FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001320973

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001320973
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1774896
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT60390.3402452876112178No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC52260.2944397868945561No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47600.26818472744318544No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC43520.2451974650909124No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA40380.2275062876923493No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39410.222041178750755No Hit
GTCCTAATCTATTCTGCATCCACTTTGCAAAGTGGGGCCCCATCAAGGTT38390.21629436316268671No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT37440.21094193687968193No Hit
AATCTATTCTGCATCCACTTTGCAAAGTGGGGCCCCATCAAGGTTCACTG35990.2027724441319379No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG35040.19742001784893312No Hit
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCG33400.1881800398445881No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG31880.17961615779178047No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTG31710.1786583551937691No Hit
GATTAGGACCTTAGGGGCCTCCCCTGGTTTCTGCTGATACCAATTTAAAT31090.17516519277749232No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG29580.1666576520539795No Hit
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTT28670.1615305910881539No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC28500.16057278849014253No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT27410.15443158359701076No Hit
ACTTACTACTGTCAACAGAGTTACGTTCTCCCTCAAGCGTTCGGCCCAGG25950.14620574951997187No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG25800.14536062958055007No Hit
GTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACG25710.144853557616897No Hit
GTCGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTGCCGA25640.14445916831183347No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT25300.1425435631158107No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA25120.14152941918850456No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC24260.13668406486915288No Hit
ACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCAT24240.13657138221056334No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGGCGATGGTGAGAGCGA24240.13657138221056334No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG23800.13409236372159272No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTCGGAGACAGAGTCACCATCACTT23360.13161334523262208No Hit
GTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACT23140.1303738359881368No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCA23130.130317494658842No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA23070.12997944668307326No Hit
GTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCG23030.12975408136589411No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA22970.1294160333901254No Hit
GCATATTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTACCGGCAACG22960.12935969206083062No Hit
CTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTA22930.12919066807294624No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG22090.12445799641218416No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC22000.12395092444853106No Hit
GAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCAT21970.12378190046064672No Hit
GAGTTACGTTCTCCCTCAAGCGTTCGGCCCAGGGACCAGGATTGAAATCA21660.12203531925250831No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT21620.12180995393532917No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT21450.12085215133731779Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCAATCCTGGTCC20980.11820410886046281No Hit
CCCTCAAGCGTTCGGCCCAGGGACCAGGATTGAAATCAAACGAACTGTGG20570.11589411435937655No Hit
CCCCTAAGGTCCTAATCTATTCTGCATCCACTTTGCAAAGTGGGGCCCCA20340.11459826378559645No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC20280.11426021580982773No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC20250.11409119182194337No Hit
GAATAGATTAGGACCTTAGGGGCCTCCCCTGGTTTCTGCTGATACCAATT20240.11403485049264858No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC20000.11268265858957369No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19850.11183753865015189No Hit
AGTCAGAGCATTGCCGACAATTTAAATTGGTATCAGCAGAAACCAGGGGA19760.11133046668649882No Hit
ACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACGGG19700.1109924187107301No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19680.11087973605214052No Hit
TCCCTGGGCCGAACGCTTGAGGGAGAACGTAACTCTGTTGACAGTAGTAA19400.10930217883188649No Hit
CCTTAGGGGCCTCCCCTGGTTTCTGCTGATACCAATTTAAATTGTCGGCA19130.10778096294092725No Hit
GCCCAGGGACCAGGATTGAAATCAAACGAACTGTGGCTGCACCATCTGTC18990.10699218433080024No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC18980.10693584300150545No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT18920.10659779502573673No Hit
GTGCAGCCACAGTTCGTTTGATTTCAATCCTGGTCCCTGGGCCGAACGCT18530.10440048318324004No Hit
GGATGGCACTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCT18530.10440048318324004No Hit
CTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCA18380.10355536324381824No Hit
GTCTGCATCTGTCGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA18160.10231585399933293No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG17950.10113268608414239No Hit
AACCAGGGGAGGCCCCTAAGGTCCTAATCTATTCTGCATCCACTTTGCAA17840.10051293146189973No Hit
ATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTGAAAGATGAGCTGGAGGA17780.100174883486131No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)