FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001320785

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001320785
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2111121
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT165920.7859331606288792Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT81970.3882771285966082Clontech Universal Primer Mix Long (96% over 25bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65500.3102617045635944No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT65120.30846171299513386Clontech Universal Primer Mix Long (96% over 25bp)
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTG64070.30348805208228236No Hit
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCG63760.3020196379080119No Hit
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTT56550.2678671663064315No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC55720.2639356057753203Clontech Universal Primer Mix Long (96% over 25bp)
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA55160.2612829866217995No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53240.2521882923811567No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCA51010.24162518396624355No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT49050.2323410169289207Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACT47960.22717788321938914No Hit
GTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACG47440.2247147368625484No Hit
ACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCAT47070.22296211349325784No Hit
CTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTA47000.22263053609906774No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC43770.20733060776715312Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCAT43630.2066674529787729No Hit
GCATATTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTACCGGCAACG43020.203777992829402No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT42670.2021201058584515Clontech Universal Primer Mix Long (96% over 25bp)
GTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCG40720.19288330702029868No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC38860.184072821974676Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA37710.17862547907012435No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC37230.17635180550996366No Hit
ACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACGGG36690.1737939227547829No Hit
CTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCA35540.16834657985023124No Hit
GCTATAGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACC34490.16337291893737974No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA33520.1587782036178883Clontech Universal Primer Mix Long (96% over 25bp)
GCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATTGCCTCGA33170.15712031664693782No Hit
GGATGGCACTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCT32510.15399401550171687No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG31480.14911509098720538Clontech Universal Primer Mix Long (96% over 25bp)
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA31280.14816772700380507No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30360.14380985268016375No Hit
ATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGTTCCGTTGCCGGTAA30110.1426256477009134No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC28370.13438358104533088Clontech Universal Primer Mix Long (96% over 25bp)
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA28350.13428884464699087Clontech Universal Primer Mix Long (96% over 25bp)
GGGATACCCGAGCCAGCATGCATATCTTCAATGACGACGGTTGGTTTACC28180.13348358526110063No Hit
GCCTTTGGTGATGCGATGAACGATCGCGAAATGTTAGTCAGCGTCGGTAG26600.12599940979223834No Hit
GGTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTC25720.12183100826527707No Hit
ACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGC25330.11998364849764652No Hit
CTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCG25210.11941523010760634No Hit
GATATGGATGGCACTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAAC25190.1193204937092663No Hit
GCATTGACGGTGCTGACCCAACATTTAGGTTTATCGTTGCGCGATTGCAC25070.11875207531922614No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC24950.11818365692918596Clontech SMARTer II A Oligonucleotide (95% over 24bp)
AGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACCAACCG24290.11505735578396502No Hit
ACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCT23960.11349420521135453No Hit
GTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGA23900.11320999601633445No Hit
GTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCA23660.1120731592362541No Hit
GTGCTGACCCAACATTTAGGTTTATCGTTGCGCGATTGCACGGCCTTTGG23530.11145737264704392No Hit
TATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGT23390.11079421785866372No Hit
TCGATTATCTGATAACGAAAACCGCTATAGACAAATGCCTGCAACAACGC23070.10927843548522326No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA23050.10918369908688322Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTATCGCTGGATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTC22490.10653107993336242No Hit
GCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACGGTTGGT22350.10586792514498222No Hit
AGAATATGCTGCATCTCCAGCGCATGACGCCCCGTGGCAAAAGTGAGGGT21850.1034995151864815No Hit
GCACTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACT21680.10269425580059124No Hit
GGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTT21600.10231531020723113No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC21120.10004163664707043Clontech Universal Primer Mix Long (96% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)