FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001320844

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001320844
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2293916
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA92070.4013660482772691No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88540.3859775161775758No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA82330.3589059058832146No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA78200.34090175926232696No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68970.3006648892112876No Hit
ATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGTTCCGTTGCCGGTAA66390.289417746770152No Hit
GTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACAGAAGC65180.2841429241524101No Hit
GTGTAAGATCGTCGTGATCGCCACAGAAGCAGATCTTGGTGACGCTGCCG54560.23784654712727055No Hit
CCTTTATTGCAGCCCACCGGCAGCACTTCGAGGCAATCCGTGGCGGAAAA53330.23248453735882219No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCA52190.22751486976855298No Hit
CGCCTAATGCTTCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGA51210.2232426993839356No Hit
GGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTT49990.21792428319084045No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC49240.21465476503934755No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT44970.1960403083635146Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCTATAGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACC44340.19329391311626057No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTG44280.19303235166414112No Hit
TGCTGATACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATCACGATG40140.17498461146790031No Hit
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCG39730.17319727487841752No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36180.15772155562801776No Hit
GGTAAATCATCACGATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGT35500.15475719250399753No Hit
CTTCGAGGCAATCCGTGGCGGAAAAACACAAATGTGCACGCTCGCCTAAT34220.14917721485878296No Hit
GCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATTGCCTCGA34000.14821815620101172No Hit
CCGGTAAATGCGGGAGCTCCGCGCGCAGTTGCGGCATCGCATTGCCCATA33630.14660519391294188No Hit
GGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTTAATTGTC32890.14337926933680223No Hit
ACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCAT32250.14058928051419495No Hit
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTT32130.14006615760995608No Hit
TCCCATTGCTGATACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATC32090.1398917833085431No Hit
AGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACCAACCG31750.138409601746533No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACA30830.13439899281403506No Hit
CCTAAATGTTGGGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCAC30190.13160900399142778No Hit
TCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACAGAA29930.13047557103224355No Hit
ATACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATCACGATGTAAAA29840.1300832288540644No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATAT29080.12677011712721825No Hit
ACCTAAATGTTGGGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCA28790.12550590344197432No Hit
CCACAGAAGCAGATCTTGGTGACGCTGCCGAGTGGCATTTTTTTGACATC28710.12515715483914844No Hit
ATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTGACGC28470.1241109090306707No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC28300.12336981824966563No Hit
CCTAATGCTTCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATC28090.12245435316724762No Hit
CGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTTCGAGGCAAT27360.1192720221664612No Hit
TCGATTATCTGATAACGAAAACCGCTATAGACAAATGCCTGCAACAACGC27110.11818218278263024No Hit
ATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACAGAAGCAGATCTTGGT26960.11752827915233165No Hit
GTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCG26790.11678718837132658No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT26630.11608969116567477Clontech Universal Primer Mix Long (96% over 25bp)
GAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCAT26240.11438954172689846No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCT26130.11391001239801284No Hit
GCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACCAACCGTCGTCATT25710.1120790822331768No Hit
GCCTTTGGTGATGCGATGAACGATCGCGAAATGTTAGTCAGCGTCGGTAG25130.10955065486268896No Hit
CTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCA25030.10911471910915657No Hit
CCGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTTCGAGGCAA24580.10715300821826082No Hit
CTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTA24370.10623754313584281No Hit
GTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGA24340.10610676240978309No Hit
AGAATATGCTGCATCTCCAGCGCATGACGCCCCGTGGCAAAAGTGAGGGT23830.10388349006676793No Hit
TACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTG23270.10144224984698655No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)