FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001321539

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001321539
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3726065
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTACTGCGTTGATACCACTGCTTACTGCGTTGATACCACTGCTTACTG207540.5569951141485723Clontech Universal Primer Mix Long (95% over 21bp)
GGTATCAACGCAGTAAGCAGTGGTATCAACGCAGTAAGCAGTGGTATCAA159500.42806553294158844No Hit
GCGTTGATACCACTGCTTACTGCGTTGATACCACTGCTTACTGCGTTGAT103300.277236172745242Clontech Universal Primer Mix Long (95% over 21bp)
GCAGTAAGCAGTGGTATCAACGCAGTAAGCAGTGGTATCAACGCAGTAAG100380.2693994871264994No Hit
AAGCAGTGGTATCAACGCAGTAAGCAGTGGTATCAACGCAGTAAGCAGTG99580.26725244997067954Clontech Universal Primer Mix Long (95% over 22bp)
CAGTAAGCAGTGGTATCAACGCAGTAAGCAGTGGTATCAACGCAGTAAGC98080.26322675530351725Clontech Universal Primer Mix Long (95% over 22bp)
GTTGATACCACTGCTTACTGCGTTGATACCACTGCTTACTGCGTTGATAC91430.2453795089457645Clontech Universal Primer Mix Long (95% over 21bp)
CCACTGCTTACTGCGTTGATACCACTGCTTACTGCGTTGATACCACTGCT88680.23799906872263368Clontech Universal Primer Mix Long (95% over 21bp)
GTATCAACGCAGTAAGCAGTGGTATCAACGCAGTAAGCAGTGGTATCAAC88420.23730128164699218Clontech Universal Primer Mix Long (95% over 22bp)
GTAAGCAGTGGTATCAACGCAGTAAGCAGTGGTATCAACGCAGTAAGCAG87670.23528843431341104Clontech Universal Primer Mix Long (95% over 22bp)
GTGGTATCAACGCAGTAAGCAGTGGTATCAACGCAGTAAGCAGTGGTATC64080.17197767618117235No Hit
TACCACTGCTTACTGCGTTGATACCACTGCTTACTGCGTTGATACCACTG61140.16408731463353432Clontech Universal Primer Mix Long (95% over 21bp)
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT57340.15388888814338988Clontech SMARTer II A Oligonucleotide (95% over 24bp)
TATCAACGCAGTAAGCAGTGGTATCAACGCAGTAAGCAGTGGTATCAACG56520.1516881750586745No Hit
ACGCAGTAAGCAGTGGTATCAACGCAGTAAGCAGTGGTATCAACGCAGTA55190.14811872578712396Clontech Universal Primer Mix Long (95% over 22bp)
GATACCACTGCTTACTGCGTTGATACCACTGCTTACTGCGTTGATACCAC54710.14683050349363203Clontech Universal Primer Mix Long (95% over 21bp)
GCAGTGGTATCAACGCAGTAAGCAGTGGTATCAACGCAGTAAGCAGTGGT51330.13775927151029305No Hit
CTGCTTACTGCGTTGATACCACTGCTTACTGCGTTGATACCACTGCTTAC48890.1312108081850424Clontech Universal Primer Mix Long (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48530.13024464146492346No Hit
ATACCACTGCTTACTGCGTTGATACCACTGCTTACTGCGTTGATACCACT44420.11921423807689882Clontech Universal Primer Mix Long (95% over 21bp)
CAGTGGTATCAACGCAGTAAGCAGTGGTATCAACGCAGTAAGCAGTGGTA43190.11591316844982577Clontech Universal Primer Mix Long (95% over 22bp)
TGATACCACTGCTTACTGCGTTGATACCACTGCTTACTGCGTTGATACCA42800.11486648783636356Clontech Universal Primer Mix Long (95% over 21bp)
TTACTGCGTTGATACCACTGCTTACTGCGTTGATACCACTGCTTACTGCG39390.10571474195968132Clontech Universal Primer Mix Long (95% over 21bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)