FastQCFastQC Report
Sat 3 Sep 2022
EGAF00001321047

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001321047
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences862240
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82280.9542586750788643No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64920.7529226201521618No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA61190.7096632028205604No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTG58100.6738263128595287No Hit
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTT55580.6446001113379105No Hit
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCG55140.6394971237706439No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCA49370.5725784004453517No Hit
ACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCAT47450.5503108183336426No Hit
CTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTA47140.5467155316385229No Hit
GTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACT46480.5390610502876229No Hit
GTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACG45920.5325663388383745No Hit
GAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCAT44870.5203887548710335No Hit
GCATATTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTACCGGCAACG42430.4920903692707367No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39150.45404991649656706No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC38810.4501066988309519No Hit
ATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGTTCCGTTGCCGGTAA37170.43108647244386716No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA36920.42818704768973836No Hit
GCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATTGCCTCGA35830.41554555576173685No Hit
GCCTTTGGTGATGCGATGAACGATCGCGAAATGTTAGTCAGCGTCGGTAG35550.41229820003711265No Hit
GTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCG34830.4039478567452217No Hit
GCTATAGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACC32930.381912228613843No Hit
GGATGGCACTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCT31020.35976062349229915No Hit
CTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCA30950.3589487845611431No Hit
ACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACGGG30540.3541937279643719No Hit
GCATTGACGGTGCTGACCCAACATTTAGGTTTATCGTTGCGCGATTGCAC30390.3524540731118946No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA30090.34897476340694006No Hit
GGGATACCCGAGCCAGCATGCATATCTTCAATGACGACGGTTGGTTTACC28840.3344776396362961No Hit
GTGCTGACCCAACATTTAGGTTTATCGTTGCGCGATTGCACGGCCTTTGG28730.3332018927444795No Hit
GGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTT27500.3189367229541659No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26370.3058313230655038No Hit
GTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGA23450.27196604193727963No Hit
ACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGC23080.26767489330116906No Hit
CTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCG22780.26419558359621453No Hit
AGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACCAACCG21950.25456949341250695No Hit
CTATCGCTGGATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTC21890.25387363147151604No Hit
ACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCT21650.25109018370755243No Hit
GCACTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACT21630.2508582297272221No Hit
GATATGGATGGCACTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAAC21560.25004639079606605No Hit
TGCTGATACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATCACGATG21540.24981443681573579No Hit
GTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCA21520.24958248283540543No Hit
GGTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTC21510.24946650584524033No Hit
CAGCTATACGAAGCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCAC21260.24656708109111153No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT20640.23937650770087215Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACGGTTGGT20440.2370569678975691No Hit
CCTTTATTGCAGCCCACCGGCAGCACTTCGAGGCAATCCGTGGCGGAAAA20230.23462145110410096No Hit
AGAATATGCTGCATCTCCAGCGCATGACGCCCCGTGGCAAAAGTGAGGGT20070.2327658192614585No Hit
TATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGT19430.22534329189088884No Hit
TGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATA19110.221632028205604No Hit
TCGATTATCTGATAACGAAAACCGCTATAGACAAATGCCTGCAACAACGC19010.22047225830395248No Hit
GGTAAATCATCACGATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGT18740.21734087956949338No Hit
GTCATGCGCTGGAGATGCAGCATATTCTCGGGGCGCTATCGCTGGATGCG18490.21444145481536464No Hit
ACCCGAGCCAGCATGCATATCTTCAATGACGACGGTTGGTTTACCGGGAA18410.21351363889404343No Hit
TCCCATTGCTGATACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATC18230.21142605307107068No Hit
CCGGTAAATGCGGGAGCTCCGCGCGCAGTTGCGGCATCGCATTGCCCATA18170.21073019113007982No Hit
CTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCGGGGCG18160.21061421413991463No Hit
GAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCT18030.20910651326776766No Hit
CCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTT18000.20875858229727223No Hit
GCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGA17710.20539524958248281No Hit
GCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACCAACCGTCGTCATT17390.201683985897198No Hit
ATACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATCACGATGTAAAA16970.19681295231026164No Hit
GCATTTGATATGGATGGCACTTTATTGGTGCCCGACCATCATTTAGGTGA16930.19634904434960104No Hit
CCTAAATGTTGGGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCAC16900.19600111337910558No Hit
CAATAAAGGCGCTGCATTGACGGTGCTGACCCAACATTTAGGTTTATCGT16820.19507329745778437No Hit
CTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCACATTTGTGTT16630.19286973464464652No Hit
CATTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATG16610.1926377806643162No Hit
GAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTT16440.19066617183150864No Hit
ATCGCTGGATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTC16280.1888105399888662No Hit
GGAGATGCAGCATATTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTA16180.1876507700872147No Hit
CTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACTCG16060.18625904620523287No Hit
CTTCTGTGGCGATCACGACGATCTTACACGCTTGCAGATCCAGCTATACG15800.18324364446093894No Hit
ACCTAAATGTTGGGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCA15780.18301169048060864No Hit
ATACGAAGCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATT15660.18161996659862684No Hit
TGCATATCTTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCG15620.18115605863796624No Hit
GAAATGGCTCGTCTGGCAGCATTTGATATGGATGGCACTTTATTGGTGCC15410.17872054184449807No Hit
GATAATCGATGTCAAAAAAATGCCACTCGGCAGCGTCACCAAGATCTGCT15320.1776767489330117No Hit
GCAATAAAGGCGCTGCATTGACGGTGCTGACCCAACATTTAGGTTTATCG15140.17558916311003897No Hit
TTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTACCGGCAACGGAACG15030.1743134162182223No Hit
GGGTAATGTCGCGTTCACGCAGTCGCGCCAAAGTAGAGAGGGTTTTCTCA14940.17326962330673595No Hit
ATTATGGGCAATGCGATGCCGCAACTGCGCGCGGAGCTCCCGCATTTACC14900.17280571534607533No Hit
ACCAAGATCTGCTTCTGTGGCGATCACGACGATCTTACACGCTTGCAGAT14840.17210985340508442No Hit
CATATCTTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTT14630.16967433661161624No Hit
CATTTGTGTTTTTCCGCCACGGATTGCCTCGAAGTGCTGCCGGTGGGCTG14620.1695583596214511No Hit
GTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAAC14480.167934681759139No Hit
CCGCTATAGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAA14470.16781870476897381No Hit
CGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTTCGAGGCAAT14390.16689088884765263No Hit
GGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGG14300.16584709593616626No Hit
CACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCGGGG13860.1607441083688996No Hit
CATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATTGCCTCGAA13840.1605121543885693No Hit
TCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATA13680.15865652254592688No Hit
GATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATAC13610.15784468361477083No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13530.15691686769344962Clontech SMART CDS Primer II A (100% over 21bp)
CAACAACGCAGGGATCTCTTTCCCGGTAAACCAACCGTCGTCATTGAAGA13520.15680089070328448No Hit
GAAGATATGCATGCTGGCTCGGGTATCCCATTGCTGATACAGCACCAGCT13230.15343755798849507No Hit
CTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACG13190.15297365002783447No Hit
GATCTGCTTCTGTGGCGATCACGACGATCTTACACGCTTGCAGATCCAGC13070.15158192614585264No Hit
GCATGCATATCTTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCT13050.15134997216552237No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12890.14949434032287995No Hit
TCCAGCGATAGCGCCCCGAGAATATGCTGCATCTCCAGCGCATGACGCCC12780.14821859343106328No Hit
AAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTA12710.14740675449990723No Hit
ACATTTAGGTTTATCGTTGCGCGATTGCACGGCCTTTGGTGATGCGATGA12710.14740675449990723No Hit
CTATAGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACCA12680.14705882352941177No Hit
GTCTGGCAGCATTTGATATGGATGGCACTTTATTGGTGCCCGACCATCAT12660.14682686954908147No Hit
ATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGC12480.14473928372610873No Hit
CATTTGATATGGATGGCACTTTATTGGTGCCCGACCATCATTTAGGTGAG12380.14357951382445724No Hit
CGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGC12350.1432315828539618No Hit
GCTATACGAAGCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGG12280.14241974392280574No Hit
AGCATGCATATCTTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCC12260.1421877899424754No Hit
ATGTTAGTCAGCGTCGGTAGCGGATTTATTATGGGCAATGCGATGCCGCA12140.1407960660604936No Hit
CTTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGC12130.14068008907032845No Hit
GCGCTGGAGATGCAGCATATTCTCGGGGCGCTATCGCTGGATGCGTATTT12070.13998422712933756No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11970.13882445722768602No Hit
GTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGG11960.1387084802375209No Hit
GCGTGAACGCGACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGG11860.13754871033586938No Hit
GGGTATCCCATTGCTGATACAGCACCAGCTCCGCGACATCCGCAGGTAAA11700.13569307849322695No Hit
CGCTGGATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTG11670.1353451475227315No Hit
TCTTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTG11500.1333735386899239No Hit
TTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATC11430.13256169975876786No Hit
GATCTCTTTCCCGGTAAACCAACCGTCGTCATTGAAGATATGCATGCTGG11410.13232974577843754No Hit
ATGCATGCTGGCTCGGGTATCCCATTGCTGATACAGCACCAGCTCCGCGA11380.1319818148079421No Hit
GGCTCGGGTATCCCATTGCTGATACAGCACCAGCTCCGCGACATCCGCAG11330.13140192985711635No Hit
TCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCAT11320.1312859528669512No Hit
GCCTCGAAGTGCTGCCGGTGGGCTGCAATAAAGGCGCTGCATTGACGGTG11240.13035813694562998No Hit
CCGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTTCGAGGCAA11120.12896641306364817No Hit
CAGTTGCGGCATCGCATTGCCCATAATAAATCCGCTACCGACGCTGACTA11070.12838652811282242No Hit
ATCAAATACGCATCCAGCGATAGCGCCCCGAGAATATGCTGCATCTCCAG10730.12444331044720727No Hit
ATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGAC10710.12421135646687696No Hit
TTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGC10660.12363147151605121No Hit
AGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATC10610.12305158656522547No Hit
CATTTAGGTTTATCGTTGCGCGATTGCACGGCCTTTGGTGATGCGATGAA10330.11980423084060121No Hit
GAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGATGGCACTTTATTGG10250.11887641491928001No Hit
GTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACT10230.1186444609389497No Hit
ATGCATATCTTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGC10200.11829652996845426No Hit
TCATTGAAGATATGCATGCTGGCTCGGGTATCCCATTGCTGATACAGCAC10070.11678882909630728No Hit
GGGATCTCTTTCCCGGTAAACCAACCGTCGTCATTGAAGATATGCATGCT10040.11644089812581183No Hit
ACCATCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAA10020.11620894414548155No Hit
CCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCAC9990.11586101317498608No Hit
GCTGATACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATCACGATGT9890.11470124327333457No Hit
TGCGGGAGCTCCGCGCGCAGTTGCGGCATCGCATTGCCCATAATAAATCC9880.11458526628316942No Hit
GCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAG9870.11446928929300428No Hit
CGATTATCTGATAACGAAAACCGCTATAGACAAATGCCTGCAACAACGCA9850.11423733531267395No Hit
TTTGTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCC9820.11388940434217852No Hit
CGACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGC9820.11388940434217852No Hit
GGTTTATCGTTGCGCGATTGCACGGCCTTTGGTGATGCGATGAACGATCG9770.11330951939135275No Hit
CATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGAT9770.11330951939135275No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9710.11261365745036185No Hit
CCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTG9660.1120337724995361No Hit
ATGCACGCGCGTTCCGTTGCCGGTAATCAAATACGCATCCAGCGATAGCG9590.11122193356838005No Hit
GCGCATGACGCCCCGTGGCAAAAGTGAGGGTAATGTCGCGTTCACGCAGT9570.11098997958804972No Hit
ATAGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACCAAC9530.11052607162738912No Hit
ATGCAGCATATTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTACCGG9530.11052607162738912No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT9510.11029411764705882Clontech Universal Primer Mix Long (96% over 25bp)
CTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCACA9430.10936630172573762No Hit
CTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGA9390.10890239376507702No Hit
CAGCGATAGCGCCCCGAGAATATGCTGCATCTCCAGCGCATGACGCCCCG9330.10820653182408611No Hit
ACGCAGGGATCTCTTTCCCGGTAAACCAACCGTCGTCATTGAAGATATGC9320.10809055483392097No Hit
GATCCAGCTATACGAAGCATTAGGCGAGCGTGCACATTTGTGTTTTTCCG9240.10716273891259974No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9190.10658285396177399No Hit
GATATGCATGCTGGCTCGGGTATCCCATTGCTGATACAGCACCAGCTCCG9130.10588699202078308No Hit
CGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCACATTTGTG8930.10356745221748005No Hit
CGGGAGCTCCGCGCGCAGTTGCGGCATCGCATTGCCCATAATAAATCCGC8880.10298756726665428No Hit
AGCCAGCATGCATATCTTCAATGACGACGGTTGGTTTACCGGGAAAGAGA8870.10287159027648914No Hit
CGATAAACCTAAATGTTGGGTCAGCACCGTCAATGCAGCGCCTTTATTGC8810.10217572833549823No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)