FastQCFastQC Report
Sat 10 Sep 2022
EGAF00001321583

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001321583
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2297657
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTAAGCAGTGGTATCAACGCAGAGTACATGGGTAAGCAGTGG427171.8591547824588266Clontech Universal Primer Mix Long (96% over 27bp)
CCCATGTACTCTGCGTTGATACCACTGCTTACCCATGTACTCTGCGTTGA374281.6289637661321947Clontech Universal Primer Mix Long (96% over 27bp)
GGTAAGCAGTGGTATCAACGCAGAGTACATGGGTAAGCAGTGGTATCAAC203040.8836828125346821Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGTAAGCAGTGGTATCAACGCAGAGTACATGGGTAAGCAGT122750.5342398800169043Clontech Universal Primer Mix Long (96% over 27bp)
GTATCAACGCAGAGTACATGGGTAAGCAGTGGTATCAACGCAGAGTACAT121850.5303228462733993Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTGCTTACCCATGTACTCTGCGTTGATACCACTGCTTACCCATGTACTCT121070.5269280836956952Clontech Universal Primer Mix Long (96% over 27bp)
GCTTACCCATGTACTCTGCGTTGATACCACTGCTTACCCATGTACTCTGC109220.47535380607288213Clontech Universal Primer Mix Long (96% over 27bp)
GCGTTGATACCACTGCTTACCCATGTACTCTGCGTTGATACCACTGCTTA100360.4367927849979349Clontech SMART CDS Primer II A (96% over 26bp)
TATCAACGCAGAGTACATGGGTAAGCAGTGGTATCAACGCAGAGTACATG90130.3922691681134303Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGGGTAAGCAGTGGTATCAACGCAGAGTACATGGGTAAGCAGTGGTATCA89590.38991894786732745Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACGCAGAGTACATGGGTAAGCAGTGGTATCAACGCAGAGTACATGGGTAA86200.3751647874334594Clontech Universal Primer Mix Long (96% over 27bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT80270.3493558873234778Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA75880.33024946717460435Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGTACTCTGCGTTGATACCACTGCTTACCCATGTACTCTGCGTTGATA73890.3215884703417438Clontech Universal Primer Mix Long (96% over 27bp)
ACTCTGCGTTGATACCACTGCTTACCCATGTACTCTGCGTTGATACCACT70250.3057462449791244Clontech Universal Primer Mix Long (96% over 27bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60040.26130967328892No Hit
GGTATCAACGCAGAGTACATGGGTAAGCAGTGGTATCAACGCAGAGTACA59940.26087444731741943Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT51960.22614341479167693Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCAGTGGTATCAACGCAGAGTACATGGGTAAGCAGTGGTATCAACGCAGA45440.19776668144984216Clontech SMARTer II A Oligonucleotide (100% over 23bp)
TACCACTGCTTACCCATGTACTCTGCGTTGATACCACTGCTTACCCATGT44980.19576464198093974Clontech Universal Primer Mix Long (96% over 27bp)
GTGGTATCAACGCAGAGTACATGGGTAAGCAGTGGTATCAACGCAGAGTA42450.18475342490197624Clontech SMARTer II A Oligonucleotide (100% over 24bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41520.1807058233670213No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG37330.16246985516114895Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG33590.14619240382702903Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGTAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT31780.13831481374286936Clontech Universal Primer Mix Long (96% over 27bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG28910.12582382836080408Clontech Universal Primer Mix Long (96% over 26bp)
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT28720.12499689901495306Clontech Universal Primer Mix Long (96% over 25bp)
CAGTGGTATCAACGCAGAGTACATGGGTAAGCAGTGGTATCAACGCAGAG28640.12464871823775264Clontech Universal Primer Mix Long (100% over 22bp)
GCTTAGCGTTGATACCACTGCTTAGCGTTGATACCACTGCTTAGCGTTGA28280.1230819047403507No Hit
GATACCACTGCTTACCCATGTACTCTGCGTTGATACCACTGCTTACCCAT27820.12107986527144826Clontech Universal Primer Mix Long (96% over 27bp)
GCTTAGCGTTGATACCACTGCTTACCCATGTACTCTGCGTTGATACCACT26910.11711930893079342Clontech SMART CDS Primer II A (95% over 22bp)
GGGTAAGCAGTGGTATCAACGCAGAGTACATGGGTAAGCAGTGGTATCAA26550.11555249543339148Clontech Universal Primer Mix Long (96% over 27bp)
CCCATGTACTCTGCGTTGATACCACTGCTTAGCGTTGATACCACTGCTTA26110.11363750115878915Clontech SMART CDS Primer II A (96% over 26bp)
ATACCACTGCTTACCCATGTACTCTGCGTTGATACCACTGCTTACCCATG25990.11311522999298851Clontech Universal Primer Mix Long (96% over 27bp)
ATCAACGCAGAGTACATGGGTAAGCAGTGGTATCAACGCAGAGTACATGG23710.10319207784277637Clontech Universal Primer Mix Long (96% over 27bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGTAAGCAGTGGTATCAACGCA23390.10179935473397464Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23110.10058072201377316No Hit
GTACTCTGCGTTGATACCACTGCTTACCCATGTACTCTGCGTTGATACCA23050.10031958643087284Clontech Universal Primer Mix Long (96% over 27bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)