FastQCFastQC Report
Thu 1 Sep 2022
EGAF00001321255

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001321255
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1523
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA60.3939592908732764No Hit
CTTAAAACTTTTTTGCCTGAATTTTTTTGTTTTGTAAACTTTATCTGTAC50.3282994090610637No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC40.2626395272488509No Hit
ATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTGACGC40.2626395272488509No Hit
GGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTTAATTGTC30.1969796454366382No Hit
GCATCTCCAGCGCATGACGCCCCGTGGCAAAAGTGAGGGTAATGTCGCGT30.1969796454366382No Hit
ATTATGGGCAATGCGATGCCGCAACTGCGCGCGGAGCTCCCGCATTTACC30.1969796454366382No Hit
GGTGAAGGGAGACTCGAAGTACTCTGAGGCTTGTAGGAGGGTAAAATAGA30.1969796454366382No Hit
ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG30.1969796454366382Clontech Universal Primer Mix Long (96% over 25bp)
GTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACG30.1969796454366382No Hit
GGTATCAACGCAGAGTACATGGGGGAATTCGTGGAGAAAGAAATGGCTCG30.1969796454366382No Hit
GGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGG30.1969796454366382No Hit
CTCTGGAAGGTGAACTTTTACATCGTGATGATTTACCTGCGGATGTCGCG30.1969796454366382No Hit
ACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCAT30.1969796454366382No Hit
TGCATATCTTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCG30.1969796454366382No Hit
ATGGACTTTACTCAAAATAAAATCTGGAGAACTTTTTAAAATGTAGTTTT30.1969796454366382No Hit
CTTCAATGACGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGC30.1969796454366382No Hit
GCTTAAGATTTTAAGAGAAAATATGAAGACTTAGAAGAGTAGCATGAGGA20.13131976362442546No Hit
GAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCT20.13131976362442546No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA20.13131976362442546Clontech Universal Primer Mix Long (96% over 25bp)
GAGTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCAT20.13131976362442546No Hit
ATCCTAGGCCTACCCGCCACAGTACTGATCATTCTATTTCCCCCTCTATT20.13131976362442546No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA20.13131976362442546Clontech Universal Primer Mix Long (96% over 25bp)
GGAGATTGGTTTTTATCCGGTGGGAACCGGACCGTATGAACTGGATACCT20.13131976362442546No Hit
GCATCGCATTGCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCG20.13131976362442546No Hit
GAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGATATGGATGG20.13131976362442546No Hit
CGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCACATTTGTG20.13131976362442546No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA20.13131976362442546Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCG20.13131976362442546No Hit
CCTGTGTTGGGTTGACAGTGAGGGTAATAATGACTTGTTGGTTGATTGTA20.13131976362442546No Hit
ATGCAGCGCCTTTATTGCAGCCCACCGGCAGCACTTCGAGGCAATCCGTG20.13131976362442546No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACA20.13131976362442546No Hit
GTGCTGACCCAACATTTAGGTTTATCGTTGCGCGATTGCACGGCCTTTGG20.13131976362442546No Hit
AGAATATGCTGCATCTCCAGCGCATGACGCCCCGTGGCAAAAGTGAGGGT20.13131976362442546No Hit
GTAGGAAGCAGAAGAAAAAAGACAAGCTAGGAAACAAAAAGCTAAGGGCA20.13131976362442546No Hit
TCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCAC20.13131976362442546No Hit
TTCATAGGCTGTGCAGATACAATGGCAGGATCAAGTGTCTGATTTTCAAA20.13131976362442546No Hit
CGCTGGATGCGTATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTG20.13131976362442546No Hit
CTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTA20.13131976362442546No Hit
GCTATGAAAATTGGTAAACAAATGCAATTCTTCGGAGCAAGAGCTAACTT20.13131976362442546No Hit
GCTATACGAAGCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGG20.13131976362442546No Hit
ACCCAGGGCCGTGAAGACTTGATCGCACGCACCTTCGAATCCCTGCGCGG20.13131976362442546No Hit
ATACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATCACGATGTAAAA20.13131976362442546No Hit
GGGATACCCGAGCCAGCATGCATATCTTCAATGACGACGGTTGGTTTACC20.13131976362442546No Hit
CAGAAATTATAACAAATTGTCTCTCAGACCACAGAGCAATCAAACTAGAA20.13131976362442546No Hit
AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG20.13131976362442546Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CTCCGCGACATCCGCAGGTAAATCATCACGATGTAAAAGTTCACCTTCCA20.13131976362442546No Hit
ACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTTGA20.13131976362442546No Hit
ATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTA20.13131976362442546No Hit
GGAGATGCAGCATATTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTA20.13131976362442546No Hit
GACCCAACATTTAGGTTTATCGTTGCGCGATTGCACGGCCTTTGGTGATG20.13131976362442546No Hit
CCTACAACTTTTAAACTAAACTTTAAGCTGTTTAAGTCACCTTCATTTTA20.13131976362442546No Hit
CTCTACTTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCA20.13131976362442546No Hit
GCGCATGACGCCCCGTGGCAAAAGTGAGGGTAATGTCGCGTTCACGCAGT20.13131976362442546No Hit
GTGAAAATACCAAATCTACGTTTGAATGGTGTACCTGAAAGTGACAGGGA20.13131976362442546No Hit
TCCTATAACTAACTTGAGAAAAGCTGGAACTTAAGTTTAACAGTTATAGT20.13131976362442546No Hit
CAGCTATACGAAGCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCAC20.13131976362442546No Hit
TTATTGCAGCCCACCGGCAGCACTTCGAGGCAATCCGTGGCGGAAAAACA20.13131976362442546No Hit
GGTTTAGACGTCCGGGAATTGCATCTGTTTTTAAGCCTAATGTGGGGACA20.13131976362442546No Hit
ACTTAGAAGAGTAGCATGAGGAAGGAAAAGATAAAAGGTTTCTAAAACAT20.13131976362442546No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.13131976362442546No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)