FastQCFastQC Report
Mon 5 Sep 2022
EGAF00001321534

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001321534
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2119930
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATAAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAACG988974.665106866736165Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTTATGTACTCTGCGTTGATACCACTGCTTATGTACTCTGCGTTGATAC728953.4385569334836523Clontech SMART CDS Primer II A (96% over 26bp)
CTTATGTACTCTGCGTTGATACCACTGCTTATGTACTCTGCGTTGATACC287961.3583467378639862Clontech SMART CDS Primer II A (96% over 26bp)
GAGTACATAAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAA227211.0717806720033207Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACACAAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAACG197900.9335213898572123Clontech Universal Primer Mix Long (96% over 25bp)
TATGTACTCTGCGTTGATACCACTGCTTATGTACTCTGCGTTGATACCAC192280.9070110805545466Clontech SMART CDS Primer II A (96% over 26bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN179250.8455467869222096No Hit
GCGTTGATACCACTGCTTATGTACTCTGCGTTGATACCACTGCTTATGTA160540.7572891557740113Clontech SMART CDS Primer II A (96% over 26bp)
GTACATAAGCAGTGGTATCAACGCAGAGTACACAAGCAGTGGTATCAACG157460.7427603741632979Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATAAGCAGTGGTATCAACGCAGAGTACATAAGC144170.6800696249404462Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATAAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAACGCAG127000.5990763845976047Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTTATGTACTCTGCGTTGATACCACTGCTTGTGTACTCTGCGTTGATAC123420.5821890345435934Clontech SMART CDS Primer II A (96% over 26bp)
GCTTGTGTACTCTGCGTTGATACCACTGCTTATGTACTCTGCGTTGATAC110530.5213851400753798Clontech SMART CDS Primer II A (96% over 26bp)
TATCAACGCAGAGTACATAAGCAGTGGTATCAACGCAGAGTACATAAGCA105280.4966201714207545Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACGCAGAGTACATAAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGT87190.4112871651422453Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTGCTTATGTACTCTGCGTTGATACCACTGCTTATGTACTCTGCGTTGAT85380.4027491473775078Clontech SMART CDS Primer II A (96% over 26bp)
ACTCTGCGTTGATACCACTGCTTATGTACTCTGCGTTGATACCACTGCTT83330.3930790167599873Clontech SMART CDS Primer II A (96% over 26bp)
GGTATCAACGCAGAGTACATAAGCAGTGGTATCAACGCAGAGTACATAAG75020.3538796092323803Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACATAAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAACGCA57110.2693956875934583Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAACGCAGAGTACATA52950.24977239814522179Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTTATGTACTCTGCGTTGATACCACTGCTTGTGTACTCTGCGTTGATACC50090.2362813866495592Clontech SMART CDS Primer II A (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAACGCAGAGT49450.23326241904213818Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACACAAGCAGTGGTATCAACGCAGAGTACACAAGCAGTGGTATCAACG49070.23146990702523196Clontech Universal Primer Mix Long (96% over 25bp)
CAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAACGCAGAGTACA46440.21906383701348628Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTTGTGTACTCTGCGTTGATACCACTGCTTATGTACTCTGCGTTGATACC45510.21467689970895268Clontech SMART CDS Primer II A (96% over 26bp)
GAGTACACAAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAA43320.20434636992730892Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACATAAGCAGTGGTATCAACGCAGAGTACACAAGCAGTGGTATCAA37580.1772700041982518Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36930.17420386522196488No Hit
GTTGATACCACTGCTTATGTACTCTGCGTTGATACCACTGCTTATGTACT35560.1677413876873293Clontech SMART CDS Primer II A (96% over 26bp)
GATACCACTGCTTATGTACTCTGCGTTGATACCACTGCTTATGTACTCTG34370.16212799479228088Clontech SMART CDS Primer II A (96% over 26bp)
ATACCACTGCTTATGTACTCTGCGTTGATACCACTGCTTATGTACTCTGC33570.15835428528300463Clontech SMART CDS Primer II A (96% over 26bp)
TGTGTACTCTGCGTTGATACCACTGCTTATGTACTCTGCGTTGATACCAC33550.1582599425452727Clontech SMART CDS Primer II A (96% over 26bp)
TATGTACTCTGCGTTGATACCACTGCTTGTGTACTCTGCGTTGATACCAC32240.15208049322383285Clontech SMART CDS Primer II A (96% over 26bp)
CCACTGCTTATGTACTCTGCGTTGATACCACTGCTTATGTACTCTGCGTT31370.14797658413249495Clontech SMART CDS Primer II A (96% over 26bp)
GTACAAAAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAACG29830.14071219332713816Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGATACCACTGCTTATGTACTCTGCGTTGATACCACTGCTTATGTACTCT28980.13670262697353214Clontech SMART CDS Primer II A (96% over 26bp)
ATCAACGCAGAGTACATAAGCAGTGGTATCAACGCAGAGTACATAAGCAG28620.13500445769435784Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCGTTGATACCACTGCTTATGTACTCTGCGTTGATACCACTGCTTGTGTA28300.13349497389064732Clontech SMART CDS Primer II A (96% over 26bp)
GCAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAACGCAGAGTAC27650.1304288349143604Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26430.1246739279127141No Hit
GTACTCTGCGTTGATACCACTGCTTATGTACTCTGCGTTGATACCACTGC25930.12231535946941643Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTTTTGTACTCTGCGTTGATACCACTGCTTATGTACTCTGCGTTGATAC25580.12066436155910808Clontech SMART CDS Primer II A (96% over 26bp)
GTATCAACGCAGAGTACACAAGCAGTGGTATCAACGCAGAGTACATAAGC25100.11840013585354234Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCGTTGATACCACTGCTTGTGTACTCTGCGTTGATACCACTGCTTATGTA24490.11552268235271919Clontech SMART CDS Primer II A (96% over 26bp)
GTATCAACGCAGAGTACATAAGCAGTGGTATCAACGCAGAGTACACAAGC24000.1132112852782875Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TACCACTGCTTATGTACTCTGCGTTGATACCACTGCTTATGTACTCTGCG23360.1101923176708665Clontech SMART CDS Primer II A (96% over 26bp)
GCTTGTGTACTCTGCGTTGATACCACTGCTTGTGTACTCTGCGTTGATAC23340.11009797493313458Clontech SMART CDS Primer II A (96% over 26bp)
TACATAAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAACGC22380.10556952352200309Clontech SMARTer II A Oligonucleotide (100% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)