FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001320976

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001320976
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1811434
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT55810.3080984457617556No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC46610.2573099544338905No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45520.25129262230917604No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA43910.24240463632679965No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC40150.22164760074062873No Hit
GTCCTAATCTATTCTGCATCCACTTTGCAAAGTGGGGCCCCATCAAGGTT38970.21513342467901123No Hit
TCTCTACACAGTGAAACTTTTTTTAAAAAGAATAGTAAAGAAAATGCGGA38860.21452617097835197No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT36980.20414765318526648No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA35580.1964189697223305No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33740.18626127145675747No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA33140.1829489785440706No Hit
AATCTATTCTGCATCCACTTTGCAAAGTGGGGCCCCATCAAGGTTCACTG32060.17698685130123426No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG31600.174447426734841No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG30850.17030706059398246No Hit
ATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGTTCCGTTGCCGGTAA29510.16290960642231514No Hit
GATTAGGACCTTAGGGGCCTCCCCTGGTTTCTGCTGATACCAATTTAAAT29450.16257837713104645No Hit
GTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACAGAAGC27900.15402162043993875No Hit
GTGCTAGAGAATACGTACCATGAAATACATATATTTCATAAGGTTCAGTT26920.14861154201588356No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT26150.14436076611126875No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC25490.14071724390731322No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA25480.1406620390254351No Hit
ACTTACTACTGTCAACAGAGTTACGTTCTCCCTCAAGCGTTCGGCCCAGG25010.13806740957716374No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG24830.1370737217033577No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC24590.13574880453828292No Hit
GTGTAAGATCGTCGTGATCGCCACAGAAGCAGATCTTGGTGACGCTGCCG24010.1325469213893523No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC23880.1318292579249368No Hit
CGCCTAATGCTTCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGA23660.13061475052361832No Hit
GAGTTACGTTCTCCCTCAAGCGTTCGGCCCAGGGACCAGGATTGAAATCA23490.12967626753169037No Hit
GTAGTAAGTTGCAAAATCTTCAGGTTGCAGACTGGCGATGGTGAGAGCGA23250.1283513503666156No Hit
GTCGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGAGCATTGCCGA23200.12807532595722507No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG22740.1255359013908318No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG22640.12498385257205065No Hit
GTCTCCATCCTCCCTGTCTGCATCTGTCGGAGACAGAGTCACCATCACTT22190.1224996328875355No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC21950.12117471572246077No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA21690.1197393887936298No Hit
CCTTTATTGCAGCCCACCGGCAGCACTTCGAGGCAATCCGTGGCGGAAAA21570.11907693021109243No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCA21500.11869049603794563No Hit
GGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTT21480.11858008627418941No Hit
CCCCTAAGGTCCTAATCTATTCTGCATCCACTTTGCAAAGTGGGGCCCCA21150.11675832517221163No Hit
GGGTAGCAGTAACTATCTGATTATCCAGCTCCACTTCTGTAGGATCACAT20930.1155438177708931No Hit
GAGCAGGACAGCAAGGACAGCACCTACAGCCTCAGCAGCACCCTGACGCT20920.115488612889015No Hit
CCCTCAAGCGTTCGGCCCAGGGACCAGGATTGAAATCAAACGAACTGTGG20590.11366685178703724No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT20130.11112742722064396No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG20040.11063058328374094No Hit
GAATAGATTAGGACCTTAGGGGCCTCCCCTGGTTTCTGCTGATACCAATT20030.11057537840186284No Hit
TGCTGATACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATCACGATG19380.10698706107978541No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT19200.10599337320597935Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GATGAAGACAGATGGTGCAGCCACAGTTCGTTTGATTTCAATCCTGGTCC19140.10566214391471067No Hit
CTGTAGCACTGTCTTCATCACAGATATTGCTCTGGGTAGCAGTAACTATC19080.10533091462344199No Hit
CCTTAGGGGCCTCCCCTGGTTTCTGCTGATACCAATTTAAATTGTCGGCA18980.10477886580466084No Hit
GTAATATATGGCCGTGTCGTCAGATGCCAGGCTCCTGAGCTCCATGTAGG18860.10411640722212347No Hit
GTACAGTGGAAGGTGGATAACGCCCTCCAATCGGGTAACTCCCAGGAGAG18830.10395079257648912No Hit
GCTATAGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACC18740.1034539486395861No Hit
GCCCAGGGACCAGGATTGAAATCAAACGAACTGTGGCTGCACCATCTGTC18360.10135616312821777No Hit
GTCTGCATCTGTCGGAGACAGAGTCACCATCACTTGCCGGGCAAGTCAGA18160.10025206549065548No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)