FastQCFastQC Report
Sun 11 Sep 2022
EGAF00001321601

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001321601
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2632041
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70830.2691067502367934No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49570.188332932503711No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT46200.17552918058647263Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATG35700.13563618499863794No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27630.10497556838970214No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)