FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001321303

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001321303
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2544705
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACA150370.5909132885737247No Hit
ATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTGACGC142030.5581393521056468No Hit
GGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTTAATTGTC133950.5263871450718256No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC106150.41714069017823285No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT83290.32730709453551593No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATAT81540.32043006949725017No Hit
TACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTG77650.30514342526933375No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCT75740.29763764365614087No Hit
GTGTTGGGTTGACAGTGAGGGTAATAATGACTTGTTGGTTGATTGTAGAT73690.28958170003988676No Hit
GTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTATGCGGAGGAG69850.2744915422416351No Hit
GTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGA66880.2628202483195498No Hit
CTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCG66140.25991224916051175No Hit
ACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATA64840.25480360198922863No Hit
ACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTGT61310.24093166005489833No Hit
ATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTA61060.23994922790657464No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA60430.23747349889279898No Hit
ATTCTATACCAACACCTATTCTGATTTTTCGGTCACCCTGAAGTTTATAT60390.2373163097490672No Hit
GTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCT55740.21904307179024682No Hit
GGCTTATGCGGAGGAGAATGTTTTCATGTTACTTATACTAACATTAGTTC52990.20823631815868637No Hit
ACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAA52350.20572129185897778No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAG51970.20422799499352579No Hit
TGCCTGTGTTGGGTTGACAGTGAGGGTAATAATGACTTGTTGGTTGATTG51030.20053405011582875No Hit
CAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTA49290.19369632236349596No Hit
GTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTGAGCTTGAACGCTTT48520.19067043134665906No Hit
CTATTAACCTACTGGGAGAACTCTCTGTGCTAGTAACCACATTCTCCTGA47150.1852867031738453No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATAT45020.1769163812701276No Hit
GAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCC44080.17322243639243054No Hit
GTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACG43350.17035373451932542No Hit
TAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGA41960.16489141177464578No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT40310.1584073595957095No Hit
ATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCA40300.15836806230977657No Hit
ACTTATACTAACATTAGTTCTTCTATAGGGTGATAGATTGGTCCAATTGG39490.15518498214920787No Hit
GGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGC39270.15432044185868304No Hit
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCG38680.15200190198863917No Hit
CTTCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTAT38640.15184471284490736No Hit
CTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTA38350.15070509155285192No Hit
ATATTATGCTTGGTTATAATTTTTCATCTTTCCCTTGCGGTACTATATCT38330.150626496980986No Hit
ACCTAAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACC37970.14921179468739992No Hit
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTG37860.1487795245421375No Hit
CTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTT37780.1484651462546739No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCA37640.14791498425161267No Hit
CTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC37270.14646098467209362No Hit
ACTTTAAATTTGCCCACAGAACCCTCTAAATCCCCTTGTAAATTTAACTG35000.13754050076531465No Hit
ACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCAT34810.13679385233258864No Hit
AGATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCTAAATC33690.13239255630809857No Hit
CTTTTAGGCCTACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTT33160.1303098001536524No Hit
GGGTTGACAGTGAGGGTAATAATGACTTGTTGGTTGATTGTAGATATTGG33110.13011331372398766No Hit
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAACAGCCCAA32830.12901298971786512No Hit
CTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGC31520.12386504526064906No Hit
GTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCCTGCGTCAGATTA31330.12311839682792307No Hit
ATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTTAATTGTCAGTTC31310.12303980225605718No Hit
TACTTATACTAACATTAGTTCTTCTATAGGGTGATAGATTGGTCCAATTG30800.12103564067347689No Hit
AATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTTAATTGTCAGTT30680.12056407324228151No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCA30280.11899218180496363No Hit
CATTAGTTCTTCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGT30040.11804904694257291No Hit
GTTCAGTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTGAGCTTGAAC29840.11726310122391398No Hit
GTCTATAGCGGTTTTCGTTATCAGATAATCGATGTCAAAAAAATGCCACT29000.11396212920554642No Hit
CTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTA28870.11345126448841811No Hit
TATATGGTGTGCTCACACGATAAACCCTAGGAAGCCAATTGATATCATAG27920.10971802232478814No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC27610.10849980646086678No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27450.10787104988593964No Hit
GGTTGACAGTGAGGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGG27440.10783175260000667No Hit
GCTATAGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACC27240.10704580688134774No Hit
GTACATGGGGGGAATTCGTGGAGAAAGAAATGGCTCGTCTGGCAGCATTT27180.10681002316575004No Hit
GCATATTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTACCGGCAACG26990.10606337473302407No Hit
ATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGTTCCGTTGCCGGTAA26940.10586688830335933No Hit
TGCTGATACAGCACCAGCTCCGCGACATCCGCAGGTAAATCATCACGATG26840.10547391544402986No Hit
AGTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCCTGCGTCAGATT26800.10531672630029808No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26130.10268380814279063No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)