FastQCFastQC Report
Fri 2 Sep 2022
EGAF00001321347

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001321347
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences474839
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34610.7288786304410547No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34420.7248772741918841No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21310.4487836929991007No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14430.3038924772396539No Hit
GGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTTAATTGTC9560.20133139864248725No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACA9550.2011208009451625No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA9160.19290749074949615No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8200.1726901118063175Clontech SMART CDS Primer II A (100% over 21bp)
ATTGTAGATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTGACGC8060.16974174404377063No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATAT7760.16342381312402732No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG7710.16237082463740343No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA7410.1560528937176601Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGACGACG6990.14720779043001944No Hit
GTGTTGGGTTGACAGTGAGGGTAATAATGACTTGTTGGTTGATTGTAGAT6730.14173225029957523No Hit
ATCTTACACGCTTGCAGATCCAGCTATACGAAGCATTAGGCGAGCGTGCA6730.14173225029957523No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTC6590.13878388253702834No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTCAAGCTCA6520.1373096986557549No Hit
TCATTTAGGTGAGAAAACCCTCTCTACTTTGGCGCGACTGCGTGAACGCG6510.13709910095843011No Hit
GTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGA6470.13625671016913102No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6460.13604611247180623No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT6440.13562491707715668Clontech Universal Primer Mix Long (96% over 26bp)
CTTTATTGGTGCCCGACCATCATTTAGGTGAGAAAACCCTCTCTACTTTG6340.13351894010390888No Hit
ATGTAAAAGTTCACCTTCCAGAGAATGCACGCGCGTTCCGTTGCCGGTAA5950.1253056299082426No Hit
GTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTATGCGGAGGAG5730.12067248056709748No Hit
GACTAACATTTCGCGATCGTTCATCGCATCACCAAAGGCCGTGCAATCGC5720.12046188286977269No Hit
ACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCAT5680.1196194920804736No Hit
TGCCTGTGTTGGGTTGACAGTGAGGGTAATAATGACTTGTTGGTTGATTG5680.1196194920804736No Hit
GCCTTTGGTGATGCGATGAACGATCGCGAAATGTTAGTCAGCGTCGGTAG5110.10761542333296127No Hit
GCTATAGACAAATGCCTGCAACAACGCAGGGATCTCTTTCCCGGTAAACC5090.10719422793831174No Hit
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAACAGCCCAA5080.10698363024098695No Hit
GCATATTCTCGGGGCGCTATCGCTGGATGCGTATTTGATTACCGGCAACG5020.10572004405703828No Hit
ATATTGGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTA5010.1055094463597135No Hit
CTTTTACATCGTGATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTA4940.10403526247844007No Hit
GCATTGACGGTGCTGACCCAACATTTAGGTTTATCGTTGCGCGATTGCAC4860.10235048089984183No Hit
GGCTTATGCGGAGGAGAATGTTTTCATGTTACTTATACTAACATTAGTTC4830.10171868780786751No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)