FastQCFastQC Report
Sat 10 Sep 2022
EGAF00001321584

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001321584
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2297657
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTAAGCAGTGGTATCAACGCAGAGTACATGGGTAAGCAGTGG411571.7912595309047434Clontech Universal Primer Mix Long (96% over 27bp)
CCCATGTACTCTGCGTTGATACCACTGCTTACCCATGTACTCTGCGTTGA385841.6792758884376564Clontech Universal Primer Mix Long (96% over 27bp)
GGTAAGCAGTGGTATCAACGCAGAGTACATGGGTAAGCAGTGGTATCAAC192850.8393332860387779Clontech Universal Primer Mix Long (96% over 26bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN178590.7772700625028017No Hit
CTGCTTACCCATGTACTCTGCGTTGATACCACTGCTTACCCATGTACTCT131480.5722351073289007Clontech Universal Primer Mix Long (96% over 27bp)
GAGTACATGGGTAAGCAGTGGTATCAACGCAGAGTACATGGGTAAGCAGT130990.570102500068548Clontech Universal Primer Mix Long (96% over 27bp)
GCTTACCCATGTACTCTGCGTTGATACCACTGCTTACCCATGTACTCTGC119870.5217053720376889Clontech Universal Primer Mix Long (96% over 27bp)
GTATCAACGCAGAGTACATGGGTAAGCAGTGGTATCAACGCAGAGTACAT119410.5197033325687864Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCGTTGATACCACTGCTTACCCATGTACTCTGCGTTGATACCACTGCTTA110270.4799236787736376Clontech SMART CDS Primer II A (96% over 26bp)
TGGGTAAGCAGTGGTATCAACGCAGAGTACATGGGTAAGCAGTGGTATCA93410.40654457997864785Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCAACGCAGAGTACATGGGTAAGCAGTGGTATCAACGCAGAGTACATG85170.3706819599270039Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT82000.356885296630437Clontech Universal Primer Mix Long (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTACCCATGTACTCTGCGTTGATA81970.35675472883898685Clontech Universal Primer Mix Long (96% over 27bp)
ACGCAGAGTACATGGGTAAGCAGTGGTATCAACGCAGAGTACATGGGTAA80130.348746570963377Clontech Universal Primer Mix Long (96% over 27bp)
ACTCTGCGTTGATACCACTGCTTACCCATGTACTCTGCGTTGATACCACT72250.31445076440913505Clontech Universal Primer Mix Long (96% over 27bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA72160.31405906103478454Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTATCAACGCAGAGTACATGGGTAAGCAGTGGTATCAACGCAGAGTACA60690.26413864210367344Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54560.23745929005069077No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT52530.22862420282922993Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCAGTGGTATCAACGCAGAGTACATGGGTAAGCAGTGGTATCAACGCAGA49700.21630730783576488Clontech SMARTer II A Oligonucleotide (100% over 23bp)
TACCACTGCTTACCCATGTACTCTGCGTTGATACCACTGCTTACCCATGT45800.19933349494724412Clontech Universal Primer Mix Long (96% over 27bp)
GTGGTATCAACGCAGAGTACATGGGTAAGCAGTGGTATCAACGCAGAGTA44350.19302271836048635Clontech SMARTer II A Oligonucleotide (100% over 24bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38690.1683889283735562No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG36990.16099008685804714Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG36870.1604678156922465Clontech Universal Primer Mix Long (96% over 26bp)
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT34370.14958716640473316Clontech Universal Primer Mix Long (96% over 25bp)
GCTTAGCGTTGATACCACTGCTTAGCGTTGATACCACTGCTTAGCGTTGA30230.1315688111846111No Hit
GCTTAGCGTTGATACCACTGCTTACCCATGTACTCTGCGTTGATACCACT29950.1303501784644096Clontech SMART CDS Primer II A (95% over 22bp)
GTACATGGGTAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT29350.12773882263540642Clontech Universal Primer Mix Long (96% over 27bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG27320.11890373541394561Clontech Universal Primer Mix Long (96% over 26bp)
GATACCACTGCTTACCCATGTACTCTGCGTTGATACCACTGCTTACCCAT26810.11668408295929288Clontech Universal Primer Mix Long (96% over 27bp)
GGGTAAGCAGTGGTATCAACGCAGAGTACATGGGTAAGCAGTGGTATCAA26240.11420329492173983Clontech Universal Primer Mix Long (96% over 27bp)
CCCATGTACTCTGCGTTGATACCACTGCTTAGCGTTGATACCACTGCTTA25520.11106966792693601Clontech SMART CDS Primer II A (96% over 26bp)
ATACCACTGCTTACCCATGTACTCTGCGTTGATACCACTGCTTACCCATG25380.11046035156683526Clontech Universal Primer Mix Long (96% over 27bp)
CAGTGGTATCAACGCAGAGTACATGGGTAAGCAGTGGTATCAACGCAGAG25320.11019921598393494Clontech Universal Primer Mix Long (100% over 22bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC24800.10793604093213217Clontech Universal Primer Mix Long (96% over 26bp)
GTACTCTGCGTTGATACCACTGCTTACCCATGTACTCTGCGTTGATACCA24440.10636922743473025Clontech Universal Primer Mix Long (96% over 27bp)
TGATACCACTGCTTACCCATGTACTCTGCGTTGATACCACTGCTTACCCA23330.10153821915107432Clontech Universal Primer Mix Long (96% over 27bp)
GTTGATACCACTGCTTACCCATGTACTCTGCGTTGATACCACTGCTTACC23220.10105947058242375Clontech Universal Primer Mix Long (96% over 26bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)