FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001320647

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001320647
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1833765
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT205661.1215177517293655Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT98650.5379642429646111Clontech Universal Primer Mix Long (96% over 25bp)
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT76220.4156475884314511Clontech Universal Primer Mix Long (96% over 25bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73210.39923327143881576No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC67360.36733169190163406Clontech Universal Primer Mix Long (96% over 25bp)
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT63110.344155330699408Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60850.3318309598012832No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC53800.2933854665128847Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GTACATGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAACTT51870.2828606718963444No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT50410.27489891016569734Clontech Universal Primer Mix Long (96% over 25bp)
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC50000.27266307296736497Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA42830.23356318830384482Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA39760.21682167562364862Clontech Universal Primer Mix Long (96% over 25bp)
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG39580.21584008856096612Clontech Universal Primer Mix Long (96% over 25bp)
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG37140.20253413060015868No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC35700.1946814340986986Clontech Universal Primer Mix Long (96% over 25bp)
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA34480.18802845511829488Clontech Universal Primer Mix Long (96% over 25bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32740.17853978017903058No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA31800.17341371440724412Clontech Universal Primer Mix Long (96% over 25bp)
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC29260.15956243030050196Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC24740.13491368850425217Clontech Universal Primer Mix Long (96% over 25bp)
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCG24260.13229612300376548No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTG23810.1298421553470592No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT23090.12591580709632916Clontech SMART CDS Primer II A (96% over 26bp)
CTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTT21930.11959002380348627No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA21820.11899016504295806No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA21410.11675432784462568Clontech Universal Primer Mix Long (96% over 25bp)
GAGTACATGGGGTGGCGGCGACGACCCATTCGAACGTCTGCCCTATCAAC21140.11528194725060191No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG20910.11402769711495203Clontech Universal Primer Mix Long (96% over 25bp)
GTATCAACGCAGAGTACATGGGGTGGCGGCGACGACCCATTCGAACGTCT20740.11310064266686298No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCA20480.1116827946874327No Hit
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTT20000.10906522918694599No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT19570.10672032675942665Clontech Universal Primer Mix Long (96% over 25bp)
GTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACG19440.10601140276971151No Hit
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC19250.1049752830924355Clontech Universal Primer Mix Long (96% over 25bp)
ATTCGAACGTCTGCCCTATCAACTTTCGATGGTAGTCGCCGTGCCTACCA18870.10290304373788355No Hit
ACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCAT18700.10197598928979448No Hit
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG18570.10126706530007934Clontech Universal Primer Mix Long (96% over 25bp)
CTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTA18370.10017641300820988No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)