FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001320974

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001320974
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1774896
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT55960.3152860787336272No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC46490.2619308398914641No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA43930.24750745959199863No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43470.24491575844443844No Hit
GTCCTAATCTATTCTGCATCCACTTTGCAAAGTGGGGCCCCATCAAGGTT39450.2222665440679341No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC38850.21888606431024693No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA38240.2154492432232649No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT35860.20204000685110565No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA35130.1979270898125862No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA33280.18750394389305064No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32660.18401078147677385No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG31210.17584128872902977No Hit
AATCTATTCTGCATCCACTTTGCAAAGTGGGGCCCCATCAAGGTTCACTG30790.1734749528986487No Hit
ATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGTTCCGTTGCCGGTAA29700.16733374800551695No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG28320.15955864456283636No Hit
GATTAGGACCTTAGGGGCCTCCCCTGGTTTCTGCTGATACCAATTTAAAT28170.15871352462341456No Hit
GTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACAGAAGC27520.1550513382192534No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT26430.14891013332612163No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT26370.1485720853503529No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC25130.14158576051779936No Hit
ACTTACTACTGTCAACAGAGTTACGTTCTCCCTCAAGCGTTCGGCCCAGG25070.14124771254203064No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG25060.14119137121273584No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG24230.13651504088126853No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC23710.13358529175793962No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC23610.13302187846499175No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA23310.13133163858614813No Hit
GTCGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTGCCGA23290.13121895592755858No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTCGGAGACAGAGTCACCATCACTT23090.13009212934166284No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC23050.1298667640244837No Hit
GTGTAAGATCGTCGTGATCGCCACAGAAGCAGATCTTGGTGACGCTGCCG22990.12952871604871496No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG22940.12924700940224104No Hit
GAGTTACGTTCTCCCTCAAGCGTTCGGCCCAGGGACCAGGATTGAAATCA22940.12924700940224104No Hit
CGCCTAATGCTTCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGA22600.12733140420621827No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA22120.12462702040006851No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGGCGATGGTGAGAGCGA22090.12445799641218416No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCA21750.12254239121616141No Hit
CCTTTATTGCAGCCCACCGGCAGCACTTCGAGGCAATCCGTGGCGGAAAA21740.12248604988686661No Hit
GGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTT21270.11983800741001162No Hit
CCCTCAAGCGTTCGGCCCAGGGACCAGGATTGAAATCAAACGAACTGTGG21180.11933093544635855No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT20750.11690825828668272No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT20050.11296436523604762No Hit
CCCCTAAGGTCCTAATCTATTCTGCATCCACTTTGCAAAGTGGGGCCCCA19970.11251363460168934No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG19600.11042900541778224No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT19370.10913315484400213No Hit
GAATAGATTAGGACCTTAGGGGCCTCCCCTGGTTTCTGCTGATACCAATT19110.10766828028233766No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC18990.10699218433080024No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT18910.10654145369644193Clontech SMARTer II A Oligonucleotide (95% over 24bp)
TGCTGATACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATCACGATG18720.10547096843984097No Hit
GTCTGCATCTGTCGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA18630.1049638964761879No Hit
CCTTAGGGGCCTCCCCTGGTTTCTGCTGATACCAATTTAAATTGTCGGCA18540.10445682451253482No Hit
GTAATATATGGCCGTGTCGTCAGATGCCAGGCTCCTGAGCTCCATGTAGG18530.10440048318324004No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCAATCCTGGTCC18340.10332999792663908No Hit
GCTATAGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACC17960.10118902741343717No Hit
AGTCAGAGCATTGCCGACAATTTAAATTGGTATCAGCAGAAACCAGGGGA17930.10102000342555283No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)