FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001321533

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001321533
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2119930
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATAAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAACG995744.697041883458416Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTTATGTACTCTGCGTTGATACCACTGCTTATGTACTCTGCGTTGATAC688853.2493997443311806Clontech SMART CDS Primer II A (96% over 26bp)
CTTATGTACTCTGCGTTGATACCACTGCTTATGTACTCTGCGTTGATACC306371.445189227946206Clontech SMART CDS Primer II A (96% over 26bp)
GTACACAAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAACG211910.9996084776384127Clontech Universal Primer Mix Long (96% over 25bp)
GAGTACATAAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAA200270.9447010042784431Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATGTACTCTGCGTTGATACCACTGCTTATGTACTCTGCGTTGATACCAC178780.8433297325855099Clontech SMART CDS Primer II A (96% over 26bp)
GTACATAAGCAGTGGTATCAACGCAGAGTACACAAGCAGTGGTATCAACG162540.7667234295472021Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCGTTGATACCACTGCTTATGTACTCTGCGTTGATACCACTGCTTATGTA148560.7007778558725996Clontech SMART CDS Primer II A (96% over 26bp)
GTATCAACGCAGAGTACATAAGCAGTGGTATCAACGCAGAGTACATAAGC141930.6695032383144726Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATAAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAACGCAG138280.6522856886783998Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTTATGTACTCTGCGTTGATACCACTGCTTGTGTACTCTGCGTTGATAC117290.5532729854287641Clontech SMART CDS Primer II A (96% over 26bp)
GCTTGTGTACTCTGCGTTGATACCACTGCTTATGTACTCTGCGTTGATAC115720.5458670805168095Clontech SMART CDS Primer II A (96% over 26bp)
TATCAACGCAGAGTACATAAGCAGTGGTATCAACGCAGAGTACATAAGCA107000.5047336468656984Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACGCAGAGTACATAAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGT92060.43425962177996447Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACTCTGCGTTGATACCACTGCTTATGTACTCTGCGTTGATACCACTGCTT85500.4033152038038992Clontech SMART CDS Primer II A (96% over 26bp)
CTGCTTATGTACTCTGCGTTGATACCACTGCTTATGTACTCTGCGTTGAT83660.39463567193256377Clontech SMART CDS Primer II A (96% over 26bp)
GGTATCAACGCAGAGTACATAAGCAGTGGTATCAACGCAGAGTACATAAG73550.34694541800908524Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACATAAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAACGCA62570.2951512549942687Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAACGCAGAGT53830.25392347860542563Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTTATGTACTCTGCGTTGATACCACTGCTTGTGTACTCTGCGTTGATACC52670.2484515998169751Clontech SMART CDS Primer II A (96% over 26bp)
CAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAACGCAGAGTACA50050.23609270117409537Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACACAAGCAGTGGTATCAACGCAGAGTACACAAGCAGTGGTATCAACG49700.234441703263787Clontech Universal Primer Mix Long (96% over 25bp)
GTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAACGCAGAGTACATA47650.2247715726462666Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTTGTGTACTCTGCGTTGATACCACTGCTTATGTACTCTGCGTTGATACC40730.19212898539102707Clontech SMART CDS Primer II A (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40530.19118555801370798No Hit
GAGTACACAAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAA40230.1897704169477294Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GATACCACTGCTTATGTACTCTGCGTTGATACCACTGCTTATGTACTCTG35920.16943955696650362Clontech SMART CDS Primer II A (96% over 26bp)
GCTTTTGTACTCTGCGTTGATACCACTGCTTATGTACTCTGCGTTGATAC35910.16939238559763764Clontech SMART CDS Primer II A (96% over 26bp)
ATACCACTGCTTATGTACTCTGCGTTGATACCACTGCTTATGTACTCTGC34240.1615147669970235Clontech SMART CDS Primer II A (96% over 26bp)
GAGTACATAAGCAGTGGTATCAACGCAGAGTACACAAGCAGTGGTATCAA33370.15741085790568557Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCACTGCTTATGTACTCTGCGTTGATACCACTGCTTATGTACTCTGCGTT32440.15302392060115194Clontech SMART CDS Primer II A (96% over 26bp)
TATGTACTCTGCGTTGATACCACTGCTTGTGTACTCTGCGTTGATACCAC30970.1460897293778568Clontech SMART CDS Primer II A (96% over 26bp)
GTACAAAAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAACG30690.14476893104961014Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTTGATACCACTGCTTATGTACTCTGCGTTGATACCACTGCTTATGTACT30370.14325944724589965Clontech SMART CDS Primer II A (96% over 26bp)
TGTGTACTCTGCGTTGATACCACTGCTTATGTACTCTGCGTTGATACCAC29480.1390611954168298Clontech SMART CDS Primer II A (96% over 26bp)
ATCAACGCAGAGTACATAAGCAGTGGTATCAACGCAGAGTACATAAGCAG29380.13858948172817026Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGATACCACTGCTTATGTACTCTGCGTTGATACCACTGCTTATGTACTCT27520.12981560711910298Clontech SMART CDS Primer II A (96% over 26bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27420.12934389343044347No Hit
GCGTTGATACCACTGCTTATGTACTCTGCGTTGATACCACTGCTTGTGTA26340.12424938559292052Clontech SMART CDS Primer II A (96% over 26bp)
GCAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAACGCAGAGTAC26020.12273990178921003Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACTCTGCGTTGATACCACTGCTTATGTACTCTGCGTTGATACCACTGC25000.1179284221648828Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TACCACTGCTTATGTACTCTGCGTTGATACCACTGCTTATGTACTCTGCG24450.11533399687725539Clontech SMART CDS Primer II A (96% over 26bp)
GCTTGTGTACTCTGCGTTGATACCACTGCTTGTGTACTCTGCGTTGATAC24190.1141075412867406Clontech SMART CDS Primer II A (96% over 26bp)
TACATAAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAACGC24010.11325845664715345Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATTTACTCTGCGTTGATACCACTGCTTATGTACTCTGCGTTGATACCAC23530.11099423094158768Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GTATCAACGCAGAGTACACAAGCAGTGGTATCAACGCAGAGTACATAAGC23120.10906020481808362Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATAAGCAGTGGTATCAACGCAGAGTACACAAGC23000.10849414839169218Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCGTTGATACCACTGCTTGTGTACTCTGCGTTGATACCACTGCTTATGTA22930.1081639488096305Clontech SMART CDS Primer II A (96% over 26bp)
CATAAGCAGTGGTATCAACGCAGAGTACACAAGCAGTGGTATCAACGCAG22410.10571103762860096Clontech SMARTer II A Oligonucleotide (100% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)