FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001321548

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001321548
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3224608
Sequences flagged as poor quality0
Sequence length100
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT1686915.231364556560053Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT1291884.00631642667884Clontech Universal Primer Mix Long (96% over 25bp)
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC766282.376350861872203Clontech Universal Primer Mix Long (96% over 25bp)
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT632001.9599281525072194Clontech Universal Primer Mix Long (96% over 25bp)
GCTTATACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC465321.4430281138048406Clontech Universal Primer Mix Long (96% over 25bp)
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT461971.4326392541356965Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACT382971.1876482350722941Clontech SMARTer II A Oligonucleotide (96% over 25bp)
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT379521.1769492601891454Clontech Universal Primer Mix Long (96% over 25bp)
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA359471.114771159781282Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT349101.0826122120890354Clontech SMARTer II A Oligonucleotide (96% over 25bp)
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC341571.0592605364745111Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA338881.050918437217795Clontech Universal Primer Mix Long (96% over 25bp)
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT315340.9779173158411814Clontech SMART CDS Primer II A (96% over 26bp)
GCTTGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC280060.8685086683404619Clontech SMART CDS Primer II A (96% over 26bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN278990.8651904355506158No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC277360.8601355575623455Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGA273010.8466455457531582Clontech Universal Primer Mix Long (95% over 24bp)
GTATAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT262670.8145796326251129Clontech Universal Primer Mix Long (95% over 24bp)
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC247680.7680933620458673Clontech Universal Primer Mix Long (96% over 25bp)
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA243290.7544793041510782Clontech Universal Primer Mix Long (96% over 25bp)
GAGTATAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA219460.6805788486538519Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA218300.6769815121714019Clontech Universal Primer Mix Long (96% over 25bp)
GTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA216710.6720506802687334Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCA210840.6538469172066806Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG207970.6449466105647571Clontech Universal Primer Mix Long (96% over 25bp)
GAGTACAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA206200.6394575712768807Clontech Universal Primer Mix Long (96% over 25bp)
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC182230.5651229544800485Clontech Universal Primer Mix Long (96% over 25bp)
GAGTAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA178180.552563288312874Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA172440.5347626750290267Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT172320.5343905367722216Clontech Universal Primer Mix Long (100% over 24bp)
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC165460.5131166330915262Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GAGTAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGA159510.49466477785826996Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCTTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGC156680.485888517301948Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA151390.4694834224811202Clontech Universal Primer Mix Long (96% over 25bp)
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC143430.4447982514463773Clontech Universal Primer Mix Long (96% over 25bp)
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG138830.43053295160217925Clontech Universal Primer Mix Long (96% over 25bp)
GCTTGTACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACT138100.4282691105399478Clontech SMART CDS Primer II A (96% over 26bp)
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT136550.42346232472288103Clontech Universal Primer Mix Long (96% over 25bp)
GAGTACAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCA133850.4150892139447648Clontech Universal Primer Mix Long (96% over 25bp)
GCTTACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACTGC132490.4108716470343061Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GTACAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGA131490.4077704948942631Clontech Universal Primer Mix Long (100% over 24bp)
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC129540.4017232482211791Clontech Universal Primer Mix Long (96% over 25bp)
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG117670.3649125723188679Clontech Universal Primer Mix Long (96% over 25bp)
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT114980.35657047306215206Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA101860.315883356984787Clontech SMART CDS Primer II A (96% over 26bp)
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG100460.3115417439887267Clontech SMART CDS Primer II A (96% over 26bp)
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT93820.2909500937788407Clontech SMART CDS Primer II A (96% over 26bp)
ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG89390.2772119897984499Clontech Universal Primer Mix Long (96% over 25bp)
CTGCTTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT84220.26117903323442726Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG84040.26062082584921953Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACTG80210.24874341315285456Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CTGCTTACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACT78190.24247908582996758Clontech SMARTer II A Oligonucleotide (96% over 25bp)
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC75080.23283450267443362Clontech SMART CDS Primer II A (96% over 26bp)
AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG73600.22824479750716986Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CTTATACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT72770.22567084123093412Clontech Universal Primer Mix Long (96% over 25bp)
CTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG70430.21841414522323332Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT66740.2069708938264744Clontech Universal Primer Mix Long (96% over 25bp)
AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA63690.19751237979934305Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT62650.19428718157369826Clontech Universal Primer Mix Long (96% over 28bp)
TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG59840.18557294406017721Clontech Universal Primer Mix Long (96% over 25bp)
CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT58760.1822236997489307Clontech Universal Primer Mix Long (96% over 25bp)
CTGCTTGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT52000.16125991128223957Clontech SMART CDS Primer II A (96% over 26bp)
GCTTACTCTGCGTTGATACCACTGCTTGCTCTGCGTTGATACCACTGCTT51270.15899607022000814Clontech Universal Primer Mix Long (96% over 28bp)
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC50840.15766257479978962Clontech SMARTer II A Oligonucleotide (95% over 24bp)
TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA50020.1551196300449543Clontech Universal Primer Mix Long (96% over 25bp)
ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA48650.1508710516130953Clontech Universal Primer Mix Long (96% over 25bp)
CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA48430.15018879814228583Clontech Universal Primer Mix Long (96% over 25bp)
GCGTTGATACCACTGCTTATACTCTGCGTTGATACCACTGCTTATACTCT47190.1463433694886324Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CTTGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT47180.146312357967232Clontech SMART CDS Primer II A (96% over 26bp)
CTGCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCA46780.14507189711121476Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT45330.1405752265081523Clontech Universal Primer Mix Long (96% over 25bp)
CTGCTTATACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT44690.13859048913852473Clontech Universal Primer Mix Long (96% over 25bp)
GCTTAGGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT44340.1375050858895097Clontech SMART CDS Primer II A (96% over 26bp)
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTGTACTCTGC43190.13393876092846013Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GCTTAGGTACTCTGCGTTGATACCACTGCTTAGGTACTCTGCGTTGATAC43140.13378370332145798Clontech SMART CDS Primer II A (96% over 26bp)
GAGCAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT42370.1313958161736248Clontech Universal Primer Mix Long (96% over 27bp)
ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG41140.12758139904137183Clontech SMART CDS Primer II A (96% over 26bp)
CTGCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA40880.12677509948496066Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTATACTCTGC40050.12420114320872491Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GCGTTGATACCACTGCTTATACTCTGCGTTGATACCACTGCTTACTCTGC40010.12407709712312319Clontech Universal Primer Mix Long (96% over 25bp)
CTGCTTGTACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCA39910.12376698190911888Clontech SMART CDS Primer II A (96% over 26bp)
GTACCTAAGCAGTGGTATCAACGCAGAGTACCTAAGCAGTGGTATCAACG39900.12373597038771844Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTTGTACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACTG39450.12234045192469907Clontech SMART CDS Primer II A (96% over 26bp)
GCGTTGATACCACTGCTTATACTCTGCGTTGATACCACTGCTTGTACTCT39370.12209235975349562Clontech SMARTer II A Oligonucleotide (96% over 25bp)
AAAGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT39130.12134808323988529Clontech SMART CDS Primer II A (100% over 26bp)
ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA38880.12057279520487452Clontech Universal Primer Mix Long (96% over 25bp)
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTACTCTGC38730.12010762238386806Clontech SMART CDS Primer II A (96% over 26bp)
AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG37010.11477364070299398Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG35190.1091295438081156Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTATACTCT34730.10770301382369578Clontech SMART CDS Primer II A (96% over 26bp)
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGT34590.10726885252408974Clontech Universal Primer Mix Long (100% over 24bp)
GTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTG34450.10683469122448372Clontech Universal Primer Mix Long (95% over 24bp)
GCTTTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC33940.10525310363306176Clontech Universal Primer Mix Long (96% over 25bp)
TGCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCAC33810.10484995385485615Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GCTTGCTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT33370.1034854469132372Clontech SMARTer II A Oligonucleotide (96% over 25bp)
ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT32460.10066339846579803Clontech SMART CDS Primer II A (96% over 26bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)