FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001321078

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001321078
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1808816
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA107080.5919894560861911No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA91950.5083435794464446No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA87580.48418412928678206No Hit
ATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGTTCCGTTGCCGGTAA83700.46273363349284835No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT76160.4210489071304102Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACAGAAGC69040.3816861416528823No Hit
GTGTAAGATCGTCGTGATCGCCACAGAAGCAGATCTTGGTGACGCTGCCG65020.3594616588973118No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCA64980.3592405197654156No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC63900.35326976320421755No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTG60560.33480464569088286No Hit
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCG59380.32828104129994423No Hit
GGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTT57380.3172240847051331No Hit
CCTTTATTGCAGCCCACCGGCAGCACTTCGAGGCAATCCGTGGCGGAAAA56080.3100370629185058No Hit
GCTATAGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACC54660.3021866237361899No Hit
CGCCTAATGCTTCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGA53880.2978744106642135No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47530.262768573475688No Hit
CCGGTAAATGCGGGAGCTCCGCGCGCAGTTGCGGCATCGCATTGCCCATA46120.2549734190763461No Hit
GGTAAATCATCACGATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGT46080.2547522799444498No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT44940.24844981468540747Clontech Universal Primer Mix Long (96% over 25bp)
GCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATTGCCTCGA43600.241041653766884No Hit
TGCTGATACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATCACGATG43490.24043352115416936No Hit
ACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCAT42710.236121308082193No Hit
CTTCGAGGCAATCCGTGGCGGAAAAACACAAATGTGCACGCTCGCCTAAT41760.23086925369965766No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40120.2218025492919125No Hit
AGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACCAACCG38200.21118787096089378No Hit
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTT37490.2072626513697358No Hit
TCCCATTGCTGATACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATC37220.20576996222943628No Hit
ATACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATCACGATGTAAAA37020.20466426656995515No Hit
CCACAGAAGCAGATCTTGGTGACGCTGCCGAGTGGCATTTTTTTGACATC36990.20449841222103296No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT34790.19233575996674068Clontech Universal Primer Mix Long (96% over 25bp)
CTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTA34700.19183819691997417No Hit
TCGATTATCTGATAACGAAAACCGCTATAGACAAATGCCTGCAACAACGC34200.1890739577712714No Hit
CCTAAATGTTGGGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCAC34180.18896338820532327No Hit
CTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCA34110.18857639472450485No Hit
GCCTTTGGTGATGCGATGAACGATCGCGAAATGTTAGTCAGCGTCGGTAG33960.18774712297989402No Hit
AGAATATGCTGCATCTCCAGCGCATGACGCCCCGTGGCAAAAGTGAGGGT32980.18232921424843654No Hit
CCTAATGCTTCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATC32940.1821080751165403No Hit
ACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACGGG32700.180781240325163No Hit
ACCTAAATGTTGGGTCAGCACCGTCAATGCAGCGCCTTTATTGCAGCCCA32690.18072595554218893No Hit
GGATGGCACTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCT32340.17879098813809696No Hit
TCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACAGAA32290.17851456422322667No Hit
ATTATGGGCAATGCGATGCCGCAACTGCGCGCGGAGCTCCCGCATTTACC30860.17060884025793668No Hit
GATCTTGGTGACGCTGCCGAGTGGCATTTTTTTGACATCGATTATCTGAT29570.16347710325428347No Hit
GAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCAT29520.1632006793394132No Hit
ATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACAGAAGCAGATCTTGGT29060.1606575793226066No Hit
CGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTTCGAGGCAAT29030.16049172497368444No Hit
GCATATTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTACCGGCAACG28980.16021530105881418No Hit
GCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACCAACCGTCGTCATT28710.15872261191851464No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC28470.15739577712713732Clontech Universal Primer Mix Long (96% over 25bp)
GTGCTGACCCAACATTTAGGTTTATCGTTGCGCGATTGCACGGCCTTTGG28070.15518438580817506No Hit
GCATTGACGGTGCTGACCCAACATTTAGGTTTATCGTTGCGCGATTGCAC27920.15435511406356422No Hit
GTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCG26960.14904777489805487No Hit
CCGTCAATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTTCGAGGCAA26630.147223377059911No Hit
CAGTTGCGGCATCGCATTGCCCATAATAAATCCGCTACCGACGCTGACTA26310.14545426400474123No Hit
TGCGGGAGCTCCGCGCGCAGTTGCGGCATCGCATTGCCCATAATAAATCC25970.14357458138362333No Hit
ACACAAATGTGCACGCTCGCCTAATGCTTCGTATAGCTGGATCTGCAAGC25670.14191603789440163No Hit
CAACAACGCAGGGATCTCTTTCCCGGTAAACCAACCGTCGTCATTGAAGA25260.13964936179246534No Hit
GGGTAATGTCGCGTTCACGCAGTCGCGCCAAAGTAGAGAGGGTTTTCTCA24360.13467373132480032No Hit
CAGCTATACGAAGCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCAC24010.13273876392070835No Hit
AATGTGCACGCTCGCCTAATGCTTCGTATAGCTGGATCTGCAAGCGTGTA23980.1325729095717862No Hit
CTATCGCTGGATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTC23940.13235177043988997No Hit
TCCAGCGATAGCGCCCCGAGAATATGCTGCATCTCCAGCGCATGACGCCC23860.13190949217609751No Hit
GAAGATATGCATGCTGGCTCGGGTATCCCATTGCTGATACAGCACCAGCT23640.1306932269506683No Hit
GGTATCAACGCAGAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTC23550.13019566390390178No Hit
GGCTCGGGTATCCCATTGCTGATACAGCACCAGCTCCGCGACATCCGCAG23250.1285371204146801No Hit
CGTGTAAGATCGTCGTGATCGCCACAGAAGCAGATCTTGGTGACGCTGCC22820.12615987474679569No Hit
CCGCTATAGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAA22810.12610458996382165No Hit
GCCTAATGCTTCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGAT22810.12610458996382165No Hit
ACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCT22800.1260493051808476No Hit
GATATGGATGGCACTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAAC22480.12428019212567779No Hit
GCTTCGTATAGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACA22460.12416962255972967No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT22390.12378262907891129Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CTATAGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACCA22300.12328506603214479No Hit
ATGCACGCGCGTTCCGTTGCCGGTAATCAAATACGCATCCAGCGATAGCG22270.12311921168322261No Hit
GCTGATACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATCACGATGT22140.12240050950455988No Hit
CTTCTGTGGCGATCACGACGATCTTACACGCTTGCAGATCCAGCTATACG22000.12162652254292311No Hit
GCACTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACT21950.12135009862805284No Hit
ACCAAGATCTGCTTCTGTGGCGATCACGACGATCTTACACGCTTGCAGAT21950.12135009862805284No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT21850.12079725079831227Clontech Universal Primer Mix Long (96% over 25bp)
CGGGAGCTCCGCGCGCAGTTGCGGCATCGCATTGCCCATAATAAATCCGC21630.11958098557288303No Hit
GGGTATCCCATTGCTGATACAGCACCAGCTCCGCGACATCCGCAGGTAAA21620.11952570078990897No Hit
CTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCACATTTGTGTT21580.11930456165801274No Hit
ATCAAATACGCATCCAGCGATAGCGCCCCGAGAATATGCTGCATCTCCAG21480.1187517138282722No Hit
ATGCATGCTGGCTCGGGTATCCCATTGCTGATACAGCACCAGCTCCGCGA21470.11869642904529813No Hit
GATCTCTTTCCCGGTAAACCAACCGTCGTCATTGAAGATATGCATGCTGG21300.11775658773473918No Hit
GAAGCAGATCTTGGTGACGCTGCCGAGTGGCATTTTTTTGACATCGATTA21270.11759073338581702No Hit
ATACGAAGCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATT21120.11676146164120618No Hit
CTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCG20570.1137207985776331No Hit
CATTTGTGTTTTTCCGCCACGGATTGCCTCGAAGTGCTGCCGGTGGGCTG20330.11239396378625577No Hit
GCACCAGCTCCGCGACATCCGCAGGTAAATCATCACGATGTAAAAGTTCA20300.11222810943733359No Hit
GTGCACGCTCGCCTAATGCTTCGTATAGCTGGATCTGCAAGCGTGTAAGA20220.11178583117354114No Hit
GTTGCGGCATCGCATTGCCCATAATAAATCCGCTACCGACGCTGACTAAC19710.10896630724186429No Hit
GTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACG19680.10880045289294211No Hit
AGCTGGATCTGCAAGCGTGTAAGATCGTCGTGATCGCCACAGAAGCAGAT19670.10874516810996807No Hit
CGATTATCTGATAACGAAAACCGCTATAGACAAATGCCTGCAACAACGCA19550.10808175071427939No Hit
GATCTGCTTCTGTGGCGATCACGACGATCTTACACGCTTGCAGATCCAGC19250.10642320722505771No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC19210.1062020680931615Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCATTGCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATC19120.10570450504639498No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19110.10564922026342094No Hit
GGTAAATGCGGGAGCTCCGCGCGCAGTTGCGGCATCGCATTGCCCATAAT18630.10299555068066625No Hit
GAATGCACGCGCGTTCCGTTGCCGGTAATCAAATACGCATCCAGCGATAG18460.1020557093701073No Hit
GCATTTGATATGGATGGCACTTTATTGGTGCCCGACCATCATTTAGGTGA18450.10200042458713324No Hit
CTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCGGGGCG18430.10188985502118512No Hit
ATCGCTGGATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTC18290.10111586805954835No Hit
GAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCT18240.10083944414467808No Hit
GCCTCGAAGTGCTGCCGGTGGGCTGCAATAAAGGCGCTGCATTGACGGTG18220.10072887457872996No Hit
CCTTCCAGAGAATGCACGCGCGTTCCGTTGCCGGTAATCAAATACGCATC18220.10072887457872996No Hit
GGTATCCCATTGCTGATACAGCACCAGCTCCGCGACATCCGCAGGTAAAT18130.10023131153196346No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)