FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001321562

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001321562
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1634288
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT593193.6296540144699097Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT355092.1727504576916674Clontech Universal Primer Mix Long (96% over 25bp)
GCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACT284701.7420430181216529Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT247341.513441939242043Clontech Universal Primer Mix Long (96% over 25bp)
GTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGA246511.5083632750163987Clontech Universal Primer Mix Long (95% over 24bp)
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC240121.4692636793514975Clontech Universal Primer Mix Long (96% over 25bp)
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT186331.1401295242943716Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GAGTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCA180721.1058026492270638Clontech SMARTer II A Oligonucleotide (95% over 24bp)
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT148800.9104882370793886Clontech Universal Primer Mix Long (96% over 25bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN139700.8548064967741303No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC136240.8336351977130101Clontech SMARTer II A Oligonucleotide (95% over 24bp)
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC116170.710829425413391Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA102370.6263889840713509Clontech Universal Primer Mix Long (96% over 25bp)
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC100650.6158645232664011Clontech Universal Primer Mix Long (96% over 25bp)
GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT93890.5745009447539234Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA88220.5398069373329548Clontech Universal Primer Mix Long (96% over 25bp)
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG85770.5248156995584621Clontech Universal Primer Mix Long (96% over 25bp)
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA76340.4671147313080681Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACTG74360.4549993636372537Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC72820.4455762998932869Clontech Universal Primer Mix Long (96% over 25bp)
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC72820.4455762998932869Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA70580.43187002535660785Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTTATACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC64890.3970536404844189Clontech Universal Primer Mix Long (96% over 25bp)
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA62410.38187883653309573Clontech Universal Primer Mix Long (96% over 25bp)
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC58900.3604015938439247Clontech Universal Primer Mix Long (96% over 25bp)
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA57420.351345662453619Clontech Universal Primer Mix Long (96% over 25bp)
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG55830.34161665508160133Clontech Universal Primer Mix Long (96% over 25bp)
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT53550.3276656256424816Clontech Universal Primer Mix Long (96% over 25bp)
GAGTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA53260.32589115259978657Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATAAGCAGTGGTATCAACGCAGAGTACATAAGCAGTGGTATCAACG53190.32546283152051536Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG51680.3162233339533791Clontech Universal Primer Mix Long (96% over 25bp)
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC49580.30337370157524257Clontech Universal Primer Mix Long (96% over 25bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49380.3021499270630391No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT48940.2994576231361914Clontech SMART CDS Primer II A (96% over 26bp)
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT46620.285261838794631Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GTACAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGA45460.27816394662385086Clontech Universal Primer Mix Long (100% over 24bp)
GCGTTGATACCACTGCTTATACTCTGCGTTGATACCACTGCTTATACTCT44550.272595772593325Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CTGCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCA44410.2717391304347826Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GTATAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT42360.25919544168469694Clontech Universal Primer Mix Long (95% over 24bp)
GAGTACAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCA40830.24983356666634032Clontech Universal Primer Mix Long (96% over 25bp)
GTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTG36870.22560283132471143Clontech Universal Primer Mix Long (95% over 24bp)
GCTTATGTACTCTGCGTTGATACCACTGCTTATGTACTCTGCGTTGATAC36070.22070773327589754Clontech SMART CDS Primer II A (96% over 26bp)
GCTTGTACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACT35660.2181989955258804Clontech SMART CDS Primer II A (96% over 26bp)
ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG35160.21513955924537168Clontech Universal Primer Mix Long (96% over 25bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34410.21055040482460863No Hit
TGCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCAC34260.209632573940456Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GAGTATAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA33090.20247349304406567Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GAGTAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGA26790.16392459590965608Clontech SMARTer II A Oligonucleotide (95% over 24bp)
AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG26650.16306795375111363Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT25400.15541936304984189Clontech Universal Primer Mix Long (96% over 25bp)
AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA24290.1486274145071126Clontech SMARTer II A Oligonucleotide (95% over 24bp)
TATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGG23560.14416063753756989Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT23350.14287567429975623Clontech Universal Primer Mix Long (96% over 25bp)
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA22920.14024455909851874Clontech SMART CDS Primer II A (96% over 26bp)
CTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG22850.13981623801924753Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGT22050.13492113997043362Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCTTACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACTGC22000.13461519634238273Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG21290.1302707968240604Clontech SMART CDS Primer II A (96% over 26bp)
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG21150.12941415466551795Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20520.12555926495207698No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT20310.12427430171426333Clontech SMART CDS Primer II A (96% over 26bp)
ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA19600.11992990219594098Clontech Universal Primer Mix Long (96% over 25bp)
ACGCAGAGTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCA19000.11625857865933055Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCTTGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC17990.11007851737270297Clontech SMART CDS Primer II A (96% over 26bp)
GCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC17840.10916068648855037Clontech Universal Primer Mix Long (96% over 25bp)
TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA17620.10781453452512654Clontech Universal Primer Mix Long (96% over 25bp)
CTTATGTACTCTGCGTTGATACCACTGCTTATGTACTCTGCGTTGATACC16770.10261349284826175Clontech SMART CDS Primer II A (96% over 26bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)