FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001320975

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001320975
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1811434
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT59760.32990437410361073No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC54730.30213631851891926No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50440.27845342419320823No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC43690.2411901289254811No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41560.2294314890854428No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA41320.22810657192036807No Hit
GTCCTAATCTATTCTGCATCCACTTTGCAAAGTGGGGCCCCATCAAGGTT38420.21209715617571492No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT37100.20481011176780384No Hit
AATCTATTCTGCATCCACTTTGCAAAGTGGGGCCCCATCAAGGTTCACTG35850.19790950153303954No Hit
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCG35730.1972470429505022No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG35520.1960877404310618No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTG33430.1845499201185359No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG31340.17301209980601004No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG30210.16677394815378313No Hit
GATTAGGACCTTAGGGGCCTCCCCTGGTTTCTGCTGATACCAATTTAAAT29420.16241276248541212No Hit
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTT28690.1583828061083098No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC28060.1549048985499886No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT27040.14927400059842091No Hit
ACTTACTACTGTCAACAGAGTTACGTTCTCCCTCAAGCGTTCGGCCCAGG26700.14739703461456505No Hit
GTCGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTGCCGA26150.14436076611126875No Hit
GTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACG25940.14320146359182834No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT25840.1426494147730472No Hit
ACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCAT24980.13790179493152938No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG24680.13624564847518594No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA24370.13453429713696444No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA24370.13453429713696444No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCA24370.13453429713696444No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGGCGATGGTGAGAGCGA24290.13409265808193951No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG24230.13376142879067082No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC24010.1325469213893523No Hit
GTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCG23690.13078036516925265No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA23540.12995229194108093No Hit
GTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACT23310.12868257965788432No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTCGGAGACAGAGTCACCATCACTT23270.12846176013037183No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT22920.12652958926463787Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTA22900.1264191795008816No Hit
GAGTTACGTTCTCCCTCAAGCGTTCGGCCCAGGGACCAGGATTGAAATCA22870.12625356485524727No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC22600.1247630330445382No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG22260.12288606706068231No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT22010.12150594501372945No Hit
GCATATTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTACCGGCAACG21950.12117471572246077No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21910.12095389619494831No Hit
GAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCAT21670.11962897902987356No Hit
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCAATCCTGGTCC21620.11935295462048298No Hit
GTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGACTTCGC21370.11797283257353015No Hit
CCCCTAAGGTCCTAATCTATTCTGCATCCACTTTGCAAAGTGGGGCCCCA21280.11747598863662712No Hit
CCTTAGGGGCCTCCCCTGGTTTCTGCTGATACCAATTTAAATTGTCGGCA20970.11576463729840558No Hit
CCCTCAAGCGTTCGGCCCAGGGACCAGGATTGAAATCAAACGAACTGTGG20680.11416369572394026No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20540.11339082737764666No Hit
GGATGGCACTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCT20200.11151386139379078No Hit
GAATAGATTAGGACCTTAGGGGCCTCCCCTGGTTTCTGCTGATACCAATT20010.1104649686381066No Hit
AGTCAGAGCATTGCCGACAATTTAAATTGGTATCAGCAGAAACCAGGGGA20000.11040976375622849No Hit
ACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACGGG19350.10682144643415106No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC19260.10632460249724804No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT19240.10621419273349181No Hit
CCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCCCTGATGGGTGAC18980.10477886580466084No Hit
CTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCA18980.10477886580466084No Hit
TCCCTGGGCCGAACGCTTGAGGGAGAACGTAACTCTGTTGACAGTAGTAA18960.10466845604090462No Hit
GTGCAGCCACAGTTCGTTTGATTTCAATCCTGGTCCCTGGGCCGAACGCT18890.10428202186775781No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC18790.10372997304897667No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18290.10096972895507095No Hit
GCCCAGGGACCAGGATTGAAATCAAACGAACTGTGGCTGCACCATCTGTC18180.10036247525441169No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)