FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001321529

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001321529
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2440209
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT2114048.663356294481334Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT1397245.725902986178643Clontech Universal Primer Mix Long (96% over 25bp)
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC821683.3672525591045686Clontech Universal Primer Mix Long (96% over 25bp)
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT791693.2443532500699734Clontech Universal Primer Mix Long (96% over 25bp)
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT754373.0914155303910444Clontech SMARTer II A Oligonucleotide (95% over 24bp)
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC584992.3972946579575765Clontech SMARTer II A Oligonucleotide (95% over 24bp)
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC497512.038800774851662Clontech SMARTer II A Oligonucleotide (95% over 24bp)
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT479901.9666348251317818Clontech Universal Primer Mix Long (96% over 25bp)
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA421521.7273930224829104Clontech Universal Primer Mix Long (96% over 25bp)
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA394841.618058125349099Clontech Universal Primer Mix Long (96% over 25bp)
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG342751.4045928033213548Clontech Universal Primer Mix Long (96% over 25bp)
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC334111.369186000051635Clontech Universal Primer Mix Long (96% over 25bp)
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC309001.2662849780490113Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC234430.960696399365792Clontech Universal Primer Mix Long (96% over 25bp)
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA206050.8443948858478925Clontech Universal Primer Mix Long (96% over 25bp)
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT202520.8299289118268148Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG188180.7711634536222103Clontech Universal Primer Mix Long (96% over 25bp)
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC186330.7635821357924669Clontech Universal Primer Mix Long (96% over 25bp)
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT175670.7198973530545949Clontech Universal Primer Mix Long (96% over 25bp)
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC166640.6828923260261723Clontech Universal Primer Mix Long (96% over 25bp)
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG156250.640314005890479Clontech Universal Primer Mix Long (96% over 25bp)
CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA108180.44332268260628493Clontech Universal Primer Mix Long (96% over 25bp)
ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG106230.4353315638127718Clontech Universal Primer Mix Long (96% over 25bp)
AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA102050.4182018835271897Clontech SMARTer II A Oligonucleotide (95% over 24bp)
AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG101960.4178330626597968Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT87990.36058386802114084Clontech Universal Primer Mix Long (96% over 25bp)
CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT87620.35906760445519215Clontech Universal Primer Mix Long (96% over 25bp)
CAGCTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG85770.3514862866254489Clontech SMARTer II A Oligonucleotide (95% over 24bp)
ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA84520.34636377457832507Clontech Universal Primer Mix Long (96% over 25bp)
TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA66250.2714931384975631Clontech Universal Primer Mix Long (96% over 25bp)
GCTTAGCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTA62680.25686324409097744Clontech SMARTer II A Oligonucleotide (96% over 25bp)
AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG62430.2558387416815527Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCTTACTCTGCGTTGATACCACTGCTTAGCTGCGTTGATACCACTGCTTA60530.24805252336992448Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC58380.2392418026488715Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC56920.23325870857783082Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT55720.22834109701259198Clontech Universal Primer Mix Long (96% over 28bp)
GAGCAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT53690.22002213744806284Clontech Universal Primer Mix Long (96% over 27bp)
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGCTA51660.21170317788353374Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCTTTCTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT48390.1983026863682578Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GTGTTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG41510.17010838006088824Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCTTACTCTGCGTTGATACCACTGCTTGCTCTGCGTTGATACCACTGCTT39700.16269098261665293Clontech Universal Primer Mix Long (96% over 28bp)
GAGGAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT37070.15191321726950438Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT35720.14638090425861064Clontech Universal Primer Mix Long (96% over 25bp)
CTGCTTAGCTGCGTTGATACCACTGCTTAGCTGCGTTGATACCACTGCTT35450.14527444165643189Clontech Universal Primer Mix Long (95% over 21bp)
GAGTTAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT35290.14461876011440003Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCTTGCTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT34870.14289759606656643Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GCTTACATGGGCGTTGATACCACTGCTTAGCTGCGTTGATACCACTGCTT33180.135971959778855Clontech Universal Primer Mix Long (95% over 21bp)
GCTTAGCTGCGTTGATACCACTGCTTAGCTGCGTTGATACCACTGCTTAG32430.13289845255058072Clontech Universal Primer Mix Long (95% over 21bp)
CGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCA31930.13084944773173116Clontech Universal Primer Mix Long (96% over 25bp)
GCTTAGCTGCGTTGATACCACTGCTTAGCTGCGTTGATACCACTGCTTAC29490.12085030421574545Clontech Universal Primer Mix Long (95% over 21bp)
GCTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA28510.11683425477080037Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CAGCTAAGCAGTGGTATCAACGCAGCTAAGCAGTGGTATCAACGCAGCTA28300.11597367274688355No Hit
AAAGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT27330.11199860339831547Clontech SMART CDS Primer II A (100% over 26bp)
TACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACT26620.10908901655554913Clontech Universal Primer Mix Long (96% over 25bp)
ACGCCCATGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG25570.10478610643596513Clontech Universal Primer Mix Long (96% over 25bp)
GCTTACTCTGCGTTGATACCACTGCTTACATGGGCGTTGATACCACTGCT25290.10363866373740939Clontech Universal Primer Mix Long (96% over 25bp)
CTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGC25290.10363866373740939Clontech Universal Primer Mix Long (96% over 25bp)
GAGTAAGCAGTGGTATCAACGCAGCTAAGCAGTGGTATCAACGCAGCTAA24410.1000324152562342No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)