FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001321595

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001321595
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3785882
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT2198295.806546532617762Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT1381553.6492156913501264Clontech Universal Primer Mix Long (96% over 25bp)
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC831802.197110211041971Clontech Universal Primer Mix Long (96% over 25bp)
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT723611.9113379656312584Clontech Universal Primer Mix Long (96% over 25bp)
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT701811.8537556109778381Clontech SMARTer II A Oligonucleotide (95% over 24bp)
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC548281.4482226334576724Clontech SMARTer II A Oligonucleotide (95% over 24bp)
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT438201.157458156382053Clontech Universal Primer Mix Long (96% over 25bp)
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC426731.127161385378625Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA355980.9402828719965388Clontech Universal Primer Mix Long (96% over 25bp)
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA353110.9327020757646435Clontech Universal Primer Mix Long (96% over 25bp)
GTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGA293490.7752222599647849Clontech Universal Primer Mix Long (95% over 24bp)
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC289290.764128411820548Clontech Universal Primer Mix Long (96% over 25bp)
GCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACT285260.7534836003869111Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG276790.7311110066293667Clontech Universal Primer Mix Long (96% over 25bp)
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC271940.7183002534151883Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCTTATACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC233880.6177688580890793Clontech Universal Primer Mix Long (96% over 25bp)
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC223920.5914605896327461Clontech Universal Primer Mix Long (96% over 25bp)
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA186350.4922234765901315Clontech Universal Primer Mix Long (96% over 25bp)
GAGTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCA185170.48910663354008393Clontech SMARTer II A Oligonucleotide (95% over 24bp)
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC178140.4705376448605635Clontech Universal Primer Mix Long (96% over 25bp)
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT176540.46631141699609235Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG166260.4391579029668648Clontech Universal Primer Mix Long (96% over 25bp)
GTATAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT161990.4278791573535572Clontech Universal Primer Mix Long (95% over 24bp)
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT157150.4150948180635318Clontech Universal Primer Mix Long (96% over 25bp)
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC156060.4122157003308608Clontech Universal Primer Mix Long (96% over 25bp)
GCTTAGGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT142640.3767682141176085Clontech SMART CDS Primer II A (96% over 26bp)
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG140490.3710892204247253Clontech Universal Primer Mix Long (96% over 25bp)
GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT126200.33334372281016683Clontech Universal Primer Mix Long (96% over 28bp)
GAGCAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT125070.330358949380884Clontech Universal Primer Mix Long (96% over 27bp)
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC123320.3257365126541186Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GAGTATAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA122110.3225404278316123Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT120920.3193971708574118Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GTACCTAAGCAGTGGTATCAACGCAGAGTACCTAAGCAGTGGTATCAACG113200.29900562141133824Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG113160.29889996571472643Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA112550.2972887163413968Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTTAGGTACTCTGCGTTGATACCACTGCTTAGGTACTCTGCGTTGATAC108300.28606279857639516Clontech SMART CDS Primer II A (96% over 26bp)
GAGTAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGA107790.28471568844459494Clontech SMARTer II A Oligonucleotide (95% over 24bp)
ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG99400.262554406080274Clontech Universal Primer Mix Long (96% over 25bp)
AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA95260.25161904148095476Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA89160.2355065477476583Clontech Universal Primer Mix Long (96% over 25bp)
GTACCTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA88680.23423867938831688Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCTTACTCTGCGTTGATACCACTGCTTGCTCTGCGTTGATACCACTGCTT86350.2280842350606807Clontech Universal Primer Mix Long (96% over 28bp)
ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA84130.2222203438987269Clontech Universal Primer Mix Long (96% over 25bp)
GCTTGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC83880.22155999579490326Clontech SMART CDS Primer II A (96% over 26bp)
CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT81010.21397919956300804Clontech Universal Primer Mix Long (96% over 25bp)
CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT74140.19583283366993476Clontech Universal Primer Mix Long (96% over 25bp)
GTACAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGA72620.19181791719868713Clontech Universal Primer Mix Long (100% over 24bp)
GAGTAAGCAGTGGTATCAACGCAGAGTACCTAAGCAGTGGTATCAACGCA72480.1914481222605459Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA71350.1884633488312631Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTTTCTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT70560.18637664882318045Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GCTTGCTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT66070.1745167968785081Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GCTTACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACTGC65920.1741205880162139Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GCTTGTACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACT64670.1708188474970958Clontech SMART CDS Primer II A (96% over 26bp)
CTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACTG63650.16812462723349542Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GAGTACAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCA62240.16440026392793014Clontech Universal Primer Mix Long (96% over 25bp)
TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA60640.16017403606345892Clontech Universal Primer Mix Long (96% over 25bp)
GAGGAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT58770.1552346322468582Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GAGTACCTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCA57020.1506121955200928Clontech Universal Primer Mix Long (95% over 24bp)
AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG54790.14472189043398606Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GAGTACAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA53460.14120883852164434Clontech Universal Primer Mix Long (96% over 25bp)
CTGCTTACTCTGCGTTGATACCACTGCTTGCTCTGCGTTGATACCACTGC51540.1361373650842789Clontech Universal Primer Mix Long (96% over 28bp)
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA50110.13236017393040775Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC49440.13059044101216044Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GTACAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT49000.12942822834943085Clontech Universal Primer Mix Long (100% over 24bp)
GCTTCCTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT47630.12580952074047738Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GAGTAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA47330.12501710301588903Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTAT47270.12485861947097135Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA46170.12195308781414739Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTTACTCTGCGTTGATACCACTGCTTAGGTACTCTGCGTTGATACCACT45860.12113425616540612Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTGCTCTGCGT45540.12028901059251186Clontech Universal Primer Mix Long (96% over 28bp)
CTGCTTACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACT43880.11590429918312299Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GAGTACCTAAGCAGTGGTATCAACGCAGAGTACCTAAGCAGTGGTATCAA43660.11532319285175818Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT43320.11442511943055805Clontech Universal Primer Mix Long (96% over 28bp)
GCGTTGATACCACTGCTTGCTCTGCGTTGATACCACTGCTTACTCTGCGT43000.11357987385766381Clontech SMART CDS Primer II A (95% over 24bp)
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA42460.11215352195340478Clontech Universal Primer Mix Long (96% over 25bp)
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT41940.11077999789745163Clontech SMART CDS Primer II A (96% over 26bp)
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGA41330.10916874852412199Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GAGTTAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT41290.10906309282751021Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GAGAAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT39750.10499534850795668Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC37980.10032008393288538Clontech SMART CDS Primer II A (96% over 26bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)