FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001320806

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001320806
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences155879
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT86955.57804450888189Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT51143.2807498123544545Clontech Universal Primer Mix Long (96% over 25bp)
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT40032.5680175007537898Clontech Universal Primer Mix Long (96% over 25bp)
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC37712.419184110752571Clontech Universal Primer Mix Long (96% over 25bp)
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT25081.6089402677717974Clontech SMARTer II A Oligonucleotide (95% over 24bp)
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT24281.5576184091506873Clontech Universal Primer Mix Long (96% over 25bp)
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC17961.1521757260439187Clontech SMARTer II A Oligonucleotide (95% over 24bp)
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC17281.1085521462159753Clontech SMARTer II A Oligonucleotide (95% over 24bp)
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC16791.0771175078105453Clontech Universal Primer Mix Long (96% over 25bp)
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG14880.9545865703526453Clontech Universal Primer Mix Long (96% over 25bp)
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA14520.9314917339731459Clontech Universal Primer Mix Long (96% over 25bp)
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA14310.9180197460851044No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC13970.8962079561711328Clontech Universal Primer Mix Long (96% over 25bp)
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA12750.81794212177394Clontech Universal Primer Mix Long (96% over 25bp)
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA12440.79805490155826No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC11880.762129600523483Clontech SMARTer II A Oligonucleotide (95% over 24bp)
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC10310.6614104529795547Clontech Universal Primer Mix Long (96% over 25bp)
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG10180.6530706509536243Clontech Universal Primer Mix Long (96% over 25bp)
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA9640.6184283963843751Clontech Universal Primer Mix Long (96% over 25bp)
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT9360.6004657458669866Clontech Universal Primer Mix Long (96% over 25bp)
ATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGTTCCGTTGCCGGTAA9280.5953335600048756No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCA9260.5940505135393478No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA8960.5748048165564316No Hit
GGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTT8460.5427286549182379No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG8130.52155838823703Clontech Universal Primer Mix Long (96% over 25bp)
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC7990.5125770629783357No Hit
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC7810.501029644788586Clontech Universal Primer Mix Long (96% over 25bp)
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT7620.48884070336607244Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CCTTTATTGCAGCCCACCGGCAGCACTTCGAGGCAATCCGTGGCGGAAAA6710.43046208918455986No Hit
GCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATTGCCTCGA6640.4259714265552127No Hit
GTGTAAGATCGTCGTGATCGCCACAGAAGCAGATCTTGGTGACGCTGCCG6420.4118579154344075No Hit
ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG6350.40736725280506036Clontech Universal Primer Mix Long (96% over 25bp)
GCTATAGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACC5600.35925301034776974No Hit
CGCCTAATGCTTCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGA5500.352837778020131No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA5390.3457810224597284Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGCTGATACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATCACGATG5200.33359208103721477No Hit
GTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACAGAAGC4790.3072896284938959No Hit
CCGGTAAATGCGGGAGCTCCGCGCGCAGTTGCGGCATCGCATTGCCCATA4630.2970252567696739No Hit
TCCCATTGCTGATACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATC4630.2970252567696739No Hit
GGTAAATCATCACGATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGT4490.2880439315109797No Hit
ATACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATCACGATGTAAAA4440.2848363153471603No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTG4370.28034565271781314No Hit
CTTCGAGGCAATCCGTGGCGGAAAAACACAAATGTGCACGCTCGCCTAAT4300.27585499008846603No Hit
CAGTTGCGGCATCGCATTGCCCATAATAAATCCGCTACCGACGCTGACTA4260.27328889715741056No Hit
CTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTA4230.2713643274591189No Hit
CCTAAATGTTGGGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCAC4170.26751518806253566No Hit
CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT4150.2662321415970079Clontech Universal Primer Mix Long (96% over 25bp)
CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT4100.26302452543318855Clontech Universal Primer Mix Long (96% over 25bp)
CGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTTCGAGGCAAT4050.2598169092693692No Hit
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCG3920.2514771072434388No Hit
GGGTATCCCATTGCTGATACAGCACCAGCTCCGCGACATCCGCAGGTAAA3900.25019406077791106No Hit
CCGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTTCGAGGCAA3840.24634492138132782No Hit
CAGCTATACGAAGCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCAC3800.24377882845027232No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA3790.24313730521750848Clontech Universal Primer Mix Long (96% over 25bp)
GCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACCAACCGTCGTCATT3680.23608054965710581No Hit
ACCTAAATGTTGGGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCA3650.2341559799588142No Hit
ACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCAT3610.23158988702775873No Hit
CTTCTGTGGCGATCACGACGATCTTACACGCTTGCAGATCCAGCTATACG3560.22838227086393934No Hit
AGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACCAACCG3480.22325008500182833No Hit
GCATTGACGGTGCTGACCCAACATTTAGGTTTATCGTTGCGCGATTGCAC3360.21555180620866182No Hit
GGCTCGGGTATCCCATTGCTGATACAGCACCAGCTCCGCGACATCCGCAG3250.20849505064825924No Hit
AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG3250.20849505064825924Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CCACAGAAGCAGATCTTGGTGACGCTGCCGAGTGGCATTTTTTTGACATC3210.20592895771720374No Hit
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTT3180.20400438801891208No Hit
CTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCACATTTGTGTT3050.19566458599298173No Hit
TGCGGGAGCTCCGCGCGCAGTTGCGGCATCGCATTGCCCATAATAAATCC3000.19245696982916236No Hit
GTGCTGACCCAACATTTAGGTTTATCGTTGCGCGATTGCACGGCCTTTGG2990.1918154465963985No Hit
AGAATATGCTGCATCTCCAGCGCATGACGCCCCGTGGCAAAAGTGAGGGT2970.19053240013087075No Hit
ATGCACGCGCGTTCCGTTGCCGGTAATCAAATACGCATCCAGCGATAGCG2900.1860417375015236No Hit
GGGTAATGTCGCGTTCACGCAGTCGCGCCAAAGTAGAGAGGGTTTTCTCA2900.1860417375015236No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT2840.18219259810494037Clontech SMART CDS Primer II A (96% over 26bp)
AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA2810.18026802840664874Clontech SMARTer II A Oligonucleotide (95% over 24bp)
ACCAAGATCTGCTTCTGTGGCGATCACGACGATCTTACACGCTTGCAGAT2740.17577736577730163No Hit
CAACAACGCAGGGATCTCTTTCCCGGTAAACCAACCGTCGTCATTGAAGA2740.17577736577730163No Hit
ATACGAAGCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATT2710.17385279607901No Hit
GCCTCGAAGTGCTGCCGGTGGGCTGCAATAAAGGCGCTGCATTGACGGTG2710.17385279607901No Hit
GTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACT2680.17192822638071836No Hit
AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG2660.17064517991519063Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GGATGGCACTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCT2650.17000365668242676No Hit
ATGCATGCTGGCTCGGGTATCCCATTGCTGATACAGCACCAGCTCCGCGA2640.16936213344966286No Hit
GTTGCGGCATCGCATTGCCCATAATAAATCCGCTACCGACGCTGACTAAC2590.1661545172858435No Hit
GAAGATATGCATGCTGGCTCGGGTATCCCATTGCTGATACAGCACCAGCT2570.16487147082031575No Hit
ACACAAATGTGCACGCTCGCCTAATGCTTCGTATAGCTGGATCTGCAAGC2560.16422994758755188No Hit
CGGGAGCTCCGCGCGCAGTTGCGGCATCGCATTGCCCATAATAAATCCGC2550.163588424354788No Hit
ATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACAGAAGCAGATCTTGGT2540.16294690112202412No Hit
CGTGTAAGATCGTCGTGATCGCCACAGAAGCAGATCTTGGTGACGCTGCC2520.16166385465649638No Hit
CATTTGTGTTTTTCCGCCACGGATTGCCTCGAAGTGCTGCCGGTGGGCTG2500.16038080819096862No Hit
CCTAATGCTTCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATC2480.1590977617254409No Hit
GCACCAGCTCCGCGACATCCGCAGGTAAATCATCACGATGTAAAAGTTCA2480.1590977617254409No Hit
GATCTGCTTCTGTGGCGATCACGACGATCTTACACGCTTGCAGATCCAGC2480.1590977617254409No Hit
TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA2470.158456238492677Clontech Universal Primer Mix Long (96% over 25bp)
GTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCG2470.158456238492677No Hit
ATCGCTGGATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTC2440.1565316687943854No Hit
GCTGATACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATCACGATGT2430.15589014556162153No Hit
CTATCGCTGGATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTC2370.15204100616503827No Hit
GCATATTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTACCGGCAACG2360.1513994829322744No Hit
TCCAGCGATAGCGCCCCGAGAATATGCTGCATCTCCAGCGCATGACGCCC2350.15075795969951053No Hit
TCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACAGAA2340.15011643646674666No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA2310.14819186676845503Clontech SMART CDS Primer II A (96% over 26bp)
GAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCAT2300.14755034353569116No Hit
GCATTGCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATC2280.1462672970701634No Hit
GCATCGCATTGCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCG2250.1443427273718718No Hit
CTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCA2250.1443427273718718No Hit
ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA2220.14241815767358015Clontech Universal Primer Mix Long (96% over 25bp)
CCTTCCAGAGAATGCACGCGCGTTCCGTTGCCGGTAATCAAATACGCATC2220.14241815767358015No Hit
TCGATTATCTGATAACGAAAACCGCTATAGACAAATGCCTGCAACAACGC2210.14177663444081628No Hit
CCGCTATAGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAA2130.13664444857870528No Hit
GAATGCACGCGCGTTCCGTTGCCGGTAATCAAATACGCATCCAGCGATAG2100.13471987888041365No Hit
GTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACG2100.13471987888041365No Hit
CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA2080.1334368324148859Clontech Universal Primer Mix Long (96% over 25bp)
GCTATACGAAGCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGG2070.13279530918212204No Hit
CAGCGATAGCGCCCCGAGAATATGCTGCATCTCCAGCGCATGACGCCCCG2050.13151226271659427No Hit
GGTATCCCATTGCTGATACAGCACCAGCTCCGCGACATCCGCAGGTAAAT2040.1308707394838304No Hit
GCTCCGCGACATCCGCAGGTAAATCATCACGATGTAAAAGTTCACCTTCC2020.12958769301830267No Hit
GCCTAATGCTTCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGAT2010.12894616978553877No Hit
GCTTCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACA1960.1257385536217194No Hit
CATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATTGCCTCGAA1960.1257385536217194No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG1950.12509703038895553Clontech SMART CDS Primer II A (96% over 26bp)
ACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACGGG1950.12509703038895553No Hit
ACGCAGGGATCTCTTTCCCGGTAAACCAACCGTCGTCATTGAAGATATGC1940.12445550715619166No Hit
GATATGGATGGCACTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAAC1930.12381398392342778No Hit
ATCAAATACGCATCCAGCGATAGCGCCCCGAGAATATGCTGCATCTCCAG1920.12317246069066391No Hit
CAATAAAGGCGCTGCATTGACGGTGCTGACCCAACATTTAGGTTTATCGT1900.12188941422513616No Hit
CTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCG1900.12188941422513616No Hit
CTTCCAGAGAATGCACGCGCGTTCCGTTGCCGGTAATCAAATACGCATCC1890.12124789099237229No Hit
CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT1890.12124789099237229Clontech Universal Primer Mix Long (96% over 25bp)
GCCTTTGGTGATGCGATGAACGATCGCGAAATGTTAGTCAGCGTCGGTAG1850.11868179806131679No Hit
TATGCATGCTGGCTCGGGTATCCCATTGCTGATACAGCACCAGCTCCGCG1850.11868179806131679No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT1840.11804027482855291Clontech SMART CDS Primer II A (96% over 26bp)
GTGCACGCTCGCCTAATGCTTCGTATAGCTGGATCTGCAAGCGTGTAAGA1840.11804027482855291No Hit
GCGCATGACGCCCCGTGGCAAAAGTGAGGGTAATGTCGCGTTCACGCAGT1810.11611570513026129No Hit
GATCTTGGTGACGCTGCCGAGTGGCATTTTTTTGACATCGATTATCTGAT1800.11547418189749742No Hit
GGTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTC1790.11483265866473354No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC1790.11483265866473354Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCACTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACT1750.11226656573367803No Hit
GCAATAAAGGCGCTGCATTGACGGTGCTGACCCAACATTTAGGTTTATCG1730.1109835192681503No Hit
CGATAAACCTAAATGTTGGGTCAGCACCGTCAATGCAGCGCCTTTATTGC1730.1109835192681503No Hit
GTTCATCGCATCACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAAT1710.10970047280262255No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT1710.10970047280262255Clontech Universal Primer Mix Long (96% over 26bp)
TTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTACCGGCAACGGAACG1690.1084174263370948No Hit
TACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATCACGATGTAAAAG1630.10456828694051154No Hit
CCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCAC1610.10328524047498379No Hit
GATATGCATGCTGGCTCGGGTATCCCATTGCTGATACAGCACCAGCTCCG1610.10328524047498379No Hit
CTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCACA1590.10200219400945604No Hit
TCATTGAAGATATGCATGCTGGCTCGGGTATCCCATTGCTGATACAGCAC1590.10200219400945604No Hit
CTATAGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACCA1590.10200219400945604No Hit
CCCCGAGAATATGCTGCATCTCCAGCGCATGACGCCCCGTGGCAAAAGTG1580.10136067077669217No Hit
CACCAGCTCCGCGACATCCGCAGGTAAATCATCACGATGTAAAAGTTCAC1580.10136067077669217No Hit
AATGTGCACGCTCGCCTAATGCTTCGTATAGCTGGATCTGCAAGCGTGTA1580.10136067077669217No Hit
GAAGCAGATCTTGGTGACGCTGCCGAGTGGCATTTTTTTGACATCGATTA1570.1007191475439283No Hit
ATGCGGGAGCTCCGCGCGCAGTTGCGGCATCGCATTGCCCATAATAAATC1570.1007191475439283No Hit
ACGATAAACCTAAATGTTGGGTCAGCACCGTCAATGCAGCGCCTTTATTG1570.1007191475439283No Hit
CCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTT1560.10007762431116442No Hit
ATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCATCACCA1560.10007762431116442No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)