FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001320651

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001320651
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3182363
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT46439714.59283557532563Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT2485197.809259974427807Clontech Universal Primer Mix Long (96% over 25bp)
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC1648865.1812442515200186Clontech Universal Primer Mix Long (96% over 25bp)
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT1583264.975108119344021Clontech Universal Primer Mix Long (96% over 25bp)
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT1288524.048940991332541Clontech SMARTer II A Oligonucleotide (95% over 24bp)
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC1086883.4153237704183965Clontech SMARTer II A Oligonucleotide (95% over 24bp)
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT1053823.3114387013675057Clontech Universal Primer Mix Long (96% over 25bp)
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC917972.8845546532560866Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA774122.432532052440278Clontech Universal Primer Mix Long (96% over 25bp)
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG765472.4053509923286565Clontech Universal Primer Mix Long (96% over 25bp)
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA762542.3961439974006735Clontech Universal Primer Mix Long (96% over 25bp)
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC709692.230072433597299Clontech Universal Primer Mix Long (96% over 25bp)
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC605241.9018572048506095Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC505101.58718537137341Clontech Universal Primer Mix Long (96% over 25bp)
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG428641.3469236539012048Clontech Universal Primer Mix Long (96% over 25bp)
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA406891.2785782137361452Clontech Universal Primer Mix Long (96% over 25bp)
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT385541.2114897012063048Clontech SMARTer II A Oligonucleotide (95% over 24bp)
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC382541.2020627439421587Clontech Universal Primer Mix Long (96% over 25bp)
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT374161.1757301099843103Clontech Universal Primer Mix Long (96% over 25bp)
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG343231.0785381805909635Clontech Universal Primer Mix Long (96% over 25bp)
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC341451.0729448526142367Clontech Universal Primer Mix Long (96% over 25bp)
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA316250.9937584115954088Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA283680.891413078897662Clontech Universal Primer Mix Long (96% over 25bp)
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT256450.805847730130095Clontech SMART CDS Primer II A (96% over 26bp)
ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG249300.7833801486505467Clontech Universal Primer Mix Long (96% over 25bp)
AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG215360.6767298388021731Clontech SMARTer II A Oligonucleotide (95% over 24bp)
AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA208200.6542308341317442Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCTTGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC186090.584754159094987Clontech SMART CDS Primer II A (96% over 26bp)
CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT161400.507170300811064Clontech Universal Primer Mix Long (96% over 25bp)
CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT160820.5053477557399958Clontech Universal Primer Mix Long (96% over 25bp)
ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA139570.43857347511896033Clontech Universal Primer Mix Long (96% over 25bp)
CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA131880.4144090413318657Clontech Universal Primer Mix Long (96% over 25bp)
TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA130940.4114552613890998Clontech Universal Primer Mix Long (96% over 25bp)
GAGCAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT122650.38540543614917594Clontech Universal Primer Mix Long (96% over 27bp)
GAGTAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA120320.3780838326740224Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG120320.3780838326740224Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GAGTACAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA116130.3649175156950983Clontech Universal Primer Mix Long (96% over 25bp)
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC112510.35354232059636187Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT110620.3476033375199498Clontech Universal Primer Mix Long (96% over 28bp)
CCCATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG105270.3307919303988891Clontech SMART CDS Primer II A (96% over 26bp)
GTACAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT96590.3035166007146262Clontech Universal Primer Mix Long (100% over 24bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC91000.28595103701243385Clontech SMART CDS Primer II A (96% over 26bp)
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG89180.2802320162721852Clontech SMART CDS Primer II A (96% over 26bp)
CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC86800.2727532968426292Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA84740.26628011952124886Clontech SMART CDS Primer II A (96% over 26bp)
GCTTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGC81620.2564760839665368Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT73220.23008060362692753Clontech SMART CDS Primer II A (96% over 26bp)
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC63380.1991601838005281Clontech SMART CDS Primer II A (96% over 26bp)
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG61320.19268700647914774Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTTGCTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT60770.19095873098072094Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT60300.18948184100933801Clontech Universal Primer Mix Long (96% over 25bp)
GCTTACTCTGCGTTGATACCACTGCTTGCTCTGCGTTGATACCACTGCTT55850.1754985210675212Clontech Universal Primer Mix Long (96% over 28bp)
CTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGC53130.1669514131480287Clontech Universal Primer Mix Long (96% over 25bp)
CATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT50820.15969265605463612Clontech SMART CDS Primer II A (96% over 26bp)
AGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTG50770.15953554010023369Clontech Universal Primer Mix Long (96% over 25bp)
TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG49210.1546335223228777Clontech Universal Primer Mix Long (96% over 25bp)
GCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAG46560.14630637673954855Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CTGCTTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT42660.13405133229615854Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GAGTACGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT42470.1334542916694293Clontech Universal Primer Mix Long (96% over 25bp)
CGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCA42070.1321973640342098Clontech Universal Primer Mix Long (96% over 25bp)
TCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAA39390.12377594887823921Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CTGCTTACTCTGCGTTGATACCACTGCTTGCTCTGCGTTGATACCACTGC38260.12022512830874416Clontech Universal Primer Mix Long (96% over 28bp)
GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT37960.11928243258232955Clontech Universal Primer Mix Long (96% over 25bp)
CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG37450.1176798498474247Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTGCTCTGCGT36120.1135005654603199Clontech Universal Primer Mix Long (96% over 28bp)
AGAGCAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG35030.11007543765434678Clontech Universal Primer Mix Long (96% over 28bp)
GCGTTGATACCACTGCTTGCTCTGCGTTGATACCACTGCTTACTCTGCGT34680.10897562597352974Clontech SMART CDS Primer II A (95% over 24bp)
AAAGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT34220.10753015919302732Clontech SMART CDS Primer II A (100% over 26bp)
CAGAGCAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA34030.10693311856629806Clontech Universal Primer Mix Long (100% over 25bp)
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTAT33260.10451353286850054Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT32640.10256529503391033Clontech Universal Primer Mix Long (96% over 28bp)
ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA32400.10181113845277864Clontech Universal Primer Mix Long (96% over 25bp)
ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG31950.10039709486315673Clontech SMART CDS Primer II A (96% over 26bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)