FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001321305

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001321305
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2869407
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACA125330.43678014307485835No Hit
GGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTTAATTGTC114320.39840984565800525No Hit
ATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTGACGC107490.3746070181051346No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC86390.3010726606577596No Hit
CTATTAACCTACTGGGAGAACTCTCTGTGCTAGTAACCACATTCTCCTGA84580.29476473710421697No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATAT78010.2718680201170486No Hit
GTGTTGGGTTGACAGTGAGGGTAATAATGACTTGTTGGTTGATTGTAGAT72170.2515153827951211No Hit
GTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGA65620.22868836662069897No Hit
CTCTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCT65060.22673674386380185No Hit
TCATAGAAGAGCTTATCACCTTTCATGATCACGCCCTCATAATCATTTTC62310.21715288211118186No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTGAT58710.20460673581684297No Hit
GTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTATGCGGAGGAG56980.1985776155142857No Hit
CTCCTAAACACATCCGTATTACTCGCATCAGGAGTATCAATCACCTGAGC56830.19805485941868822No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCT55250.19254849521172843No Hit
TACTAGAAGTGTGAAAACGTAGGCTTGGATTAAGGCGACAGCGATTTCTA55150.1921999911479968No Hit
TACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTG54490.189899864327368No Hit
CTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCG53330.18585721718808104No Hit
ACATATAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATA50910.17742341884577545No Hit
TCTGAGATGTTAGTATTAGTTAGTTTTGTTGTGAGTGTTAGGAAAAGGGC50040.17439143349131023No Hit
ATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTA49050.17094124326036703No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA48580.16930327416082835No Hit
GTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCT47770.16648039124460212No Hit
TGCCTGTGTTGGGTTGACAGTGAGGGTAATAATGACTTGTTGGTTGATTG46490.1620195392288372No Hit
ACTATGGGTGTTAAATTTTTTACTCTCTCTACAAGGTTTTTTCCTAGTGT46010.16034671972292533No Hit
GGCTTATGCGGAGGAGAATGTTTTCATGTTACTTATACTAACATTAGTTC41660.14518679295059922No Hit
GTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTGAGCTTGAACGCTTT40960.14274726450447775No Hit
GTACTGATCATTCTATTTCCCCCTCTATTGATCCCCACCTCCAAATATCT40920.1426078628789851No Hit
CAATTGGACCAATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTA40780.1421199571897608No Hit
GAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCC38940.13570748241709873No Hit
GGATAGTTCAGACGGTTTCTATTTCCTGAGCGTCTGAGATGTTAGTATTA38910.13560293119797923No Hit
CTTCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTAT37630.1311420791822143No Hit
TAACTGAACTCCTCACACCCAATTGGACCAATCTATCACCCTATAGAAGA36430.12696003041743467No Hit
CTCTACAAGGTTTTTTCCTAGTGTCCAAAGAGCTGTTCCTCTTTGGACTA35950.12528721091152284No Hit
GTGTATGAACATTAGGGTGTTTTCTCGTGTGAATGAGGGTTTTATGTTGT35420.12344013937374516No Hit
CTTGTAGAGAGAGTAAAAAATTTAACACCCATAGTAGGCCTAAAAGCAGC34670.12082635889575791No Hit
CCACAGTACTGATCATTCTATTTCCCCCTCTATTGATCCCCACCTCCAAA34640.12072180767663841No Hit
ATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCCGCA34020.11856108248150228No Hit
GGTGATAGCTGGTTGTCCAAGATAGAATCTTAGTTCAACTTTAAATTTGC33860.11800347597953166No Hit
GTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACG33480.11667916053735145No Hit
ACCTAAACCTACTCCAATGCTAAAACTAATCGTCCCAACAATTATATTAC33120.11542454590791756No Hit
ACTTATACTAACATTAGTTCTTCTATAGGGTGATAGATTGGTCCAATTGG33030.1151108922505591No Hit
GTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCCTGCGTCAGATTA32810.11434418331034948No Hit
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAACAGCCCAA31870.11106824511127211No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31650.11030153617106252No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAG31410.1094651264181066No Hit
GTATAGGGCTGTGACTAGTATGTTGAGTCCTGTAAGTAGGAGAGTGATAT31160.10859386625877751No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31090.10834991341416536No Hit
ACCTAAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGACC30720.10706044837835832No Hit
ACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAA30170.10514367602783434No Hit
CTAAATCCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTTT30140.10503912480871484No Hit
GGGTTGACAGTGAGGGTAATAATGACTTGTTGGTTGATTGTAGATATTGG29540.10294810042632502No Hit
CATCAGGAGTATCAATCACCTGAGCTCACCATAGTCTAATAGAAAACAAC28980.10099647766942788No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCA28830.1004737215738304No Hit
ATTACTATCCATCCTCATCCTAGCAATAATCCCCATCCTCCATATATCCA28770.10026461913559143No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)