FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001320687

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001320687
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1448683
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT133230.9196628938145889Clontech SMARTer II A Oligonucleotide (95% over 24bp)
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCG93800.6474846463995229No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTG87910.6068270284113225No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA79160.546427341247188No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCA76820.5302747391941508No Hit
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTT75740.522819692092749No Hit
GTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACT72850.5028705382751092No Hit
ACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCAT68600.47353354736681524No Hit
CTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTA68080.4699440802439181No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT66420.45848539673620803Clontech Universal Primer Mix Long (96% over 25bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66380.4582092838806005No Hit
GTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACG64700.4466125439450867No Hit
GCATATTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTACCGGCAACG59630.41161523949683954No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC56300.3886288442675175No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56250.3882837031980081No Hit
GAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCAT55900.38586771571144274No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA55530.38331367179707365No Hit
GTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCG52160.3600511637121441No Hit
ACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACGGG51700.3568758658726581No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA51170.35321737053585917No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT50330.3474190005681022Clontech Universal Primer Mix Long (96% over 25bp)
CTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCA48870.33734088133842943No Hit
GCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATTGCCTCGA48310.3334753013599248No Hit
GCTATAGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACC47690.32919555209800905No Hit
GGATGGCACTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCT45290.3126287807615607No Hit
GGGATACCCGAGCCAGCATGCATATCTTCAATGACGACGGTTGGTTTACC43580.30082495618434124No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC41760.28826182125420124Clontech Universal Primer Mix Long (96% over 25bp)
GCCTTTGGTGATGCGATGAACGATCGCGAAATGTTAGTCAGCGTCGGTAG38740.2674153006558371No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT38420.26520639781097727Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCG37590.25947705605712224No Hit
GCATTGACGGTGCTGACCCAACATTTAGGTTTATCGTTGCGCGATTGCAC37440.25844163284859417No Hit
GTGCTGACCCAACATTTAGGTTTATCGTTGCGCGATTGCACGGCCTTTGG36810.2540928553727765No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC36300.25057241646378126Clontech SMARTer II A Oligonucleotide (95% over 24bp)
ACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCT34790.24014915616459914No Hit
GTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCA34600.23883762010046367No Hit
GGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTT34100.23538620940537028No Hit
GATATGGATGGCACTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAAC34050.23504106833586091No Hit
GGTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTC33650.2322799397797862No Hit
CTATCGCTGGATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTC33510.23131354478516003No Hit
AGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACCAACCG33270.22965686765151522No Hit
TCGATTATCTGATAACGAAAACCGCTATAGACAAATGCCTGCAACAACGC32600.22503197732009003No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT32480.22420363875326763Clontech Universal Primer Mix Long (96% over 25bp)
ACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGC32370.2234443284003471No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC31970.22068319984427237Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGA31520.2175769302186883No Hit
TATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGT31140.2149538580904173No Hit
AGAATATGCTGCATCTCCAGCGCATGACGCCCCGTGGCAAAAGTGAGGGT31010.21405649130969304No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30490.21046702418679586No Hit
GCACTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACT30120.20791298027242675No Hit
GCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACGGTTGGT29930.20660144420829124No Hit
ATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGTTCCGTTGCCGGTAA29250.20190752566296424No Hit
GTCATGCGCTGGAGATGCAGCATATTCTCGGGGCGCTATCGCTGGATGCG28900.19949153817639884No Hit
CTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCGGGGCG28730.1983180585400671No Hit
CCGGTAAATGCGGGAGCTCCGCGCGCAGTTGCGGCATCGCATTGCCCATA28370.19583304283959982No Hit
ACCCGAGCCAGCATGCATATCTTCAATGACGACGGTTGGTTTACCGGGAA28010.1933480271391326No Hit
TGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATA27620.19065592679695972No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA27100.18706645967406257Clontech Universal Primer Mix Long (96% over 25bp)
CAGCTATACGAAGCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCAC26550.18326990790945985No Hit
GCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGA25970.1792662715031515No Hit
CTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCACATTTGTGTT25920.17892113043364216No Hit
CCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTT25800.17809279186681973No Hit
ATCGCTGGATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTC25760.17781667901121226No Hit
TTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTACCGGCAACGGAACG25740.17767862258340852No Hit
GAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCT25660.17712639687219356No Hit
CATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATG25550.17636708651927302No Hit
GCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACCAACCGTCGTCATT25470.17581486080805808No Hit
GGAGATGCAGCATATTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTA25330.1748484658134319No Hit
GATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCT25150.17360595796319828No Hit
TGCATATCTTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCG25130.17346790153539457No Hit
CCTTTATTGCAGCCCACCGGCAGCACTTCGAGGCAATCCGTGGCGGAAAA25110.17332984510759084No Hit
CTTCTGTGGCGATCACGACGATCTTACACGCTTGCAGATCCAGCTATACG24740.17077580119322172No Hit
CTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCG24480.16898106763177315No Hit
GCATTTGATATGGATGGCACTTTATTGGTGCCCGACCATCATTTAGGTGA24460.1688430112039694No Hit
ATACGAAGCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATT24390.16835981370665634No Hit
GAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTT24240.1673243904981283No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG24150.16670313657301147Clontech Universal Primer Mix Long (96% over 25bp)
CAATAAAGGCGCTGCATTGACGGTGCTGACCCAACATTTAGGTTTATCGT24130.16656508014520774No Hit
CCTAAATGTTGGGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCAC23550.1625614437388994No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA23380.16138796410256764Clontech Universal Primer Mix Long (96% over 25bp)
CATATCTTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTT23280.16069768196354897No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC23200.160145456252334Clontech Universal Primer Mix Long (96% over 25bp)
ACCAAGATCTGCTTCTGTGGCGATCACGACGATCTTACACGCTTGCAGAT23100.15945517411331533No Hit
GAAATGGCTCGTCTGGCAGCATTTGATATGGATGGCACTTTATTGGTGCC22220.15338069128995094No Hit
ATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGC22190.15317360664824534No Hit
CTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACG22100.15255235272312853No Hit
CACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCGGGG22060.15227623986752103No Hit
GGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGG21820.15061956273387622No Hit
ACCTAAATGTTGGGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCA21810.15055053451997435No Hit
AAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTA21200.1463398134719604No Hit
GCAATAAAGGCGCTGCATTGACGGTGCTGACCCAACATTTAGGTTTATCG21200.1463398134719604No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC21120.14578758776074546Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CAACAACGCAGGGATCTCTTTCCCGGTAAACCAACCGTCGTCATTGAAGA21050.14530439026343236No Hit
GATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATAC21020.14509730562172676No Hit
TCTTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTG20980.1448211927661193No Hit
TCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATA20710.14295743099076885No Hit
GTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAAC20420.1409556127876147No Hit
TTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATC20360.14054144350420347No Hit
ATTATGGGCAATGCGATGCCGCAACTGCGCGCGGAGCTCCCGCATTTACC20330.14033435886249787No Hit
TCCAGCGATAGCGCCCCGAGAATATGCTGCATCTCCAGCGCATGACGCCC20120.13888476637055863No Hit
CATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATTGCCTCGAA20040.1383325406593437No Hit
CCGCTATAGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAA20040.1383325406593437No Hit
CTTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGC19800.13667586352569885No Hit
CATTTGTGTTTTTCCGCCACGGATTGCCTCGAAGTGCTGCCGGTGGGCTG19740.13626169424228765No Hit
CTATAGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACCA19250.1328793117610961No Hit
TTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGC18680.12894470356868964No Hit
TGCTGATACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATCACGATG18670.12887567535478775No Hit
GCATGCATATCTTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCT18580.12825442142967095No Hit
GTCTGGCAGCATTTGATATGGATGGCACTTTATTGGTGCCCGACCATCAT18420.12714997000724107No Hit
ATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGAC18410.1270809417933392No Hit
GATCTGCTTCTGTGGCGATCACGACGATCTTACACGCTTGCAGATCCAGC18320.12645968786822237No Hit
TCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCAT18060.1246649543067738No Hit
GCGCTGGAGATGCAGCATATTCTCGGGGCGCTATCGCTGGATGCGTATTT18010.12431981323726446No Hit
CATTTGATATGGATGGCACTTTATTGGTGCCCGACCATCATTTAGGTGAG17820.12300827717312897No Hit
GTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGG17620.12162771289509161No Hit
GCTATACGAAGCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGG17540.12107548718387666No Hit
ACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAA17470.12059228968656359No Hit
GGGTAATGTCGCGTTCACGCAGTCGCGCCAAAGTAGAGAGGGTTTTCTCA17440.12038520504485799No Hit
ACATTTAGGTTTATCGTTGCGCGATTGCACGGCCTTTGGTGATGCGATGA17430.12031617683095613No Hit
GCGTGAACGCGACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGG17430.12031617683095613No Hit
CGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGC17220.1188665843390169No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC17130.11824533041390006Clontech Universal Primer Mix Long (96% over 25bp)
CGCTGGATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTG17040.11762407648878326No Hit
AGCATGCATATCTTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCC16810.11603642756904028No Hit
CCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCAC16760.11569128649953095No Hit
TTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCC16690.11520808900221788No Hit
TCCCATTGCTGATACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATC16670.11507003257441414No Hit
GATCTCTTTCCCGGTAAACCAACCGTCGTCATTGAAGATATGCATGCTGG16660.11500100436051226No Hit
TGCGGGAGCTCCGCGCGCAGTTGCGGCATCGCATTGCCCATAATAAATCC16510.11396558115198424No Hit
GTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACT16410.11327529901296557No Hit
CAGTTGCGGCATCGCATTGCCCATAATAAATCCGCTACCGACGCTGACTA16390.11313724258516183No Hit
CGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTTCGAGGCAAT16050.11079028331249831No Hit
GAAGATATGCATGCTGGCTCGGGTATCCCATTGCTGATACAGCACCAGCT16020.11058319867079272No Hit
CTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCACA15880.10961680367616655No Hit
AGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATC15730.10858138046763853No Hit
ATCAAATACGCATCCAGCGATAGCGCCCCGAGAATATGCTGCATCTCCAG15710.10844332403983481No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATAT15560.10740790083130679No Hit
ATGTTAGTCAGCGTCGGTAGCGGATTTATTATGGGCAATGCGATGCCGCA15470.10678664690618996No Hit
CCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTG15460.1067176186922881No Hit
GAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGATGGCACTTTATTGG15420.10644150583668062No Hit
TCATTGAAGATATGCATGCTGGCTCGGGTATCCCATTGCTGATACAGCAC15060.10395649013621337No Hit
ATAGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACCAAC15040.10381843370840965No Hit
GGTAAATCATCACGATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGT15040.10381843370840965No Hit
CCGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTTCGAGGCAA15020.10368037728060589No Hit
CTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGA14980.10340426442499843No Hit
CATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGAT14950.10319717978329282No Hit
GGGATCTCTTTCCCGGTAAACCAACCGTCGTCATTGAAGATATGCATGCT14930.10305912335548909No Hit
CGATTATCTGATAACGAAAACCGCTATAGACAAATGCCTGCAACAACGCA14870.10264495407207787No Hit
ATGCATATCTTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGC14640.10105730515233492No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)