FastQCFastQC Report
Mon 12 Sep 2022
EGAF00001321540

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001321540
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3726065
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN305520.819953489807612No Hit
GCTTACTGCGTTGATACCACTGCTTACTGCGTTGATACCACTGCTTACTG230080.6174878860137973Clontech Universal Primer Mix Long (95% over 21bp)
GGTATCAACGCAGTAAGCAGTGGTATCAACGCAGTAAGCAGTGGTATCAA157620.4230199956254118No Hit
GCGTTGATACCACTGCTTACTGCGTTGATACCACTGCTTACTGCGTTGAT114380.3069726373533473Clontech Universal Primer Mix Long (95% over 21bp)
GTTGATACCACTGCTTACTGCGTTGATACCACTGCTTACTGCGTTGATAC107980.28979634010678823Clontech Universal Primer Mix Long (95% over 21bp)
GCAGTAAGCAGTGGTATCAACGCAGTAAGCAGTGGTATCAACGCAGTAAG104580.28067143219455376No Hit
CCACTGCTTACTGCGTTGATACCACTGCTTACTGCGTTGATACCACTGCT95020.2550143381825062Clontech Universal Primer Mix Long (95% over 21bp)
AAGCAGTGGTATCAACGCAGTAAGCAGTGGTATCAACGCAGTAAGCAGTG92720.24884160635952404Clontech Universal Primer Mix Long (95% over 22bp)
CAGTAAGCAGTGGTATCAACGCAGTAAGCAGTGGTATCAACGCAGTAAGC89940.24138065224304997Clontech Universal Primer Mix Long (95% over 22bp)
GTATCAACGCAGTAAGCAGTGGTATCAACGCAGTAAGCAGTGGTATCAAC88520.2375696612914697Clontech Universal Primer Mix Long (95% over 22bp)
GTAAGCAGTGGTATCAACGCAGTAAGCAGTGGTATCAACGCAGTAAGCAG79880.21438166000861497Clontech Universal Primer Mix Long (95% over 22bp)
GTGGTATCAACGCAGTAAGCAGTGGTATCAACGCAGTAAGCAGTGGTATC68960.18507460283167362No Hit
TACCACTGCTTACTGCGTTGATACCACTGCTTACTGCGTTGATACCACTG63890.17146775485666516Clontech Universal Primer Mix Long (95% over 21bp)
GATACCACTGCTTACTGCGTTGATACCACTGCTTACTGCGTTGATACCAC56050.15042679072963033Clontech Universal Primer Mix Long (95% over 21bp)
GCAGTGGTATCAACGCAGTAAGCAGTGGTATCAACGCAGTAAGCAGTGGT55460.14884335082721314No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT55360.1485749711827357Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CTGCTTACTGCGTTGATACCACTGCTTACTGCGTTGATACCACTGCTTAC52320.14041622999062012Clontech Universal Primer Mix Long (95% over 21bp)
TATCAACGCAGTAAGCAGTGGTATCAACGCAGTAAGCAGTGGTATCAACG52270.1402820401683814No Hit
ACGCAGTAAGCAGTGGTATCAACGCAGTAAGCAGTGGTATCAACGCAGTA52270.1402820401683814Clontech Universal Primer Mix Long (95% over 22bp)
ATACCACTGCTTACTGCGTTGATACCACTGCTTACTGCGTTGATACCACT45020.12082451594376373Clontech Universal Primer Mix Long (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44340.11899953436131684No Hit
TGATACCACTGCTTACTGCGTTGATACCACTGCTTACTGCGTTGATACCA44290.11886534453907809Clontech Universal Primer Mix Long (95% over 21bp)
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT40940.10987462644908234Clontech Universal Primer Mix Long (96% over 25bp)
TTACTGCGTTGATACCACTGCTTACTGCGTTGATACCACTGCTTACTGCG39020.10472173727511463Clontech Universal Primer Mix Long (95% over 21bp)
CAGTGGTATCAACGCAGTAAGCAGTGGTATCAACGCAGTAAGCAGTGGTA38970.10458754745287589Clontech Universal Primer Mix Long (95% over 22bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)