FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001321591

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001321591
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2967618
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT51220.17259633820794995Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATG40430.1362372111235341No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38400.12939670806687384No Hit
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG36900.12434214915801158No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATAT36240.12211814323811218No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)