FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001321440

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001321440
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3459938
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCTGGGAGTTACCCGATTGGAGGGCGTTATCCACCTTCCACTGTACTT87790.2537328703577925No Hit
CTCTAACACTCTCCCCTGTTGAAGCTCTTTGTGACGGGCGAGCTCAGGCC77530.22407915979997328No Hit
GACTAACAGTATGCCGTACACGTTTGGCCAGGGGACCAAGCTGGACATCA69120.19977236586320332No Hit
GGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGC60760.1756100831864617No Hit
CTATAGTACATCGACTTTGAAAAGTGGGGTCCCATCAAGGTTCAGTGGCA56650.16373125761213062No Hit
CTGTTAGTCTGTTGACAGAAATATGTTGCAAAGTCTTCAGGTTGCAGGCT56520.16335552833605688No Hit
CTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGAT55010.15899128828320044No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53180.15370217616616252No Hit
CAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGGCGGGAAGATG51760.14959805638135712No Hit
GCATTAGCACTTATTTAAATTGGTATCAGCAAAAGTCAGGGAGAGCCCCT49170.14211237311188815No Hit
GCCCATAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCA48390.13985799745544575No Hit
GAGTTCACCCTCACCATTAGCAGCCTGCAACCTGAAGACTTTGCAACATA47780.13809496008309977No Hit
ACCAAAGGCCGTGCAATCGCGCAACGATAAACCTAAATGTTGGGTCAGCA44770.12939538222939254No Hit
GTCTACGCCTGCGAAGTCACCCATCAGGGCCTGAGCTCGCCCGTCACAAA43550.1258693074847006No Hit
GTCCTGCTCTGTGACACTCTCCTGGGAGTTACCCGATTGGAGGGCGTTAT42440.12266115751207102No Hit
ACAGTATGCCGTACACGTTTGGCCAGGGGACCAAGCTGGACATCAAAGGA42290.12222762373198595No Hit
GTAGGGGACAGAGTCACCATCACTTGCCGGGCGAGTCACAGCATTAGCAC42220.12202530796794624No Hit
ATCCCAGAGAGGCCAAAGTACAGTGGAAGGTGGATAACGCCCTCCAATCG41820.12086921788771939No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41320.11942410528743579No Hit
GTACTATAGATGATGAGATCAGGGGCTCTCCCTGACTTTTGCTGATACCA41160.11896166925534504No Hit
GGCTGGAACTGAGGAGCAGGTGGGGGCACTTCTCCCTCTAACACTCTCCC39780.11497315847856233No Hit
TAATAAATCCGCTACCGACGCTGACTAACATTTCGCGATCGTTCATCGCA39310.11361475263429577No Hit
CCTCCAACATTAGCATAATTAAAGCCAAGGAGGAGGAGGGGGGTGAGGTG38780.11208293327799516No Hit
CTCCCAGGAGAGTGTCACAGAGCAGGACAGCAAGGACAGCACCTACAGCC37290.10777649772915007No Hit
GTCTTCATCTTCCCGCCATCTGATGAGCAGTTGAAATCTGGAACTGCCTC36880.10659150539691753No Hit
CCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAA36870.10656260314491185No Hit
ATAGTACATCGACTTTGAAAAGTGGGGTCCCATCAAGGTTCAGTGGCAGT36370.10511749054462827No Hit
CAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGG35860.103643475692339No Hit
GTTGTGTGCCTGCTGAATAACTTCTATCCCAGAGAGGCCAAAGTACAGTG35840.10358567118832765No Hit
GCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCT34890.10083995724778884No Hit
GCATGGACATGAGGGTCCCCGCTCAGCTCCTGGGGCTCCTGCTACTCTGG34870.1007821527437775No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)