)
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA15940.10366286287692408No Hit
GATATGGATGGCACTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAAC15780.10262233225833514No Hit
GCATTGACGGTGCTGACCCAACATTTAGGTTTATCGTTGCGCGATTGCAC15660.10184193429439344No Hit
CTATCGCTGGATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTC15650.10177690113073162No Hit
ACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGC15590.10138670214876076No Hit
CTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCG15550.10112656949411351No Hit
ATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGTTCCGTTGCCGGTAA15550.10112656949411351No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)