FastQCFastQC Report
Sun 4 Sep 2022
EGAF00001321613

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00001321613
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1412883
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGA204811.4495892441200013Clontech Universal Primer Mix Long (95% over 24bp)
GCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACT186371.31907596028829Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GAGTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCA128300.9080723598486216Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT70570.4994751865511865Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT68130.4822055329422182Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA62760.44419813954871Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA59470.42091241808415847Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58650.4151086820352428No Hit
CTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACTG51280.36294583486389176Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA46160.32670787319261396Clontech Universal Primer Mix Long (96% over 25bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38820.27475735782793054No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT38780.2744742487523737Clontech Universal Primer Mix Long (96% over 25bp)
GTACAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGA38440.27206782161014037Clontech Universal Primer Mix Long (100% over 24bp)
GAGTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA37930.26845818089679047Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT37710.2669010809812277Clontech SMART CDS Primer II A (96% over 26bp)
GAGTACAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCA33500.23710385077886845Clontech Universal Primer Mix Long (96% over 25bp)
CTGCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCA29000.20525407977872193Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GCTTGTACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACT28660.20284765263648866Clontech SMART CDS Primer II A (96% over 26bp)
GCGTTGATACCACTGCTTATACTCTGCGTTGATACCACTGCTTATACTCT27970.19796402108313285Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTG27790.19669003024312698Clontech Universal Primer Mix Long (95% over 24bp)
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC26570.18805520343864285Clontech Universal Primer Mix Long (96% over 25bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24730.17503218596302736No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT23100.16349549113408543Clontech Universal Primer Mix Long (96% over 25bp)
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT22740.16094750945407368Clontech SMARTer II A Oligonucleotide (95% over 24bp)
TATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGG21220.1501893645829131Clontech SMARTer II A Oligonucleotide (95% over 24bp)
TGCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCAC20720.14665050113845238Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GCTTATACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC20280.14353630130732692Clontech Universal Primer Mix Long (96% over 25bp)
GTATAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT18650.13199960647838496Clontech Universal Primer Mix Long (95% over 24bp)
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC18640.13192882920949578Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG17100.12102912980055673Clontech SMARTer II A Oligonucleotide (96% over 25bp)
AGTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAG15220.10772300324938441Clontech Universal Primer Mix Long (95% over 24bp)
GGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGT15190.10751067144271678Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG14970.10595357152715405Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC14630.10354714438492077Clontech SMARTer II A Oligonucleotide (95% over 24bp)
ACGCAGAGTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCA14260.10092838543601981Clontech SMARTer II A Oligonucleotide (95% over 24bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)